Foundations in Microbiology
Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 3.L2, Problem 1CT

A certain medium has the following composition:

Chapter 3.L2, Problem 1CT, A certain medium has the following composition: a. Tell what chemical category this medium belongs

a. Tell what chemical category this medium belongs to, and explain why this is true.

b. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium?

Expert Solution & Answer
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Summary Introduction

To analyze:

  1. The chemical category of the medium.
  2. Way of converting Staphylococcus medium into a nonsynthetic medium.

Introduction:

Culturing microorganisms out of their natural environments in pure form in the laboratory has been made possible only with the development of artificial medium or media. The role of media in the culture, identification, and mass production of microbes is remarkable. Based on the chemical composition there are two types of media: synthetic media with a chemically defined composition are used in research and cell culture and nonsynthetic, or complex, media with at least one component that is not chemically definable provide a rich mixture of nutrients for microbes with complex nutritional needs.

Explanation of Solution

  1. The medium mentioned here is a complex or nonsynthetic medium. The components yeast extract and peptone are complex organic compounds derived from yeasts and milk or meat. They cannot be chemically defined; hence, make the medium complex or nonsynthetic.
  2. The medium tabulated in table 3.6A is a synthetic medium with components that are chemically defined. The medium lists amino acids, vitamins, and salts in specific quantities, which can be replaced with organic compounds, to convert the medium into nonsynthetic medium. The brain-heart extract and peptone extract replace the dominant substances, however in unknown quantities, satisfying the specific nutrient requirements of Staphylococcus.
Conclusion

The medium in question is a complex or nonsynthetic medium that cannot be put into a single chemical class.

The medium in table 3.6 can be converted to a nonsynthetic medium by replacing the amino acids, vitamins, and salts with organic compounds and extracts (brain-heart extract) that will satisfy the nutrient needs of Staphylococcus.

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Chapter 3 Solutions

Foundations in Microbiology

Ch. 3.2 - Prob. 3CYPCh. 3.2 - Prob. 4CYPCh. 3.2 - Prob. 5CYPCh. 3.2 - Prob. 6CYPCh. 3.2 - Prob. 7CYPCh. 3.2 - Prob. 8CYPCh. 3.2 - Prob. 9CYPCh. 3.2 - Compare the way that the image is formed in the...Ch. 3.3 - Prob. 9ELOCh. 3.3 - Define dyes and describe the basic chemistry...Ch. 3.3 - Prob. 11ELOCh. 3.3 - Distinguish between simple, differential, and...Ch. 3.3 - Describe the process of Gram staining and how its...Ch. 3.3 - Prob. 11CYPCh. 3.3 - Explain what happens in positive staining to cause...Ch. 3.3 - Prob. 13CYPCh. 3.3 - For a stain to be considered differential, what...Ch. 3.3 - Prob. 15CYPCh. 3.4 - Prob. 14ELOCh. 3.4 - Prob. 15ELOCh. 3.4 - Explain what an isolated colony is and indicate...Ch. 3.4 - Differentiate between a pure culture, subculture,...Ch. 3.4 - What kinds of data are collected during...Ch. 3.4 - Prob. 19ELOCh. 3.4 - Prob. 16CYPCh. 3.4 - Prob. 17CYPCh. 3.4 - Prob. 18CYPCh. 3.4 - Compare and contrast three common laboratory...Ch. 3.4 - Describe how an isolated colony forms.Ch. 3.4 - Prob. 21CYPCh. 3.5 - Prob. 20ELOCh. 3.5 - Name the three general categories of media, based...Ch. 3.5 - Compare and contrast liquid, solid, and semisolid...Ch. 3.5 - Prob. 23ELOCh. 3.5 - Prob. 24ELOCh. 3.5 - Identify the qualities of enriched, selective, and...Ch. 3.5 - Explain what it means to say that microorganisms...Ch. 3.5 - Describe live media and the circumstances that...Ch. 3.5 - Describe the main purposes of media, and compare...Ch. 3.5 - Differentiate among the ingredients and functions...Ch. 3.5 - Explain the two principal functions of dyes in...Ch. 3.5 - Why are some bacteria difficult to grow in the...Ch. 3.5 - What conditions are necessary to cultivate viruses...Ch. 3.L1 - Prob. 1MCQCh. 3.L1 - Prob. 2MCQCh. 3.L1 - Prob. 3MCQCh. 3.L1 - Prob. 4MCQCh. 3.L1 - Prob. 5MCQCh. 3.L1 - Prob. 6MCQCh. 3.L1 - Resolution is _____________ with a longer...Ch. 3.L1 - Prob. 8MCQCh. 3.L1 - Prob. 9MCQCh. 3.L1 - The specimen for an electron microscope is always...Ch. 3.L1 - Motility is best observed with a a. hanging drop...Ch. 3.L1 - Prob. 12MCQCh. 3.L1 - Prob. 13MCQCh. 3.L1 - Prob. 14MCQCh. 3.L1 - What type of medium is used to maintain and...Ch. 3.L1 - Prob. 16MCQCh. 3.L1 - Multiple Matching. For each type of medium, select...Ch. 3.L1 - Prob. 1CSRCh. 3.L1 - Prob. 2CSRCh. 3.L1 - Prob. 3CSRCh. 3.L1 - Prob. 1WCCh. 3.L1 - Prob. 2WCCh. 3.L1 - Prob. 3WCCh. 3.L1 - Describe the steps of the Gram stain, and explain...Ch. 3.L1 - Describe the steps you would take to isolate,...Ch. 3.L1 - Prob. 6WCCh. 3.L1 - Evaluate the following preparations in terms of...Ch. 3.L2 - A certain medium has the following composition: a....Ch. 3.L2 - a. Name four categories that blood agar fits. b....Ch. 3.L2 - Prob. 3CTCh. 3.L2 - Go back to section 1.2 and observe the six...Ch. 3.L2 - Prob. 5CTCh. 3.L2 - Examine figure 3.10a, b (shown here). If you...Ch. 3.L2 - Prob. 2VC
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