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Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml O 2.37 x 10^11 pfu/ml O 2.37 x 10^5 pfu/ml 23.7 x 10^8 pfu/ml

Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml O 2.37 x 10^11 pfu/ml O 2.37 x 10^5 pfu/ml 23.7 x 10^8 pfu/ml

Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter3: Basic Hemostasis
Section3.6: D-dimers
Problem 8RQ
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Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? O 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml 2.37 x 10^11 pfu/ml 2.37 x 10^5 pfu/ml O 23.7 x 10^8 pfu/ml
Transcribed Image Text:Making a series of dilutions, you transfer 40 ul (microliters) from a Bacteriophage-T4 stock to 4 ml (4000 ul) of Tryptone Soy Broth (TSB) in tube A. From tube A, you mix and transfer 40 ul to 4 ml TSB in tube B. You repeat this process from Tube B to Tube C, and then from Tube C to tube D. You mix 100 ul (0.1 ml) of the Tube D phage dilution with 100 ul of a dense culture of susceptible E. coli in molten soft agar, and plate to grow overnight at 37*C. The next morning, you count 237 plaques from your plating of the tube D phage dilution. What was the original concentration of phage particles in your Bacteriophage-T4 stock culture? O 237 x 10^10 pfu/ml 2.37 x 10^8 pfu/ml 2.37 x 10^11 pfu/ml 2.37 x 10^5 pfu/ml O 23.7 x 10^8 pfu/ml
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