In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are sorted is Select one: O 1. log2(n) O 2. nlogn O 3. n O 4. None of above
Q: Which of the following ingredients does not belong in a sequencing reaction? (A ddNTPs B Primer C…
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A: Introduction: A primer is a short strand of DNA or RNA that is usually about 18-22 bases and serves…
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A: Chargaff's rule has been stated for the DNA, which states that the ratio of purine and pyrimidine…
Q: Refer to the attached DNA model. In the spaces provided on the attached table, identify the…
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Q: Which of these is not a tool for comparing DNA sequences? PLINK Fasta BLAST A dotplot e.g. dotlet
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Q: In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are…
A: Indexing of genome is done in order to index all the small sequenes within the large genome.The…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…
Q: Why are the first 20 bases typically ignored from a Sanger sequencing .ab1 file? The data quality is…
A: The data quality is too low and the data is noisy
Q: L 1 2 3 Locus A Locus B L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene %3D…
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Q: you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level…
A: Effect on gel profile when we either forgot to add ddNTPs Or add more ddNTPs in test tubes during…
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A: Genes are located on chromosomes. These genes can not be targeted without using any specific gene…
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A: DNA extractions is a method to isolate DNA from the nucleus of given cell culture. The four steps of…
Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…
A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…
Q: In the following drawing, the top strand is the template DNA, andthe bottom strand shows the lagging…
A: The fragments of DNA synthesized by DNA polymerases during lagging strand synthesis are described as…
Q: Which enzyme is used in Sanger sequencing reactions? O A. DNA polymerase O B. S1 endonuclease O C.…
A: Introduction :- Sanger DNA sequencing is commonly employed in research to target smaller genomic…
Q: Why are the first 20 bases typically ignored from a Sanger sequencing ab1 file? O The data quality…
A: The most commonly used tool for detecting SNVs is Sanger sequencing, it involves DNA polymerase…
Q: When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel…
A: Gel electrophoresis technique of separation of molecules on the basis of their size and charge under…
Q: When running a gel during a Sanger sequencing, the following results were obtained: I I Find the DNA…
A: Sanger sequencing is a technique which is used to determine the order of the four nucleotide bases…
Q: The following DNA fragment was sequenced by the Sanger method. The red asterisk indicates a…
A: DNA polymerase is an enzyme that catalyzes the synthesis of DNA molecules from nucleoside…
Q: A DNA sample was sent off for Sanger sequencing. The results are shown below. What is the sequence…
A: The Sanger sequencing is also known as the chain termination method, it is a method used to…
Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…
A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…
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A: Single-strand DNA-binding protein (SSB) is a protein found in Escherichia coli (E. coli) bacteria.…
Q: In this diagram of a DNA electrophoresis gel, which sample contains the smallest molecules? АВСD в с…
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Q: Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either…
A: Electrophoresis is defined as the motion of dispersed particles relative to a fluid under the…
Q: The Watson-Crick double helix immediately suggested that DNA is replicated in a manner. a. semi…
A: The genetic material is transmitted to the next generation through DNA. A cell leads to several more…
Q: 100 F В A 75 80 85 90 95 100 105 110 115 120 125 Temperature (°C) A) What is the tm of the most…
A: DNA is a double-stranded helical molecule. The monomer of DNA is a nucleotide. A nucleotide…
Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…
A: DNA: The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…
Q: These sequences are obtained as part of a human genome sequencing project using a library of 150 kb…
A: Contigs are the overlapping sequences present on DNA fragments. Each set of contigs is counted as…
Q: Which of these is not a tool for comparing DNA sequences? O PLINK O A dotplot e.g. dotlet O Fasta O…
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Q: An individual’s unique set of_______ can be used in DNA profiling. a. DNA sequences c. SNPs b. short…
A: Introduction In the genome, there is usually two types of nucleic acid sequence present. One is…
Q: What is a DNA Ladder?
A: Answer :: 1) DNA or deoxyribonucleic acid is the molecule that contains genetic code of organisms.…
Q: You are studying a genome that has 42% G:C content and the remaining 58% of the genome is As and Ts.…
A: Restriction enzymes The enzyme that is use to cut the DNA at specific site.
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: Given wheat 200 milligram germ extraction volume. = 500 microlitre Sample used for analysis=…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA generally stands for Deoxyribonucleic acid It is generally double standard Purine and…
Q: You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A…
A: Nucleic acids strongly absorb UV light with wavelengths of 260 nm due to the resonance structure of…
Q: Which of the following DNA has a high G-C content? (Tm refers to the melting temperature of the DNA.…
A: In double stranded DNA the adenine binds with thymine and guanine binds with cytosine. This four…
Q: (AKS 8a2, DOK 1) A gel from gel electrophoresis is shown below B. Which DNA Fragment is the…
A: Gel electrophoresis is an analytical technique which is used for the separation of DNA; RNA and…
Q: Humans have very similar DNA sequences, with approximately 999/1000 letters being One possible way…
A: The human genome is a complete collection of nucleic acid sequences for humans, encoded as DNA in…
Q: The 260/280 ratio of pure DNA is between 1.8- 35 2.0. The absorbance of DNA at different 30…
A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…
Q: Which of the following is the correct order in extracting the DNA? I. Washing of DNA II. Tissue…
A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…
Q: Use the set of gene sequencing results below to answer the question that follows: A G C T -Wells I…
A: Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins…
Q: Figure 3: BestrictriOR site map showing the following: A) linear DNA that is not cut as reference B)…
A: Restriction enzymes cut the DNA (deoxyribonucleic acid) at specific sites. These enzymes recognize…
Q: The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is dependent…
A: Agarose gel electrophoresis: It is used for the separation of DNA molecules that are larger than…
Q: To achieve a 10x depth coverage for a genome of length 10 Mbp, how many reads of 100 bp are…
A: A Coverage is multiplier based on total size of the genome. Formula = Coverage = (read length) x…
Q: Below is a nucleotide base pair from B-DNA with three different regions indicated by A, B, and C. C…
A: If we represent the nitrogen base as a triangle, there are going to be three edges: Hoogstein Edge:…
Q: The diagram illustrating the polymerase chain reaction (PCR) technique is provided below. How does…
A: Polymerase chain reaction (PCR) is a technique used to produce a large number of copies of a…
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- During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicaseDNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?Please explain it simply, and don't over-explain. Thanks! A. How can DNA be used to pass on inheritable material & make proteins?
- Can somone check my answers are in bold please for both questions :Which is NOT true of the different conformations of DNA? A. Z-DNA is a left-handed spiral. B. Both A-DNA and B-DNA are dehydrated. C. A-DNA and B-DNA are right-handed spirals. D. A-DNA and Z-DNA segments are limited to short regions of DNA. For double-stranded DNA, consider the following base ratios: A/G C/T C/G (A+C)/(G+T) (A+G)/(C+T) (A+T)/(G+C) Which of those ratios always equals 1? A. 3, 4, and 5 B. 3 and 6 C. 1, 4, and 5 D. 4 and 6 E. 1 and 2Please ASAP. Thank you. What is the mechanism of action for CRE recombinase in the CRE-loxP system?To determine the reproducibility of mutation fre-quency measurements, you do the following experiment.You inoculate each of 10 cultures with a single E. coli bac-terium, allow the cultures to grow until each contains 106cells, and then measure the number of cells in each culturethat carry a mutation in your gene of interest. You were sosurprised by the initial results that you repeated the experi-ment to confirm them. Both sets of results display the sameextreme variability, as shown in Table Q5–1. Assuming thatthe rate of mutation is constant, why do you suppose thereis so much variation in the frequencies of mutant cells indifferent cultures?
- LO 64- Explain how Next gen sequencing is applied in different technologies In which of the following scenario would it be best to use Next-gen sequencing? (select all that apply) a-To separate fragments of DNA based on their size- b-To insert a mutation at random in a gene c-To idently a novel pathogen d-To determine the nucleotide sequence of a microbe e- To insert methyl groups to cytosines in a DNA sequence asap pleaseMay you please help me with this? A sample of purified DNA was incubated with deoxyribonuclease (DNAse) at 37oC. An aliquot was removed from the reaction mixture every minute for 5 minutes and the A260 recorded. The following data were obtained. Time (min) A260 0 0.60 1 0.64 2 0.67 3 0.70 4 0.72 5 0.73 Describe the action of deoxyribonuclease on DNA and explain the increase in A260.Q21: Polymerase Chain Reaction (PCR) (Links to an external site.) is an essential technique for the study of DNA. PCR uses a DNA polymerase enzyme from Thermophilus aquaticus (Links to an external site.) to amplify DNA extracted from any sample. In order for the reaction to work, researchers must transfer microliters of sample to the reaction vessel for PCR. If a researcher has a DNA sample with a concentration of 0.2 micrograms per microliter, how many microliters of DNA must be transfered to conduct a 50 microliter reaction if the final concentration should be 2.3 nanograms per microliter? Report your answer to two decimal places using standard decimal notation (e.g. 0.5, not 5.0e-1)
- True or False? 454 platform sequencing involves attaching single -stranded DNA fragments to beads, which are embedded within a 96-well plate . The plate is exposed to separate washes of single nucleotides , which are used by DNA polymerase to synthesize complementary DNA. Each time a nucleotide is added, a pyrophosphate is released.Please ASAP. Thank you. Regarding the double helix of DNA, which of the following is true? a. Guanine pairs up with cytosine with three hydrogen bonds b. Complementary strands of DNA are held together by covalent bonds c. The backbone consists of ribose sugars H-bonded to phosphate groups d. Uracil pairs up with adenine with two hydrogen bondsDescribe the main technique for amplifying a segment of DNA (like the one you suspect is involved in Lee’s cancer) from a complex mixture of genomic DNA. Remember that the entire human genome sequence is known. (Hint: This is a technique that is commonly used by laboratories that do genetic testing and various other applications of molecular biology.)
