Chapter 1.8, Problem 2.2CS

In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either.  Materials: 200-ml Erlenmeyer flask                       Stove 500-ml Erlenmeyer flask                       Autoclave 10-mL graduated cylinder                     Analytical balance 100-ml graduated cylinder                    pH meter Spatula                                              Stirring rod 100-mL beaker                                   Test tubes Distilled water                                     Petri dish Stirring rod                                         Alcohol lamp Glass dropper

Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity?  When can gelatin be used in culture media preparation?  How does cotton plug prevent contamination of the medium?  Why should caps of glassware be loosened first before sterilization?

What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).