Dilutions Math in Excel

How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?

The doctor ordered Oxacillin 650mg every 6 hours.  The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show work

calculate the [PNP] in μM for each of these samples.

You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.

The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v?   I've used this with direct dilutions, how would I use this on serial dilutions?

In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?

You have prepared the following serial dilution using tryptone broth:d1: 0.1ml culture + 9.9 ml brothd2: 300µl d1 + 4.7 ml brothd3: 0.2ml d2 + 9800 µl broth1) Write your dilutions as a rational number AND in scientific format? What was your total dilution (in scientific notation)?2) What was the original concentration of your culture if you spread 0.1 ml of d3 and counted360 cfu?

The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?

An order was received for 750 mL of 7.5% dextrose solution using 5% D5W and 20% D20W.  How many parts of each dextrose solution is needed?(round answers to the nearest tenth)

3) With our 12.5 ug/mL solution, we will make serial dilutions with a dilution factor of 2. We want to make 1 mL of 1/2, 1/4, 1/8, 1/16, 1/32, and 1/64 dilutions. Draw a diagram describing how you will conduct the serial dilutions and insert it below. The 1/2 dilution (tube 2) will be made from the 12.5 ug/mL solution (tube 1). The 1/4 dilution (tube 3) will be made from the 1/2 dilution (tube 2L

How many CFU per ml are there in the original sample using the following dilution and platingseries? Make sure to label all dilutions that were done.

Lead nitrate Stock Solution— Dissolve 159.8 mg of lead nitrate in 100 mL of water to which has been added 1 mL of nitric acid, then dilute with water to 1000 mL. Standard Lead Solution— On the day of use, dilute 10.0 mL of Lead Nitrate Stock Solution with water to 100.0 mL. Standard Preparation— Into a 50-mL color-comparison tube pipet 2 mL of Standard Lead Solution, and dilute with water to 40 mL, and mix. Test Preparation— Into a 50-mL color-comparison tube dissolve 1.0g ascorbic acid (mol wt 176.12) in 25 mL of water, dilute with water to 40 mL, and mix. Procedure— To each of the two tubes containing the Standard Preparation, the Test Preparation, add 2 mL of pH 3.5 Acetate Buffer, then add 1.2 mL of thioacetamide–glycerin base TS, dilute with water to 50 mL. Mix, allow to stand for 2 minutes, and view downward over a white surface. The color of the solution from the Test Preparation is not darker than that of the solution from the Standard Preparation. The colour is formed when…

i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision

The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.

Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…

A chromatographic separation of four compounds gave the following results. The dead time (tm) is 16.7 sec. (a) What is the capacity factor for analyte 4? (b) What is the resolution factor for analytes 3 and 4?

Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed: Rf Values: (A: 0.6, B: 0.8, C: 0.2, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method? 2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the meaning of RT? *

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A vial containing 400 mg hydrocortisone sodium succinate powder for injection is to be reconstituted to produce 8 mL of injection. What is the amount of Water for Injections, BP that should be added to the powder? The displacement volume of hydrocortisone sodium succinate is 0.05 mL/100 mg.

points) You have to make 50mL of 0.05M HCI from a 10M HCI stock solution, but the onlymeasuring devices available are 100mL graduated cylinders and 10mL serological pipes. Howcould you accurately make the dilute HCI solution (clearly explain)?

Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of Acetaminophen

You need to make 20 mL of a 1/100 (10-2) dilution of a crystal violet solution? How much diluting fluid will be required? Note: Your answer should be in numerical format to the nearest 0.1 mL.

The answer is 375 cc 12% acetic acid, 625 cc water. I don't know how to get there. Please show work

Answer the ff and state references: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria  What is the total volume of the medium needed? What is the amount of each component needed, given the following media composition per liter of distilled water: Component                                 g/L tryptone 10.0 NaCl 5.0 yeast extract 10.0 agar 15.0

DILUTION COLONY COUNT CFU/mL 1:106 155000   1:107 15500   1:108 1550   1:109 155   Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?

Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.

10 mL of 0.5 M CuSO₄ * 5H₂O solution from 10 M CuSO₄ * 5H₂O solution

BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.

Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.

One hundred (100) ml of raw goat's milk bought from a street vendor is added in bottle 1 which initially contains 900 ml diluent. The sample was serially diluted by transferring 10 ml each to the succeeding bottles 2, 3 and 4 all of which contain 90ml diluent. Calculate the final dilution in bottle 4 expressed in decimal number. (Attach a screenshot of your computations at the end of the assessment). 0.01   0.001   0.0001   0.00001   0.000001

10 If3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?

Calculate the Rf of the spots from the samples. Report your answers in four decimal places.