Aquaculture Science
3rd Edition
ISBN: 9781133558347
Author: Parker
Publisher: Cengage
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Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope.
At 100X you are unable to see the diatom. Explain why.
Explain what correction (s) should be made to allow you to see the diatom using the 100X lens.
After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred.
The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution.
Given the formula OCD = CFU/V x D, what as the Original Colony Density?
witj the following imformation create figures and explain the results
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