5. The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data? The activity of the GAPDHenzyme was assayed in thepresence of a constantamount of glyceraldehyde-3-phosphate and anincreasing amount ofNAD. The activity of thecontrol (that is, the enzymewith no other metabolites)was compared to theactivity of the enzyme inthe presence of variousmetabolites. The results areshown in the figure to theright.Units/mg proteinR8910-NADP+Hill coeficient = 1Hill coefficient = 2NAD], mM15Control -- AMP-X-Glucose-1-P20

Enzyme are proteins molecules that catalyse the biochemical reactions. They are very crucial for…

The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data?

The researchers did not study the effects of NADH, ADP and ATP on the enzyme. Given what you know of their roles in GAPDH, classify each as an activator or an inhibitor of the enzyme. What overall consideration having to do with how glycolysis is regulated allowed you to make these inferences, without having any data?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter25: Nitrogen Acquisition And Amino Acid Metabolism

Section: Chapter Questions

Problem 16P: A Deficiency on 3-Phosphogtycerate Dehydrogenase Can Affect Amino Acid Metabolism Although serine is...

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Assume that all carbon atoms and nitrogen atoms in both alanine and aspartate are labelledwith radioisotopes. Then, the radioisotope-labelled alanine and aspartate molecules areallowed to undergo metabolism in the liver. All carbon atoms and nitrogen atoms in both ureaand glutamine are found to be labelled with radioisotopes. Please write down the metabolicreactions that lead to the incorporation of radioisotope-labelled carbon and nitrogen atoms intourea and glutamine.
The enzyme serine hydroxymethy ltransferase (SHMT) catalyses the conversion of serine into glycine. The fo llowing table gives the initial rates, vo, for the SHMT-catalysed reaction of the substrate serine at var ious concentratio ns of serine, lSI.[S]/(mmol dm-3) 10 20 30 40vo(μmol dm-3 s-1) 1.63 2.94 4.10 4.95Use the data to determine the values of the MichaelisMenten constant, the maximum velocity of the reaction, and the maximum turnover number of the enzyme.
TPCK and TLCK are irreversible inhibitors of serine proteases. One ofthese inhibits trypsin and the other chymotrypsin. Which is which? Explainyour reasoning. Suggest the effects of each of the following mutations on the physiologicalrole of chymotrypsinogen:(a) R15S(b) C1S(c) T147S
A Deficiency on 3-Phosphogtycerate Dehydrogenase Can Affect Amino Acid Metabolism Although serine is a nonessential amino acid, serine deficiency syndrome has been observed in humans. One such form of the syndrome is traceable to a deficiency in 3-phosphoglycerate dehydrogenase (see Figure 25.31). Individuals with this syndrome not only arc serine-deficient but also arc impaired in their ability to synthesize another common ammo acid, as well as a class of lipids. Describe why.
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ATP is a (+) allosteric effector, and CTP is a (-) allosteric effector of theenzyme ATCase. Both of these heterotropic effectors bind to the regulatorysubunits on ATCase. The substrates of ATCase, aspartate and carbamoylphosphate, bind the enzyme active site with positive cooperativity (i.e.,they exert a “+” homotropic effect on activity). As the concentrations ofthe substrates change from values where [S] ≪ KM to values where [S] issaturating ([S]≫ KM), how will the binding constants for each of the twoallosteric effectors change? In other words, does ATP bind ATCase withhigher affinity when [S] is low or high? Does CTP bind ATCase with higheraffinity when [S] is low or high?
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