You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to cut all 15 with PstI. The easiest way to set up the digestions is to make a mix and add some to each tube. Again, give ingredients and how much of each, and how much of the mix you would add to each tube. You will be using a 20 ul volume/tube. (Hint: DNA is NOT part of the mix, because each tube has a different DNA to cut, so you have to put that in separately. But the mix includes water, buffer, and enzyme.)
You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to cut all 15 with PstI. The easiest way to set up the digestions is to make a mix and add some to each tube. Again, give ingredients and how much of each, and how much of the mix you would add to each tube. You will be using a 20 ul volume/tube. (Hint: DNA is NOT part of the mix, because each tube has a different DNA to cut, so you have to put that in separately. But the mix includes water, buffer, and enzyme.)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to cut all 15 with PstI. The easiest way to set up the digestions is to make a mix and add some to each tube. Again, give ingredients and how much of each, and how much of the mix you would add to each tube. You will be using a 20 ul volume/tube. (Hint: DNA is NOT part of the mix, because each tube has a different DNA to cut, so you have to put that in separately. But the mix includes water, buffer, and enzyme.)
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