You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene

You have cloned the protein coding region of the porin gene from a phage into a bacterial cell for a biotech company who wants to use the porin protein in phage therapy (the porin protein forms pores in the bacterial membrane). However, you notice that the porin gene you have cloned is not translated in the bacterial cells and as a result you cannot isolate the porin protein from those cells. What did you do wrong? O You forgot to include phage RNA polymerase, bacterial RNA polymerases cannot transcribe phage genes O You forgot to include the phage DNA polymerase, the gene cannot be replicated in the bacterial cell O You did not include the Shine and Dalgarno region for this gene

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...

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