Biomedical Instrumentation Systems
1st Edition
ISBN: 9781133478294
Author: Chatterjee
Publisher: Cengage
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A scanner tube is used in STM to precisely control the tip/sample position. What materialis in general the scanner tube made of and what is its working mechanism?
Concentration [M]
Absorbance
Solution 1
86.29
0.64
Solution 2
51.986
0.29
….
Fig 1: [graph of the calibration curve, make sure to show: function, R2, 0 intercept]
Three factors were studied: reactor length, carrier flow rate, and sample volume, with the high and low values summarized in the following table.
factor
high (+1) level
low (–1) level
X: reactor length
1.3 cm
2.0 cm
Y: carrier flow rate
1.6 mL/min
2.2 mL/min
Z: sample volume
100 μL
150μL
The authors determined the optimum response based on the greatest sensitivity, as determined by the change in potential for the potentiometric detector. The following table summarizes their optimization results.
run
X*
Y*
Z*
ΔE (mV)
1
–1
–1
–1
37.45
2
+1
–1
–1
31.70
3
–1
+1
–1
32.10
4
+1
+1
–1
27.30
5
–1
–1
+1
39.85
6
+1
–1
+1
32.85
7
–1
+1
+1
35.00
8
+1
+1
+1
32.15
Which factor has the greatest effect on sensitivity (change in potential at the detector)?
Which set of conditions optimizes the detection sensitivity?
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