Your research project involves adding a salamander gene to a bacterial cell so that it gets translated into a protein for biochemical analyses. Point out three things that you will need to change in the sequence of this typical eukaryotic gene in order for it to be correctly transcribed and translated in a bacterium. Place gene inside expression vector, then insert CDNA into expression vector, and finally encode with ribosome binding site near initiation codon.

Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…

A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…

Q: What enzyme is used to make cDNA from pre-mRNA?

A: c DNA or complementary DNA is a type of double stranded DNA which is made from single stranded RNA.…

Q: Shown below is the genomic structure of the human B-globin gene. The numbers within the boxes…

A: Transcription is the process by which DNA act as template for the formation of mRNA . This process…

Q: From the list given - choose all the regulatory sequences that you thỉnk would control the…

A: Introduction Gene expression in eukaryotic cells is regulated by repressors as well as by…

Q: A full-length eukaryotic gene is inserted into a bacterial chromosome.  The gene contains a complete…

A: The gene expression of eukaryotes is regulated during transcription and RNA processing, which take…

Q: A bioengineer takes the gene from the human liver cell and insert into a bacterial chromosome. Then,…

A: Introduction  A genome is consists of transcriptionally active genes. These genes form mRNA as they…

Q: A certain gene in a bacterium codes for a polypeptide that is 400 amino acids long. How many…

A: Introduction  Short strands of amino acids connected by peptide bonds are called peptidides.…

Q: A bioengineer takes the gene from the human liver cell and insert into a bacterial chromosome. Then,…

A: It is related to molecular cloning in which a gene of interest cloned into the desired vector.

Q: Figure 2 is a schematic drawing of ABC gene, which encodes ABC protein. Transeriptional terminator…

A: Introduction: Gene is a unit of heredity. It contains the genetic information. It is made up of DNA.…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: Because of the remarkable efficiency with which DNA molecules are introduced into cells, E. coli is…

Q: If you want to express a eukaryotic gene in a bacterial cell, you ned to (select all that apply):…

A: The gene is a basic physical and functional unit of heredity. Genes are made up of DNA.The gene is a…

Q: You are working in a research lab that is surveying the CFTR gene sequence in humans to identify new…

A: A synonymous mutation is mutation in the DNA sequence that leads to change in the codon for amino…

Q: You are teaching a class on the regulation of eukaryotic gene expression. such as the promoter…

A: Binding of RNA polymerase II  and transcription factors is necessary for transcription.

Q: Scientists developed a marker technology to examine the function of RNA polymerase in yeast cells.…

A: Transcription process is responsible for producing mRNA. It takes place in the nucleus.

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: The incorporation of a foreign gene into E. Coli genome and its expression is the process of genetic…

Q: You perform a translation experiment with mRNA and all required molecules in two identical test…

A: It is the process in which a RNA is coded to form a protein.  The mRNA formed from the corresponding…

Q: NASA has identified a new microbe present on Mars and requests that you determine the genetic code…

A: Introduction RNA is an abbreviation for ribonucleic acid, a nucleic acid found in all living cells.…

Q: In transcription, the location where RNA polymerase initially attaches to a gene is called the (a)…

A: Transcription is that the first step of organic phenomenon. Throughout this method, the…

Q: Consider a human lover cell vs a cheek cell: Explain whether the following components are…

A: Introduction: Transfer ribonucleic acid (tRNA) is a type of RNA molecule that helps decode a…

Q: You perform a translation experiment with MRNA and all required molecules in two identical test…

A: Puromycin is a tyrosyl-tRNA mimic that works by identifying and releasing elongating polypeptide…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: Expression of eukaryotic gene in prokaryotic organisms like bacteria convey several challenges. The…

Q: You have cloned a novel E. coli gene and wish to express protein from it in human cells. In order to…

A: The Central Dogma theory explains that DNA forms DNA by Replication, DNA forms RNA by Transcription…

Q: w is the diagram of a eukaryotic gene that encodes a protein. The promoter and n start sites are…

A: Post transcriptional modification removes introns and add existing exons together in the primary RNA…

Q: Sometimes errors occur during transcription or translation. each amino acid is coded for by several…

A: This suggests that the genetic code is degenerate which means that each codon is specific for only…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: Gene expression  It is defined as the process through which the nucleotide sequence of the gene is…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: Gene expression is a phenotypic expression of genes through writing and translation processes.…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: The process of transferring genetic information from the DNA into the RNA molecule is called…

Q: What is a promotor? Is it located upstream or downstream from a gene?

A: According to bartleby guidelines when multiple questions are posted we are allowed to answer the…

Q: You are examining a set of genes from a phage that are transcribed late in infection. You know that…

A: Transcription: It is a process of synthesis of mRNA transcript from the double-stranded DNA as a…

Q: CRISPR in nature evolved as a "bacterial immune system" or "genetic vaccine" to target infecting…

A: CRISPR stands for "Clusters of Regularly Interspaced Short Palindromic Repeats". Cas9 is the…

Q: man rhodopsin gene is 2675 nucleotides long from transcription start site to transcription stop…

A: The rho factor provides directions for creating a macromolecule referred to as rhodopsin. This…

Q: You are studying a bacterial plasmid that contains 3 operons and 9 genes. How many transcriptional…

A: In bacteria, similar and related genes are often found in a cluster on the chromosome, where they…

Q: Think of the eukaryotic gene expression process. Which column below accurately describes which…

A: RNA polymerase is a molecular enzyme that synthesizez RNA molecule from a DNA template. RNA…

Q: A scientist mutates eIF-2 to eliminate its GTP hydrolysis capability. How would this mutated form of…

A: Selection of the AUG start codon for translation in eukaryotes is governed by codon-anticodon…

Q: Microbiologists describe the processes of transcription and translation as “coupled” in bacteria.…

A: DNA serves as a genetic material and carries genetic information from one generation to another. DNA…

Q: This sequence given is of one strand of eukaryotic DNA containing a hypothetical gene. The base that…

A: Answer :  DNA---> mRNA ----------> PROTIENS (AMINO ACIDS)  A=T, G=C(DNA), A = U(mRNA) From…

Q: Which of the following characteristics is typical of a eukaryotic gene that can be transcribed? a.…

A: A cell is a basic membrane-bound unit and it is often called the basic building blocks of all living…

Q: You see partial sequence of a gene as follows (non template strand shown)…

A: Transcription is a heterocatalytic action of DNA by means of which RNA is synthesized from specific…

Q: Below is the double stranded DNA sequence of part of a hypothetical yeast genome encoding a very…

A: Introduction Transcription : It Is The Initial Stage In Gene Expression, Which Involves "The…

Q: Some viruses that infect E. coli produce an “anti-terminator” protein that causes RNA polymerase to…

A: RNA polymerase is the enzyme that is involved in the synthesis of RNA from the DNA template strand…

Q: Here is a schematic map with a scale of a eukaryotic gene. How long is the primary mRNA transcript…

A: The mRNA is produced by the process of transcription.

Q: The antibiotic kasugamycin blocks the binding of methionine to tRNA. From this information, you can…

A: In both prokaryotes and eukaryotes the first step of the initiation stage is the binding of a…

Q: Figure 2 is a schematic drawing of ABC gene, which encodes ABC protein. Transeriptional terminator…

A: Transcription The transcription process involves the creation of mRNA from DNA using the enzyme RNA…

Q: Many regions of non-coding eukaryotic DNA previously thought to be "junk" are now known to contain…

A: Genes are the functional unit of heredity. They carry information that gets expressed and results in…

Q: You are a research scientist working in genetic engineering. You create a piece of DNA that you want…

A: Given: This piece of genetically engineered DNA (represented by the schematic in the figure)…

Q: Besides the examples listed in Table 24.3, list five types ofshort sequences that a geneticist might…

A: Short sequences refer to the DNA (deoxyribonucleic acid) sequences that are usually up to few bases…

Q: Which feature of promoters can be found in both prokaryotes and eukaryotes? a. GC box b. TATA box c.…

A: Transcription is a process by which the information a strand of DNA is copied into a new molecule of…

Environmental health and hazards

An organism's environment is the environment in which it lives and has an impact on its well-being. The World Health Organization (WHO) defines environmental health as "those aspects of public health, such as quality of life, that are influenced by physical, chemical, biological, social, and psychosocial components in the environment." Environmental health is concerned with both natural and man-made settings in order to enhance public health. Environmental science, occupational medicine, toxicology, and epidemiology are the key subdisciplines of environmental health.