You are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in E. coli, a prokaryote. This piece of DNA consists of a bacterial promoter, a ribosome binding sequence (RBS), a eukaryotic gene and a terminator sequence. Do you think that this piece of DNA would be expressed if placed into an E. coli cell that contains all the machinery needed for gene expression?

You are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in E. coli, a prokaryote. This piece of DNA consists of a bacterial promoter, a ribosome binding sequence (RBS), a eukaryotic gene and a terminator sequence. Do you think that this piece of DNA would be expressed if placed into an E. coli cell that contains all the machinery needed for gene expression?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 7TYU: Which of the following is characteristic of genes and gene regulation in both bacteria and...

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You are a research scientist working in genetic engineering. You create a piece of DNA that you want to express in E. coli, a prokaryote. This piece of DNA (represented by the schematic in the figure) consists of a bacterial promoter, a ribosome binding sequence (RBS), a eukaryotic gene and a terminator sequence. Do you think that this piece of DNA would be expressed if placed into an E. coli cell that contains all the machinery needed for gene expression? Fully motivate your answer.
Which of the following is the best description of a transcription factor? A. A protein that brings the correct nucleotides to RNA polymerase B. A protein that binds to specific DNA sequences C. A DNA sequence that binds to RNA polymerase D. A modification that is made to mRNA to help it be exported from the nucleus. .
f you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECT
Imagine that you and your colleagues are working in a lab to develop a protein synthesis system for a new type of synthetic cell. During your brainstorming sessions, you propose that polycistronic mRNA would be much more useful than mRNA that is only translated into one protein. This would allow for multiple proteins necessary for a particular function to be translated together. One of your colleagues says that is a good idea, but if you decide to go with polycistronic mRNA, you’d better make sure to use a prokaryotic translation system. Why might it be problematic to use a eukaryotic translation system with polycistronic mRNA? Mention three reasons:
You are a genetic engineer that is working on transcriptional regulation in living organisms. In addition to your day job, you are a video game connoisseur and are designing a cell phone game where participants design the features of an organism that is the best at up-regulating transcription of a gene, WINNING. Participants are able to pick from a variety of organisms, cis regions, trans factors and a variety of enzymes. You want to set the high score on the leader board so you play the game and design the following features into your organism. - You need to describe ALL the features you will be including into your organism as well as what organism you picked for your entry. You need to include all the components of transcription, key features of transcriptional regulation and other features that will increase transcription to the highest level. You need to explain why you included every feature and how each will contribute to increased gene expression. Be creative and error on the…
. You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You have identified the gene and place a copy of the gene on a plasmid in E. coli next to a bacterial promoter sequence. You determine that lots of mRNA is made from your gene in your E. coli system, but the protein produced is larger and doesn't have the same properties as the eukaryotic protein you expected. What mistake have you made and how can you fix it?
You are searching the sequence of a coding strand of DNA. What kinds of sequences would help you find the beginning of a protein-coding gene? Group of answer choices a promoter sequence upstream (to the 5' end) of a transcription start site and an ATG sequence corresponding to the start codon a transcription termination sequence a silencer sequence an ATA sequence corresponding to the codon for isoleucine
A biology professor at a prestigious university stated to his introductory class that "Today, a basic understanding of DNA and genetics is as important as a basic understanding of computers and the internet."Based upon what you have learned about DNA and gene expression in this module, how could you make a case to support this statement?
You are studying the rate of transcription of a particular eukaryotic gene. When the DNA located several thousand bases upstream from the gene is removed, the transcription rate of the gene decreases dramatically. How would you interpret these results?
You are studying a new gene that is expressed within the adipose tissue of humans during the development of diabetes. Specifically, you want to learn why gene expression levels are very high in those people who develop diabetes. What DNA sequence would you concentrate on? Group of answer choices A specific response elements B the TATA box C enhancer sequences D promoter proximal elements E metal response elements
if the following DNA sequence were transcribed, which of the following describes the output of this process? 3'- TCTGGACA-5' A. This would produce a protein that looks like 5'- A G A C C U G U -3' B. This would produce a tRNA that looks like 3'- A G AC C U G U -5' C. This would produce an mRNA that looks like this: 5'- A G AC C U G U -3' D. This would produce an mRNA that looks like 3'- U C U G G A CA -5' E. This would produce another strand of DNA that 0ok like 5-AG ACCT GT-3. ..
You are interested in a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli in order to produce large amounts of the protein. You take your DNA construct and place it into E. coli. You measure protein expression and notice that no protein is being made. Provide 1 reason why you don't see protein as it relates to transcription and how this problem could be resolved.