Microbiology Exam #1 Study Guide
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CH. 1
1. Define the following terms: microorganism (microbe), pathogen, and infectious disease. -Microorganism: Bacteria, archaea, algae, protozoa, helminths, fungi, and viruses. -Pathogen: A disease causing microorganism. -Infectious Disease: Diseases caused by a pathogen.
2. Explain how an infectious disease is different from a non-infectious disease. -An infectious disease is caused by a pathogen where as a non-infectious disease is caused by another factor. 3. Explain the Germ Theory of Disease and how it differs from older concepts of disease. -The Germ Theory of Disease states that infectious diseases are caused by microorganisms (microbes). Previous ones include the Miasma Theory of Disease (smelly odors cause disease) and spontaneous generation (life created from nonliving things). 4. Identify the specific contributions of scientists/ microbiologists discussed in class. -Hooke: 1
st
individual to view cells under microscope
-Leeuwenhoek: 1
st
person to view bacteria, “animalcules”.
-Jenner: 1
st
smallpox vaccine.
-Semmelweis: Advocated handwashing. -Pasteur: Disproved spontaneous generation, supported Germ Theory of Disease.
-Lister: Introduced aseptic technique.
-Cohn: Discovered endospores.
-Koch: Verified Germ Theory of Disease, Koch’s postulates.
-Fleming: Discovered Penicillin, antibiotics available in nature. 5. Identify the five different pathogens. -Bacteria, Protozoa, Fungi, Helminths, and Viruses.
6. Identify where the five different pathogens belong on the tree of life. -Bacteria = prokaryote, Protozoa = eukaryotes, fungi = eukaryotes, helminths = eukaryotes, viruses= none, acellular
7. Compare and contrast prokaryotes and eukaryotes. -Prokaryotes: No nucleus, no organelles, single/circular chromosomes, 70S ribosomes, reproduce through binary fission. Their DNA is floating in the cytoplasm.
-Eukaryotes: Have nucleus and organelles, multiple chromosomes, 80S ribosomes, reproduce through mitosis/meiosis. Their DNA is housed in the nucleus. 8. Define human microbiome and normal microbiota. -Human microbiome is a broader term for all of the microbes present in our body (normal microbiota). This includes bacteria, archaea, fungi, protozoa, and viruses that do not cause harm and are sometimes beneficial. 9. Differentiate between the terms pathogen and normal microbiota. -Pathogens are disease causing microbes whereas normal microbiota is not harmful, they are normal in our bodies and can be beneficial sometimes.
10. Conclude whether a pathogen is prokaryotic or eukaryotic based on its physical characteristics. -Bacteria= Prokaryote
-Protozoa= Eukaryote
-Fungi=Eukaryote
-Helminth= Eukaryote
-Virus= Neither (acellular)
11. Describe taxonomic hierarchy. -Taxonomic hierarchy is a form used to classify living things starting from general to specific.
12. Differentiate between the terms “genus” and “species.” -Species is a type of genus, meaning it is a specific type of a broad form of genus. Genus is the first part of the binomial nomenclature and species is the second part.
13. Type out the genus and species name of a microbe in the correct format. -E. coli
14. Distinguish a virus from other pathogens. -Viruses are acellular and have a simple structure. They are mainly made up of two main structures, a protein shell and genetic material. They are neither alive or dead. CH. 3 Pt. 1
1. Recognize and name the shapes and arrangements of bacteria. -Cocci, bacillus, and spirillus. 1) Bacillus & Coccus
2) Diplobacilli & Diplococci
3+) Streptobacilli & Streptococci
4) Tetrad (cocci)
Grape (cocci)= Staphylococci
Spirilla (least to most spiral) = Vibrio, Spirillum, Spirochete
2. List the structures that all bacteria share in common. -Cell membrane, cytoplasm, ribosomes, cytoskeleton, 1 or a few chromosomes.
3. Describe and draw the structure of the cell membrane. -Phospholipid bilayer, phospholipids contain a hydrophilic head & hydrophobic tails.
4. Describe the function of the cell membrane. -Cell membrane regulates the passage of things in & out of the cell. Also, a site for chemical processes like ATP production.
5. Describe peptidoglycan. -Peptidoglycan is a repeating framework of long glycan chains cross-linked by short peptide (protein) fragments.
6. Describe, draw, and label the structure of a Gram positive and a Gram negative cell wall. -Gram (+): Thich cell wall made of peptidoglycan over the cell membrane. Teichoic & Lipoteichoic Acid. Acids add structural stability to wall. More resistant to physical stress.
-Gram (-): Outer membrane over thin peptidoglycan cell wall over cell membrane. Lipopolysaccharides & Porin Proteins. More resistant to chemical stress. 7. Compare and contrast, in detail, the Gram positive and Gram negative cell walls. -Gram positive cell wall contains a thick layer of peptidoglycan while the gram-negative cell wall
has a thin layer of peptidoglycan. Gram positive is more resistant to physical stress, gram negative is more resistant to chemical stress. 8. Explain how the bacterial cell membrane and the bacterial cell wall are distinct in structure and function. -The cell membrane functions in the regulation of materials passing through. Cell walls function in structural stability for the cell and determine shape. 9. Explain how the antibiotic penicillin affects the bacterial cell wall. -Penicillin disrupts the peptidoglycan in the cell wall by preventing the synthesis of peptide bridges that crosslink the glycan chains of peptidoglycan. This leads to the bacteria exploding from osmotic pressure. 10. Explain how the cell walls of Mycoplasma and Mycobacterium differ from Gram positive/ Gram negative cell walls. -Mycoplasma bacteria lack a cell wall, instead they have a membrane with sterols. Mycobacterium contain mycolic acid in their cell walls (acid-fast staining).
11. Describe the structure and function of a flagellum, axial filaments, fimbriae, pili, S layer, capsule and slime layer. -Flagellum: Tail-like structure made of flagellin that is used for motility. Can be on the outside or
inside (spirochetes). -Axial filaments: Internal flagella found only in spirochetes, wriggly movements.
-Fimbriae: Numerous bristle fibers made of pilin that help bacteria attach.
-Pili: Made of pilin, function in attachment and DNA transfer (can also transfer antibiotic resistance) to another bacteria.
-S layer: Only made under hostile environment, “armor.”
-Capsule: Type of glycocalyx, used for attachment and protection from phagocytosis. -Slime layer: Type of glycocalyx, protects against loss of water and nutrients.
12. Explain how an axial filament is different from a flagellum. -Axial filament is an internal flagellum that are unique to spirochetes, flagella are on the outside
and result in locomotion (360-degree spins).
13. Identify different flagellar arrangements. -Monotrichous: Single
-Lophotrichous: Tuft on one end of the cell (like hair)
-Amphitrichous: One or more on both ends of cell (like antennas)
-Peritrichous: Distributed all over cell surface
14. Compare and contrast fimbriae and pili in respect to structure and function. -Fimbriae and pili are both made of pilin. Fimbriae is mainly used for the attachment of the bacteria onto another surface, while a pilus is mainly used for the transfer of genetic
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