Agar

The microbe that I received was on plate B section 5. I began the project by creating pure cultures of my microbe on a sterile nutrient agar plate, a nutrient agar slant and an agar broth by using Alderson’s procedure for aseptic techniques (Alderson, 2015, p. 53). After inoculating the media with my microbe, I incubated them at 37°C for 48 hours to allow for growth. After 48 hours, utilizing Alderson’s technique for making smears from solid cultures I created 3 smears (Alderson, 2015, pp. 93-94)

Introduction Avibacterium paragallinarum Avibacterium paragallinarum, previously known as Haemophilus paragallinarum, is a pathogenic bacteria that cause infectious coryza, an acute respiratory diseases that associate with substantial losses in poultry industry worldwide (Blackall et al., 2005). Early descriptions believed the pathogen of infectious coryza was Haemophilus gallinarum, which requires hemin and NADH as compulsory growth factors. In 1960s, studies on bacteria isolates recovered from

was the original phage suspension and from there we diluted it three more times in 9-ml dilution blank test tubes. Once the test tubes were inoculated .5-ml of each dilution was placed in soft agar tubes quickly, so that it would not solidify, and then were poured on the trypticase-soy agar plates. The TS agar plates were left to incubate for 6-10 hours. Once done incubating each plate was then counted to see how many plaques had formed on the bacteria lawn. Results The results of the plaque assay

One may wonder why research is important or why it is important to know how the things around came to be, exist, or interact with one another, particularly with respect to the microbial world. Knowledge that research provides is critical to the understanding of the known world around us. Experiments are what have helped to provide answers to questions, thus fostering knowledge, which allows us to enjoy the lives we live today. Research via experimentation is a critical component as the information

properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values

Introduction The purpose of this experiment was to allow each student to perform certain procedures and utilize the skills accumulated over the semester in order to interpret and identify two unknown organisms within a mixed culture. Each student was given a mixed culture containing one Gram negative organism and one Gram positive organism. I received unknown mixed culture #15. The possible Gram negative organisms within the mixed culture include: Enterobacter aerogenes, Pseudomonas aeruginosa,

MacConkey Agar plates -Glass hockey stick -Jar of alcohol -Bunsen burner -Flint striker -Microscope slide & Glass coverslips Method: 1. Make a wet mount (Ex 12) of a small sample of the alfalfa water. Examine at 1000X total magnification using high contrast. This will illustrate how many organisms, both motile and non-motile, are present in the sample. 2. Make 10-old dilutions according to the diagram (Fig. 16.2). 3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate

A 10-Fold Dilution Series process was necessary to examine the bacteria further. The materials needed included: one Bunsen burner, one inoculation loop, one BIC ® Multi-Purpose lighter, three sterile LB Agar Petri dishes, one bottle of sterile saline solution, one pipette gun, one canister full of sterile glass pipettes, a medium sized test tube track, and one plastic tub of medium glass test tubes. Next, one participant’s 10-Fold Dilution series was performed and three lines of ten medium test tubes

will a vortex. We then piped 1ml of the first dilution into the second dilution and mixed thoroughly with the vortex. This process was repeated with remainder of the dilutions.. Streak plates were prepared in petri dishes filled with premade nutrient agar and 100µl of the third, fourth and fifth dilutions. We streaked an additional

technique as the experiment is conducted. The benefits of the streaking technique is when a cultures has multiple species they are able to be more easily identified once they have been isolated. This experiment is much like the experiments completed on an agar plate but on one a much larger scale and where techniques