Formazan

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  • Electrospinning Essay

    814 Words  | 4 Pages

    Electrospinning is a convenient method for fabricating various nanofibrous scaffolds for biomedical applications. In electrospinning process, a conductive collector device plays a critical role in determining the shape and the structure of the nanofibrous scaffold; however, the preparation of the collector is often complex. In this study, a novel, flexible, and inexpensive approach based on pencil-on-paper method was developed for preparing collectors used in electrospinning. Graphite is a conductive

  • Aspartame Lab Report

    407 Words  | 2 Pages

    After the hour incubation with MTT and the addition of DMSO, the blue DMSO-formazan solutions from each well was placed in a Kimex tube and run through a spectrophotometer. Each tube was placed in the spectrophotometer and had its absorbance value recorded three times. At the end, the average of each concentration was calculated. Table 1 shows that as the concentration of aspartame increased, the absorbance value decreased compared to the control (0mM). The highest absorbance value was seen at our

  • Cell Viaability Lab Report

    1172 Words  | 5 Pages

    bromide (MTT) by mitochondrial succinate dehydrogenase. The MTT starts as yellow but as it enters the cells and passes into the mitochondria where it is reduced and changes in colour to a dark purple formazan product. The cells are then solubilised with an organic solvent and released, solubilised formazan reagent is measured using a spectrometer (Cree, 2011). Some errors that may occur while using this assay are that uneven evaporation of culture fluid in the wells may cause erroneous results. The MTT

  • Cancer : One Of The Most Adverse Effects Of Human Cancer

    1216 Words  | 5 Pages

    Cancer is one of the most prevalent groups of disorders in the population in many countries worldwide. Epidemiologic studies have indicated that most human cancers are originally caused by environmental exposure to genotoxic agents (Doll et al., 1981). With respect to global human health hazard, arsenic (As) is one of the most important environmental single substance toxicants. It is a metalloid element and exists in organic and inorganic forms (iAs). Later is considered as a class I human carcinogen

  • Invertase Lab Report

    1888 Words  | 8 Pages

    This experiment was conducted as per the BCHM 310 Laboratory Manual [3]. The first objective of this experiment was to analyze the purity of the invertase fractions collected during experiment 6, and to determine the molecular weight of LDH-H4, LDH-M4 and invertase subunits. This was accomplished using sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE). In this procedure, SDS, a negatively charged amphipathic molecule, was used to denature the proteins and to give each protein

  • Rationale And Disorcy For Cell Cycle Infection

    880 Words  | 4 Pages

    SPECIFIC AIM 1: Assess efficacy for four chemical lead compounds (SMYD2 Inhibitors) to halt cell cycle progression and induce apoptosis in CML and MLL cell lines. The purpose of Specific Aim 1 is to assess the level of efficacy for these inhibitors (AZ505, LLY-507, A-893, BAY-598) for CML and MLL cell lines to better characterize the molecular target indications of SMYD2 inhibition. Introduction and significance: In previous study, a knockdown of SMYD2 led to an increase in cell-cycle arrest and

  • Cell Viability Of MCF-7 Cells

    1401 Words  | 6 Pages

    Human breast cancer MCF-7 cells were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were maintained in RPMI 1640 medium supplemented with 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C in a humidified atmosphere with 5% CO2. Cell viability examination To evaluate the cytotoxic ability of WECU, MCF-7 cells were seeded in 6-well plates at a density of 2×104 cells per well. After incubating overnight, the cells were treated with

  • A Experiment On Rat Liver Cells

    1625 Words  | 7 Pages

    succinate dehydrogenase and the fumerate involved in the reaction are both colourless so it is impossible to measure any changes using a spectrophotometer. Instead the absorbance of formazan is measured at 490nm. The formazan is produced by reacting the FADH2 from the SDH reaction with INT which a tetrazolium salt. The formazan produced is red in colour and when dissolved into the ethyl acetate can be extracted and then read on the spectrophotometer (Smith and McFeters, 1997). As

  • The Effects Of A Putative Anti Cancer Drug On Hela Cells

    3121 Words  | 13 Pages

    Introduction Background According to the Office of National Statistics, in 2013, 29% of deaths in the UK were cancer related, making it the leading broad cause of death in the population. In the United States, 1 in 4 deaths are cancer related, and it is estimated that in a lifetime, there is 44% chance of contracting an invasive cancer if you are male, and a 38% chance if you are female  (Siegel et al. 2014). So, although our knowledge and education into different cancer mutagens such as UV radiation

  • Hepg2 / C3a Cells : The Choice Of Cell Lines

    1227 Words  | 5 Pages

    5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H as reported by the manufacturer's instructions (Sigma-Aldrich, USA ). The tetrazolium salt is cleaved to formazan by succinate- reductase, an enzyme that exists only in the mitochondrial respiratory chain and is active only in viable cells. Formazan production is proportional to the number of living cells in culture. Briefly, HepG2 / C3A and PLC / PRF / 5 were seeded at 104 cells per well in 96 well plates. At 80% confluence

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