Answered: Dilute cells from an old culture 1:200…

Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture would you add to the new media?

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter42: Diagnostic Medical Sonography

Section: Chapter Questions

Problem 7RQ

See similar textbooks

Related questions

Q: hello, can you draw the following on endospore staining in 1000x objective? thank you. - 1 day…

A: Bacillus is a genus which is a endospore forming, rod shaped, gram positive bacteria.

Q: If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it…

A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: nd cell line same

A: Cell culture is that the culture of cells is directly isolated from parental tissue of interest;…

Q: Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive…

A: Introduction: Gram staining is a method developed by Danish bacteriologist Hans Christian Gram in…

Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…

A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…

Q: There are five bacteria in a petri dish and its number triples every minute. How many bacteria are…

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: diluted a bacterial culture a million-fold. you then plated out 0.1 mL of dilution and after…

A: The growth in a body-temperature incubator of micro-organisms on a medium of culture, like agar, for…

Q: You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted 35…

A: Given Volume plated = 0.1ml Total dilution = 108 Number of colony = 35

Q: The laboratory that you are working in does not have a microscope or motility medium. Now, how will…

A: The motility of a bacterium is determined by the presence and absence of flagella. The flagellum is…

Q: If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of…

A: In the given example, 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies. The number of cells…

Q: What if the purpose of bacterial culture? Why might it be useful to grow bacteria?

A: According to question what is the purpose of bacterial culture. Why might it be useful to grow…

Q: Bacillus cereus divides every 30 minutes. You inoculate a culture with exactly 100 bacterial…

A: When bacteria grow by the binary fission, the bacterial population increases by the geometric…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells…

A: We shall be performing a dilution calculation for this question. We know, C1V1 = C2V2 C1 is the…

Q: If a bacterial culture is started with 50 cells, what will be the cell number after four…

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…

A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…

Q: Why after a certain period the growth rate of the culture decreases until growth stops?

A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…

Q: PART B Re-watch the video and explain why the following steps are necessary: • Why use tweezers to…

A: Introduction Antibiotics are antimicrobial substances that are effective against microorganisms.…

Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…

A: Escherichia is a gram-negative bacterium, which under the microscope is shaped like a rod with a…

Q: In the petri dish in Figure 1, the bacteria Esherichia coli (E. coli) was grown and treated with…

A: The disk diffusion technique is used to estimate the susceptibility of the organism to a particular…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: Which one of the following is NOT considered a biohazard? old bacterial cultures slides…

A: Biohazard is defined as any biological substance that can pose or cause a threat and harm to biotic…

Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…

A: Culture media are an integral part of microbiology lab where bacteria can be grown on different…

Q: Why would you want to limit the number of times you passage a cell culture line?

A: Cell culture is the process through which cells are cultivated under controlled conditions,…

Q: The image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification…

A: Bacteria belongs to Kingdom Monera and are prokaryotic microorganisms that are generally…

Q: In which phase would you expect to find the most endospores in a bacillus cell culture? pick one…

A: Logistic curve: a sigmoid curve that grows exponentially for small values of the variable and…

Q: PART B Re-watch the video and explain why the following steps are necessary: Why use tweezers to put…

A: Please note as per the site regulations, external links are not watched. Hence, part A cannot be…

Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…

A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…

Q: You are incubating cells for 3 hours. The initial number of cells present in the sample were 102.…

A: Generation time is the time interval required for cells or a population to divide. Generation time…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: Introduction Magnification refers to enlarging something's apparent size rather than its true size.…

Q: a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a…

A: The staining is a very important process in microbiology that is used to identify specific…

Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…

A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…

Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…

A: Microscopy is the technique which is used to magnify small microorganisms and small specimens . It…

Q: You have a microscope with a resolution of 0.5 um. Your bacteria on your sample are 0.3 um apart on…

A: The light microscope is a versatile instrument used to study a wide variety of types of specimens…

Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…

A: The gram staining procedure that is used for staining and visualizing the bacteria. The bacteria are…

Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…

A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: A compound microscope is used by microbiologists to magnify microscopic samples. It uses a…

Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…

A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…

Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…

A: In microbiology the bacterial culture plate is used for observing the growth of the microorganisms.…

Q: You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and…

A: For calculating the number of bacteria or microorganisms within a sample we usually used the serial…

Q: It is called a "Negative Stain" because the stain has a negative charge, and is therefore: repelled…

A: An acid dye has negative charge hence are able to bind to the cell structures that are positively…

Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…

A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…

Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Energy is obtained during respiration process. The chemical equation is C6H12O6 + 6O2 → 6CO2 + 6H2O…

Q: In a new batch of culture medium, a group of sub-lethally wounded bacterial cells were transferred.…

A: Lag Phase- In this phase bacteria are still adapting themselves to the culture and are maturing with…

Q: Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram…

A: The Gram staining procedure in bacteria is depends on the chemical properties of cell wall. In Gram…

Q: If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many…

A: Introduction: CFU's: Colony-forming units are used to estimate the concentration of microorganisms…

Q: The image above was taken using a 40x objective lens in our MoCell lab with 2.5 calibration factor.…

A: A microscope is an instrument that can be used to observe the small objects like, cells. The image…

Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of
the old culture would you add to the new media?

Expert Solution

Step 1

Introduction

Dilution is the process of lowering the concentration of a solute in a solution by simply adding more solvent to the solution, such as water. Diluting a solution entails adding more solvent without adding more solute.

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?
To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volume
Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are counted. Answer the question below. there were _____cells in the 1 mL of culture that was spread on the plate. If the culture was diluted 1:100 prior to plating, how many cells were there per mL in the original culture? _________
You have a culture of cells at 3.5 x 108 cells/ml and you want to prepare a fresh 5 ml culture with a starting density of 1 x 104 cells/ml. What volume of the old culture do you add to the new 5 ml culture?
If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culture
In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?
You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?
You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?