You are trying to isolate a mutant of wild-type E. coli thatrequires histidine for growth. This can best be done using1. direct selection.2. replica plating.3. penicillin enrichment.4. a procedure for isolating conditional mutants.5. reversion.a) 1, 2    b) 2, 3    c) 3, 4    d) 4, 5    e) 1, 5

Q: When a company changes from the LAL assay to the Recombinant Factor C assay, they must perform a…

A: LAL or Limulus amebocyte lysate assay is a technique used to assess and quantify the presence of…

Q: Which of the following objects would you use microinjection in order to transfer DNA into? a. Egg…

A: Microinjection is the process of transferring DNA materials into a living cell. It can be done by…

Q: When a virus mistakenly picks up a segment of host bacterial DNA, packages it into a viral particle,…

A: There are many methods of DNA transfer in bacteria such as transduction, conjugation and…

Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…

A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…

Q: All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1…

A: The Luria-Delbruck Experiment ---This experiment performed in 1943  demonstrate that in bacteria…

Q: Discuss how recombinant DNA technology has improved the production of the following: Restriction…

A: There are few important points about recombinant DNA technology . Which are as follows . It is also…

Q: The cath sample was both filtered to remove all bacteria, and then treated with DNase.  Based on…

A: A cath or catheter is a thin hollow tube, which is a medical equipment that is used for various…

Q: Why would it have been possible to utilize N14 in the original bacteria and only allow growing…

A: To build  new F1 bacteria one strand from parent strain and one from the new N15 strain  is required…

Q: Why must genetically altered bacteria be grown in a carefully controlled environment?

A: Introduction: The living organisms whose genetic material has been falsely controlled in a lab…

Q: Why are bacteria excellent hosts for recombinant DNA experiments?

A: Recombinant DNA technology is the branch of biotechnology associated with the joining of DNA…

Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

A: The technology used for joining different DNA molecules from different species is known as…

Q: If you plated 0.1mL of a 1:100 dilution of phage stock onto a plate with an E.coli lawn, what is…

A: In microbiology lab dilution factor represent the solute vs solvent ratio for making the the culture…

Q: You perform an Ames test to determine which compound X causes mutations or not. You grow an…

A: Technique is developed by bruce ames. Ames test is generally used to detect the mutation in the test…

Q: A patient has a Haemophilus influenzae respiratory infection. She is prescribed Amoxicillin.…

A: The various modes of horizontal gene transfer in bacteria are transduction, transformation, and…

Q: You have two strains of E. coli, Strain I and Strain II. These strains were also co- cultured on a…

A: Antibiotics are those substances that kill or prevent the growth of bacteria. Antibiotics can be of…

Q: In the phage titer experiment, why did you plate multiple dilutions?   a. so that you should have a…

A: Bacteriophage  It is a type of virus that infect bacteria.

Q: The figure illustrates the results of RT-qPCR. Which of the following statements about the graph is…

A: Quantitative ECR is the type of PCR in which the amount of PCR product can be determined in real…

Q: In the experiment of Avery, McLeod, and McCarty, the additionof RNase or protease to a DNA extracta.…

A: Avery, McLeod, and McCarty were the three scientists who performed an experiment and reported their…

Q: If HIV was just an enveloped virus, but not a retrovirus, which of the following drugs would likely…

A: Virus captures the host replication machinery and multiplies its genome. This is the foundation of…

Q: Outline how you would use the technique of replica plating toshow that antibiotic resistance is due…

A: Microorganisms possess many types of resistance they are antimicrobial, antibiotic, and antifungal.…

Q: A DNA fingerprint is based on _____. a repeating sequences found on noncoding regions b the…

A: DNA fingerprinting is commonly employed in investigations to connect evidence with suspects, but DNA…

Q: explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the…

A: Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host…

Q: In DNA cloning, why do we need to check colonies for insert after transformation?

A: Gene is the basic unit of heredity. All living organisms contain certain nucleic acids as their…

Q: Number of cycles 1 сycle Step Temperature Time Initial Denaturation 98°C 5 minutes Denaturation (a)…

A: Polymerase Chain Reaction requires 5 most important aspects before starting with the protocol, these…

Q: You mix two auxotrophic strains of bacteria; one is leu+ trp+ his– met– and the other is leu– trp–…

A: Transduction is a process where the bacterial genes are transferred via the use of bacteriophage…

Q: If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type…

A: The incorporation of recombinant DNA into the bacterial cell is known as transformation. It leads to…

Q: Discuss TWO (2) methods of transformation of competent bacterial cells with the plasmid in Figure 1.

A: The genetic modification of a cell caused by the direct uptake and assimilation of external genetic…

Q: Based on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which…

A: Antibiotics are substances that have the ability to kill bacteria or restrict their growth. They are…

Q: You have two E. coli strains, XL10 and K12. XL10 is ampicillin resistant, or AmpR, and can grow on…

A: The plasmid is the extrachromosomal material found in bacterial cells. They contain various…

Q: The following image is of an agarose gel. If DNA samples were loaded to this gel and the…

A: Electrophoresis is a method that uses an electric field to separate macromolecules in a fluid or gel…

Q: You performed an experiment where you want to introduce a new gene into bacteria using the pGLO…

A: The pGLO plasmid is an engineered plasmid that is used as a vector in biotechnology to create…

Q: Which of the following types of data produce curves similar in shape to the graph? Select all that…

A: ANSWER 6. Osmotic fragility test   It is the test that measures the resistance to hemolysis of RBC…

Q: what are the plasmid status of bacterial cells resulting from conjugation between a f+ and a f-…

A: Gene transfer is the method of inserting genetic material (DNA or a gene of interest) into cells…

Q: you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase…

A: Escherichia coli is a Gram Negative and facultative anaerobic bacteria. Competent cells are the…

Q: Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as…

A: Intrinsic resistance can be described as "it is a natural resistance property, which is derived…

Q: We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender…

A: Conjugation, transduction and transformation are the three processes that are responsible for…

Q: Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay…

A: This assay is used to measure the concentration of protein.

Q: In the bacterial transformation experiment, what is the primary purpose of using an…

A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…

Q: During the Ames test, his− bacteria are exposed to a mixture of chemical compounds extracted from…

A: The Ames test, named for its developer, Bruce Ames, is a method to test chemicals for their…

Q: Recent scientific studies show that the plasmid plays a vital role in gene transfer not only in the…

A: A plasmid is circular, extrachromosomal, small piece of DNA present in the cytoplasm that is…

Q: Is the DNA you extracted is pure? What are the possible impurities?     What can we do with the…

A: DNA represents deoxyribonucleic corrosive, which is an atom that contains the guidelines an organic…

Q: Both viruses and short bacterial fragments from lysed bacteria can pass through the filter in the…

A: Genetic recombination is a process when the genetic content of either the same or different…

Q: In a molecular biology laboratory, a student obtained competent E. coli cells and used a common…

A: Kanamycin is an aminoglycoside antibiotic.

Q: Part A: Antibiotic resistance is a major health concern. Resistance to various antibiotics can be…

A: When the existence of antibiotics and antifungals forces bacteria and fungi to adjust, antimicrobial…

Q: Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml…

A: Bacteriophages are viruses that infect bacteria. When the bacteriophages are added to the bacterial…

Q: a bacteriophage has been isolated from the growth chambers that has evolved the capability to the…

A: In the modern age of technological revolution, various different techniques of genetic engineering…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.