You made a pH 9.0 buffer solution at 25° C by mixing NaOH and histidine (HisH) to give a solution that is 0.200 M in total concentration of histidine. Deprotonation constants and enthalpies (heats) of deprotonation at 25°C Compound Deprotonation pK A,H° (kJ mol=1) H3NRH+COOH→+H3NRH†COO- +H+ H3NRH†COO→+H3NRCO0 +H+ 1.80 Histidine 6.04 29.9 H3NRCOO¯→H2NRCOO¯ +H+ 9.33 46.6 Water H20¬OH¯ +H+ 14.00 55.82

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calculate the concentration of Na+ , H+ ,OH-. at 45 °C

You made a pH 9.0 buffer solution at 25° C by mixing NaOH and histidine (HisH) to give a solution that is 0.200 M in total concentration of histidine.
Deprotonation constants and enthalpies (heats) of deprotonation at 25°C
Compound Deprotonation
pK A,H° (kJ mol-1)
+H3NRH+COOH→+H3NRH†COO¯ +H+ | 1.80
Histidine
+H3NRH CO0¯→*H3NRCO0¯ +H+
6.04
29.9
+H3NRCOO--→H2NRCOO¯ +H+
9.33
46.6
Water
H2O→OH- +H+
14.00
55.82
Transcribed Image Text:You made a pH 9.0 buffer solution at 25° C by mixing NaOH and histidine (HisH) to give a solution that is 0.200 M in total concentration of histidine. Deprotonation constants and enthalpies (heats) of deprotonation at 25°C Compound Deprotonation pK A,H° (kJ mol-1) +H3NRH+COOH→+H3NRH†COO¯ +H+ | 1.80 Histidine +H3NRH CO0¯→*H3NRCO0¯ +H+ 6.04 29.9 +H3NRCOO--→H2NRCOO¯ +H+ 9.33 46.6 Water H2O→OH- +H+ 14.00 55.82
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