Taq polymerase

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  • Abstract. Taq Polymerase Is Essential In Polymerase Chain

    1446 Words  | 6 Pages

    ABSTRACT Taq Polymerase is essential in Polymerase Chain Reaction(PCR) experiments to obtain a PCR amplification of an unknown gene. The unknown gene is then ligated into a vector plasmid, which is placed in a bacterium Escherichia Coli to transform and multiply. Ultimately, identification and characterization of the unknown gene is done using electrophoreses and gel imaging. Cloning techniques such as the one performed have been used for many years to isolate genes from a variety of species. Isolation

  • Seedling Salinity Tolerance Report

    748 Words  | 3 Pages

    using SSR markers. The PCR reactions were performed with 15 μl final volume containing 50 ng (2 µl) of genomic DNA, 1.5 µl of 10X buffer containing 25mM MgCl2, 1.5 µl of 2.5 mM dNTP mix, 1 µl each of forward and reverse primers (5 mM), and 1U of Taq polymerase. The

  • Essay about Silver’s Remaking Eden and the Silver Screen

    1193 Words  | 5 Pages

    Silver’s Remaking Eden and the Silver Screen In Remaking Eden, Lee M. Silver asks three central questions: Who controls life? What counts as life? And what will human life look like in the future? The question Silver does not ask is whether or not human life as we now know and define it will change. Silver sees the advance of genetic engineering as inevitable, due to consumer demand for it as a technology and the unrelenting curiosity of scientists. Power resides in science, according to Silver

  • Essay on Using PCR and Gel Electrophoresis to Determine Genotype

    583 Words  | 3 Pages

    Using PCR and Gel Electrophoresis to Determine Genotype In certain situations, it is necessary to identify DNA retreived from a sample. When there is a small sample in need of identification, Polymerase Chain Reactions are used to multiply the DNA in the sample in to many identical samples. The DNA retrieved from the reaction can then be imported into an aparatus using gel electrophoresis to compare the sample of DNA to other samples. In our experiment we learned the how to replicate tiny

  • Lab Report : Bacillus Subtilus Using A Polymerase Chain Reaction

    1903 Words  | 8 Pages

    The purpose of this experiment was to amplify the α-amylase gene in Bacillus subtilus using a polymerase chain reaction. Bacillus subtilus is a gram-positive bacterium that resides in soil and the gastrointestinal tract of animals and primarily uses aerobic respiration for survival (Yu et al., 2015). Due to it’s ability to take part in the fermentation process, B. subtilus can produce large amounts of the α-amylase enzyme which is responsible for catalyzing the digestion of polysaccharides, such

  • Polymerase Chain Reaction: Lab Technique

    407 Words  | 2 Pages

    PCR (Polymerase Chain Reaction) is a laboratory technique that takes specific fragments of DNA and amplifies them using primers and a polymerase that can withstand high temperatures. The materials needed to complete this experiment include the DNA fragments that are to be amplified, two primers (one to attach to the top strand of the DNA fragment of interest and one to attach to the bottom strand on the other end), Taq DNA polymerase, dNTP mix, MgCl2, PCR buffer and the PCR machine. Primers are necessary

  • Polymerase Chain Reaction

    402 Words  | 2 Pages

    Polymerase chain reaction, also known as PCR, is a technique to make multiple copies of a specific DNA region. PCR relies on a thermostable DNA polymerase and requires DNA primers for the specific regions of DNA that is being used. The main goal of PCR is to make enough of the specific DNA, in that region, that can be analyzed for many different reasons. For example, having enough of the DNA amount for forensic scientist would help them match crime scene DNA to the actual suspect. PCR requires

  • Basic Principles Of The Polymerase Chain Reaction ( PCR )

    1080 Words  | 5 Pages

    the polymerase chain reaction (PCR). PCR is biochemical research technique developed in 1985 by Kary Mullins. It is based on the principle of amplifying DNA through a series of cycling reactions with varying temperatures. PCR uses the ability of thermostable polymerase enzymes to synthesize a new complementary DNA strand to a template strand. b. What are the essential components of a PCR reaction? The essential components of PCR reactions are: i. Taq polymerase: A thermostable DNA polymerase isolated

  • Definition Of Binary Expression Systems For Regulating Gene Expression

    1290 Words  | 6 Pages

    and the storage plasmid and tack those at the beginning of the primer). b. Isolate genomic DNA from WT animal (want WT version of the gene) to be used as a template c. Run PCR reaction with genomic DNA as template + For and Rev primers, dNTPs, polymerase (Taq

  • Genealogy: The First Step In Sequencing My DNA

    1039 Words  | 5 Pages

    tell me what my DNA sequence is. Because there is the potential for the sample that I sent to not have a great deal of DNA available, polymerase chain reaction may be used to amplify the DNA to get a better sample to analyze. In polymerase chain reaction, they will take the small sample of DNA that I have provided along with two oligonucleotide primers, Taq polymerase, and four deoxynucleotide triphosphate and amplify the sample of DNA. In the first step, the DNA is heated to 95 degrees Celsius in

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