Formazan

Electrospinning is a convenient method for fabricating various nanofibrous scaffolds for biomedical applications. In electrospinning process, a conductive collector device plays a critical role in determining the shape and the structure of the nanofibrous scaffold; however, the preparation of the collector is often complex. In this study, a novel, flexible, and inexpensive approach based on pencil-on-paper method was developed for preparing collectors used in electrospinning. Graphite is a conductive

After the hour incubation with MTT and the addition of DMSO, the blue DMSO-formazan solutions from each well was placed in a Kimex tube and run through a spectrophotometer. Each tube was placed in the spectrophotometer and had its absorbance value recorded three times. At the end, the average of each concentration was calculated. Table 1 shows that as the concentration of aspartame increased, the absorbance value decreased compared to the control (0mM). The highest absorbance value was seen at our

Introduction The number of cells is closely regulated by the rate of cell division and the rate of cell death. When cells become unneeded, they activate an intracellular death program and commit suicide. This process is often known as apoptosis. Cells die for numerous reasons. For instance, cell death regulates cell numbers and can adjust the number of cells needed for a particular function. When a structure a cell forms is no longer needed, the cell will die. Cells can become damaged or stressed

SPECIFIC AIM 1: Assess efficacy for four chemical lead compounds (SMYD2 Inhibitors) to halt cell cycle progression and induce apoptosis in CML and MLL cell lines. The purpose of Specific Aim 1 is to assess the level of efficacy for these inhibitors (AZ505, LLY-507, A-893, BAY-598) for CML and MLL cell lines to better characterize the molecular target indications of SMYD2 inhibition. Introduction and significance: In previous study, a knockdown of SMYD2 led to an increase in cell-cycle arrest and

succinate-dehydrogenase, cleaves the tetrazolium ring, converting the MTT to an insoluble purple formazan. Therefore, the amount of formazan produced is directly proportional to the number of viable cells. After 48 h of incubation, 15
L of MTT (5 mg/mL) in phosphate buffered saline (PBS) was added to each well and incubated at 37˚C for 4 h. The medium with MTT was then flicked off and the formed formazan crystals were solubilized in 100
l of DMSO and then measured the absorbance at 570 nm using micro

bromide (MTT) by mitochondrial succinate dehydrogenase. The MTT starts as yellow but as it enters the cells and passes into the mitochondria where it is reduced and changes in colour to a dark purple formazan product. The cells are then solubilised with an organic solvent and released, solubilised formazan reagent is measured using a spectrometer (Cree, 2011). Some errors that may occur while using this assay are that uneven evaporation of culture fluid in the wells may cause erroneous results. The MTT

Cancer is one of the most prevalent groups of disorders in the population in many countries worldwide. Epidemiologic studies have indicated that most human cancers are originally caused by environmental exposure to genotoxic agents (Doll et al., 1981). With respect to global human health hazard, arsenic (As) is one of the most important environmental single substance toxicants. It is a metalloid element and exists in organic and inorganic forms (iAs). Later is considered as a class I human carcinogen

Human breast cancer MCF-7 cells were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were maintained in RPMI 1640 medium supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO2. Cell viability examination To evaluate the cytotoxic ability of WECU, MCF-7 cells were seeded in 6-well plates at a density of 2×104 cells per well. After incubating overnight, the cells were treated with

This experiment was conducted as per the BCHM 310 Laboratory Manual [3]. The first objective of this experiment was to analyze the purity of the invertase fractions collected during experiment 6, and to determine the molecular weight of LDH-H4, LDH-M4 and invertase subunits. This was accomplished using sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE). In this procedure, SDS, a negatively charged amphipathic molecule, was used to denature the proteins and to give each protein

succinate dehydrogenase and the fumerate involved in the reaction are both colourless so it is impossible to measure any changes using a spectrophotometer. Instead the absorbance of formazan is measured at 490nm. The formazan is produced by reacting the FADH2 from the SDH reaction with INT which a tetrazolium salt. The formazan produced is red in colour and when dissolved into the ethyl acetate can be extracted and then read on the spectrophotometer (Smith and McFeters, 1997). As