Urease

Although they can’t be seen to the naked eye, it is vital that we comprehend the important role microorganisms play in our everyday life. These organisms’ importance goes as far back as causing the start of evolution on the planet Earth, which in turn led to the wide variety of species that we can find in different parts of the world nowadays and even to the existence of us. Having in depth knowledge of microbiology extends beyond the past, and into the influence it can have on our future. The ubiquitous

| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing

I obtained Unknown #518 during the initial project week. To begin our discovery for our unknown, we were instructed by Professor Do to carry out a streak technique on a MacConkey’s Plate for general growth. We also had to do a streak technique onto a Nutrient Agar Plate in order to separate the Gram-Positive microbe from the desired Gram-Negative microbe that we were trying to identify. Before the streak technique was performed, the sample was “vortex” to mix solution, then a loop was sterilized

For the final lab activity, I randomly selected a test tube containing an unknown bacterial microorganism #8 which was in liquid broth form. The purpose of this lab is to utilize an assortment of lab techniques that we have learned throughout the semester and understand how the differences in results could help us in the identification process of an unknown bacteria. The case study corresponding with my unknown is an 86-year-old male nursing home patient who has been suffering from fever, chills

determine the identification of a gram negative unknown. The seven biochemical tests that were tested for in this experiment were: Sulfur Indole Motility (SIM), Gelatin, Methyl Red (MR), Voges-Proskauer (VP), Citrate, Triple Sugar Iron Agar (TSIA), and Urease. These tests were inoculated and put inside a hot room of 37◦ C for different lengths of times; 24 hours, 48 hours, or eight days. Using these tests, allowed for determining whether the unknown bacterium had the ability to produce H2S, gas, ferment

positive and gram negative bacteria. This is an important step because it directs what the next tests will be. My Gram Stain on sample #12 showed that the bacteria was gram negative, however, after receiving the results of the OF glucose, H2S, Citrate, Urease and Motility tests, it was apparent that my Gram Stain was contaminated. I then performed a catalase test which came back negative, so I ordered a Bacitracin disc, Optochin disc and a CAMP test which had to be incubated overnight. After receipt

ABSTRACT The sole purpose of this project was to identify an unknown bacteria sample #7. Many tests were carried out to determine what this unknown was. Aside from a microbiology lab, understanding and identifying various organisms are important in disease processes, pharmaceutical arenas, and even in the industrial field. Proper lab techniques, including aseptic technique were used throughout the process of identification. INTRODUCTION There are three domains of organisms, which are Bacteria

In biochemical test, which is base on the hydrolytic catalytic enzyme that show their main similarities between Halo bacterium Salinarum and Haloferax volcanii that focus from its result which show to be as negative that is turn out to be as gelatin and whether it can able to substantiate but it’s not possible as is too difficult to melt it. Concern from its illustration that it is been demonstrate on how negative effect is focus also from casein hydrolysis and from Tween 80 and Tween 20. But in

9. Carbohydrate Fermentation Materials: Inoculating loop, Bunsen burner, glucose broths, sucrose broths, lactose broths, unknown 413. Procedure: Aseptic technique was carried out to prepare working area. Each tube was labeled with their names respectively. Unknown 413 was inoculated in the glucose, lactose, and sucrose broth. Each tube was incubated at 37℃ for at least 48 hours. 10. Identifying the unknown using complex and differential media. Materials: One test tube of Unknown 413, loop, Bunsen

order additional laboratory tests specifically used for Gram-negative organisms. The tests included: 1. Oxidization/Fermentation Glucose Test 2. Citrate Utilization of Sodium Citrate Test 3. SIM Agar – production of H2S, Indole and Motility Test 4. Urease Test Below is the listing of the test results I obtained and the purpose