Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him.   You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel. L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene

Q: You are using a spectrophotometer to determine the concentration of DNA in a PCR product, but your…

A: The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A260)…

Q: COMPONENT 1 REACTION N+1 REACTIONS 15.80 µL 2.50 µL 1.50 µL 2.00 µL 1.00 µL 1.00 µL 0.15 µL…

A: Polymerase chain reaction is a procedure that is used to amplify a particular segment of DNA. This…

Q: Which of the following enzymes are needed to introduce foreignDNA into a vector?a. DNA gyrase and…

A: The tightly packed genetic material located in the nucleus of a living organism which is made up of…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: The human genome is made from more than 3 billion nucleotides.  How many sites (loci) are…

A: According to the question,  The human genome is made from more than 3 billion nucleotides.  How many…

Q: The primers used in PCR are double stranded DNA oligosaccharides. True False

A: The answer is False.  Primers are small, chemically synthesised oligonucleotides that are…

Q: If you placed one copy of your gene into a tube and ran a PCR for 3 cycles, how many copies of your…

A: Answer: PCR : PCR is the Polymerase Chain Reaction that is used to multiply the sequence of DNA in…

Q: When you collect your cheek cells and prepare the DNA, is it really a problem that bacteria and food…

A: Answer: Cheek cells are the cells in buccal cavity whose DNA can be studied and it is easily…

Q: • Was the negative electrode or the positive electrode positioned closer to the bottom of the gel? •…

A: A microsatellite is a sequence of DNA, it does not code for protein means they are present in the…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: 2. To tube 1, add 10 μl of 50:50 AT:GC DNA (tube B). 3. Add 5 μl of Buffer to tubes 2-7. 4. Remove 5…

A: The results of a polymerase chain reaction (PCR) are significantly influenced by the overall amount…

Q: When you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold…

A: Gene expression: The process in which genetic information encoded in genes is expressed in the form…

Q: You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to…

A: A reaction mixture is a combination of 2 or more reactants in definite proportions to carry out a…

Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? A DNA…

A: DNA quantity was determined by several methods where absorbance was one of the widely used methods…

Q: DNA fingerprinting and Restriction Fragment Length Polymorphism (RFLP) analysis are often used to…

A: Gel electrophoresis technique is used to seprare the DNA fragments according to their size and mass…

Q: why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only…

A: RFLP [restriction fragment length polymorphism]-- will create several fragments of DNA because DNA…

Q: Linkage analysis ________.   A. is used to create a physical map B. Involves breaking and…

A: Genomics involves the study of the entire genome which includes the complete set of genes,…

Q: R. Alu 100 100 300 F ----- 100 100 Where would you expect to see a band or bands if you used PCR to…

A: 500 bp template was prepared by using amplified PCR with forward and reverse primers of 100 bp each…

Q: w many DNA molecules would exist after 30 PCR cycles?

A: The PCR reaction is one of the breakthroughs in the field of biotechnology. The discovery of PCR has…

Q: 22 22 22 16 22 7. Black Blue Purple Orange Green Gray | | | | || || || | || || || | || 8. | | || 7.…

A: Simple sequence repeats (SSRs) are also called microsatellite VNTR (variable number tandem repeat).…

Q: A researcher used polymerase chain reaction (PCR) to test four individual nematodes for the presence…

A: A diploid organism is said to be homozygous for a gene when it contains two copies of the same…

Q: DNA fingerprinting involves using restriction enzymes to cut DNA at a specific sequence, resulting…

A: DNA fingerprinting: DNA fingerprinting is a technique that involves the isolation of genetic…

Q: IF YOU WERE TO CREATE RECOMBINANT DNA OF YOUR OWN, WHAT GENE COMBINATION WOULD YOU LIKE TO HAVE AND…

A: Recombinant DNA molecules are DNA molecules with a new set of genes inserted into their nucleotide…

Q: Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

A: The term SSR stands for simple sequence repeat that is a microsatellite VNTR (variable number tandem…

Q: Please answer these two questions regarding PCR   a) First question you must use the image: What is…

A: The DNA profile of suspect B is TH01 6-10. One allele has 6 repeats and other allele has 10 repeats.…

Q: In this gel, PCR is performed using primers outside of the repeat area anf the product is then run…

A: Gene is the sequence of nucleotide that encodes a protein.

Q: write a short note on different available methods for DNA SEQUENCING? proper explanation and diagram

A: The process of determining the order of nucleotides (basically, the order of four nitrogenous bases-…

Q: If you had the ability to do gene editing with ONE gene for the betterment of human kind, which one…

A: Hemophilia is a hereditary problem that causes interior and outer draining by keeping blood from…

Q: What is a? 3' 5' a b. 5' d 3' e f a 5' 3' .3' 5' Select an answer and submit. For keyboard…

A: Answer : Option (a) template strand is right answer. a is template strand.

Q: What is the formula used to calculate the number of DNA molecules which will be created for a given…

A: PCR or polymerase chain reaction is a technique of DNA amplification. Here a small amount of DNA is…

Q: In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of…

A: DNA (Deoxyribonucleic acid) is a double helical structure that consists of nucleotide. Nucleotide…

Q: What is the probability that any random male in USA would share the same genotype as the murderer…

A: SSR (simple sequence repeats) or microsatellite refers to a tract of repetitive co-dominant…

Q: What is meant by the term DNA fingerprinting?

A: Every cell of a human possesses its genetic material i.e. DNA enclosed inside a membrane-bound…

Q: You have a 1.270 ug/uL DNA stock. What volume will give you 10 ug?

A: In this question, we are given a concentration of DNA that is 1.270 ug/ul. We have to determine the…

Q: Which two methods are most often used in DNA fingerprinting?

A: DNA molecules contain polymorphism. They contain variable number of tandem repeats (VNTRs) in…

Q: The diagram below represents results of agarose gel electrophoresis performed after PCR…

A: Please follow step 2 for detailed explanation.

Q: You are studying a genetic disease and trying to determine its location on a chromosome using…

A: Gel electrophoresis is used to separate the DNA fragments on the basis of their size.

Q: At the end of an experiment, you extract DNA from ten yeast colonies. You divide the DNA from each…

A: Replication replicates are the tubes having same set of conditions for reducing the variability in…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Simple sequence repeats (SSRs) are found in most organisms and occupy about 3% of the human genome.…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: PCR, including Real-Time PCR which is probably the most widely used molecular technique especially…

Q: DNA fingerprinting involves using restriction enzymes to cut DNA at a specific sequence, resulting…

A: DNA fingerprinting is a technique to distinguish between individuals of the same species using…

Q: Arrange the following steps in the PCR experiment 1 3 5. Add samples and reagents Extend Anneal…

A: (According to our guidelines we are required to answer the first question in case of multiple…

Q: What does it mean if the closest matched organism that is returned from a BLAST search is less than…

A: Basic Local Alignment Search Tool (BLAST) is a bioinformatics tool. This is a method of comparing…

Q: What molecular biology technique is used to amplify DNA from an evidence sample?     Reverse dot…

A: Gene Amplification:  Gene amplification is a term that describes a variety of natural and artificial…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Hello! Since you have posted multiple questions, we are answering only first question. If you need…

Q: Wells Base pairs: 6650 4973 4.3cm 3148 2396 1585 8 Ocm 692 > 10 Acm DNA size Markers Vector-EcoRI…

A: Hi, Thanks For Your Question.  Answer : First, Let Us See The Ladder Size. Let's Do Rounding Off…

Q: You isolate bacterial DNA from an unknown species, and use a PCR primer that amplifies DNA only from…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.