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You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volume
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- To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionYou dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?
- If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40 bacteria what would be the dilution scheme of using 4 dilutionsYou place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.
- You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What should be your initial volume from the original culture? Show solution.Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?
- If you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show workDilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture would you add to the new media?
