Taq polymerase

repetitive element such as the “Alu sequence” is present in the PV92 region of chromosome 16 of my DNA. I will then gather the data from my Biology Lab Class and compare it with those of the United States. To perform this experiment, the method of Polymerase Chain Reaction (PCR) will be used to amplify the DNA. The purpose of using PCR is to create enough mass in order for it to run on the agarose gel electrophoresis. Performing this

In this article examples are going to be discussed of the application of molecular biology in the field of plant ecology, what molecular biology is as well as what plant ecology is. Plant ecology is one of the branches in the scientific field of ecology that mainly focus on plant population and their surroundings (McMahon, 2016). Plant ecologists also look at other factors that have an impact on the plants and their environment. According to the MIT Department of Biology, plant molecular biology

Introduction HL-60 cells, or Human Leukemia cells, are known for their special characteristics such as immortality, as well as their ability to differentiate into various hematopoietic cell types, such as monocytes and granulocytes (1). Aside from these known characteristics, researchers have taken an interest in HL-60 cells because of their differentiation on behalf of numerous chemical inducers (1). HL-60 cells have been discovered to differentiate into granulocytes upon treatment of Dimethyl

There is very little customers can do to ensure that they are purchasing the fish they are paying for. Producers and restaurants owners have a tendency to mislabel their products for their own reasons. The main reason is yet to be determined but there are researchers who have made it their goal to resolve this dispute and show customers what they are truly buying. Too Chin Chin et al. attempt to utilize the DNA barcoding in the fish segment, to assess the frequency of fish mislabeling on the Malaysian

PCR Gel Electrophoresis Introduction Sickle cell anaemia is caused by a Single Nucleotide Polymorphism (SNP). SNPs are the most common type of genetic variation, with each SNP representing a difference in a nucleotide. SNPs occur normally throughout a person’s DNA. Most commonly, they are usually found in non-coding regions and have no effect on health. When a SNP occurs in a coding region, it may play a direct role in disease by affecting the gene’s function. This SNP for sickle cell anaemia produces

The polymerase chain reaction or PCR for short can be used to create many copies of DNA. This allows the DNA to then be visualized using a dye like ethidium bromide after gel electrophoresis. The process has been refined over the years, however the basic steps are similar. The first is to denature dsDNA through heating to ~96 °C. This separates the two strands of DNA. The exact temperature to be used can be calculated with Tm = 4oC x (no. of G & C) + 2oC x (no. of A & T). Tm is the melting point

INTRODUCTION: Modern cattle breeding increasingly involves the widespread use of advanced reproductive technologies, including artificial insemination (AI) and multiple ovulation embryo transfer (MOET), frequently with the germplasm of elite international sires and dams selected through individual and progeny performance recording schemes. These technologies facilitate the rapid worldwide multiplication of offspring from elite animals to assist the propagation of genes that improve milk yield, meat

(Hartvig, 2015). It is effective in differentiating between phenotypically similar species and is applicable to all organisms of life (Dudu, 2016). For DNA barcoding, the DNA is isolated from a sample and standard genetic markers are amplified by Polymerase Chain Reaction (PCR). A polymorphism is differences in DNA sequence that accumulate over time (Albert, 2011). The main source of mutations occurs during DNA replication and, thus mutations can be inherited. When the frequency of the mutation increases

2. Materials and methods 2.1. Parasites and mice The B. bovis Texas strain (Hines et al., 1992) and the B. bigemina Argentina strain (Hotzel et al., 1997) were maintained in bovine red blood cells (RBCs) in in vitro culture (Munkhjargal et al., 2012). Infected red blood cells (iRBCs) were harvested at 5e6% parasitemia, washed three times with phosphate buffered saline (PBS), and then stored at 80 C until use. The Munich strain of B. microti was maintained in BALB/c mice by serial passaging. Infections

150mM NaCl, 5mM KCl, and 2mM MgCl2) and SELEX 2 buffer (10mM HEPES, 150mM NaCl, 5mM KCl). The composition of the PCR mix was as follows: 10% 10X buffer, .5% Taq, 1% P1, 1% P2, 2% dTNP, and 80.5% water. All gels were run in .5X TBE buffer and 3% agarose gel. Columns used: Micro Bio-Spin™ Chromatography Columns. PCR polymerase used was Choice Taq from Denville Scientific. DNTP is also from Denville Scientific. Streptavidin Beads are from Sigma Aldrich. 4.2 The SELEX Process The general steps of the