Gram staining

wanted to use for this experiment I had to decide on E.coli bacteria which is gram negative and staphylococcus a gram positive bacterium. These were chosen because they are safe enough to grow in a college laboratory and were supplied by the technician. Gram negative bacteria has an outer membrane making it more resistant to antiseptics and antibiotics, it also makes it more fatal to the human host it is inhabiting. Whereas gram

Serial dilutions, also known as limiting dilution series, help to determine an estimation of how many phages/viruses are in a given culture (Zelterman et al., 2010). In this experiment we determined how many phages were in our given Salmonella typhimurium culture by doing three serial dilutions of an original phage suspension. This is important because it will allow us to get a rough estimation of how many phages where in our original sample and also provide us with knowledge on how to perform serial

Teaching People How to Grow, Extract, Harvest and Use Plant Products as Antimicrobial/Pharmaceutical Agents Plant Products as Antimicrobial Agents Abstract 25-50% of current pharmaceuticals are derived from plants Traditional healers used plants to cure infectious conditions Plants are rich in: Secondary metabolites they are not generally included in standard metabolic charts. found to have have antimicrobial properties. Tannins compounds that have a tendency to interact with aqueous solutions

Enterobacter aerogenes is a species of rod shaped gram negative bacterium in the family of enterobacteriaceae. It is a facultative anaerobe, meaning that it can grow in either the presence of oxygen or in the absence of oxygen. It also uses fermentation as the means of gathering energy that protect it from reative oxygen species, and allowing it to live in oxygenated environments. Enterobacter aerogenes is a nosocomial and pathogenic bacterium that cause opportunistic infections. The majority are

the growth of S. albus. The highest concentration of Dettol (100% - D) was predicted to be the most effective solution to kill and stop the growth of this particular bacteria. This can be justified as S. albus is gram-positive; easier bacteria to kill, developing resistance slower than a gram-negative. Therefore, the highest concentration of antibiotic should be – and was – the most successful in changing the pH levels, and killing S. albus. Through discussion and analysis of the results collected through

large intestines of humans and animals. Coliform bacteria can also be found in water and in soul. They are gram-negative bacteria’s, meaning that they have a peptidoglycan layer and another layer of the cell wall. These bacteria are also a rode shape now as bacilli. As we all know or should know by now, gram negative cells have a equine structure to their cell wall other cells like acid fast and gram positive do not have. They have a hairy looking type of structure projecting from the outer layer of the

Biphosphonate used when alkaline phosphates’ is at least twice the upper limit of normal. Edironate slows accelerated bone turnover and is accompanied by symptomatic improvement, less bone pain, and decrease in bone fractures. Initial dose is 5 to 10 mg/kg daily for up to but not exceeding 6 months or 11 to 20 mg/kg not to exceed 3 months. Retreatment for relapse is acceptable only after more than 90 drug free days. Patient with Paget’s disease benefit from supplemental calcium and vitamin D if

An evaluation of the antioxidant status and antimicrobial activity of the methanol extract of Ocimum gratissimum. Sajini Souda1*, George Saramma1, Irene Goercke2, Kelvin Chabaesele3,Naledi Mannathoko1. 1. Faculty of Medicine, University of Botswana, Gaborone, Botswana. 2. School of Allied Health Sciences, Faculty of Health Sciences, University of Botswana, Gaborone, Botswana. 3. Department of Biological Sciences, University of Botswana, Gaborone, Botswana *Corresponding author E-mail address:

unique One way to tell the difference is to see if the bacteria is gram positive or gram negative. Gram-Positive bacteria contains peptidoglycan (which is a polymer of amino acids and sugars), while Gram-Negative Bacteria does not have as much. (Holbrook, 24) Because Gram-Positive contain peptidoglycan, they do not form string in the KOH test. The opposite is true for Gram-Negative- it will form a string due to its chemical makeup. The Gram-Positive bacteria uses the peptidoglycan to act as a wall, so

of bacteria that contain peptidoglycan, Gram-positive and Gram- negative. Gram positive bacteria will stain dark purple due to their thick peptidoglycan cell wall, while Gram negative will stain pink due to their thin peptidoglycan cell wall. Many Gram positive and negative bacteria are considered pathogens because they are capable of causing disease in humans. In order to treat properly individuals who are infected with a pathogenic Gram positive or Gram negative bacteria, the identity of the bacteria