2023 Fall BIOL2315 Manual Rate It

.docx

School

Langara College *

*We aren’t endorsed by this school

Course

2315

Subject

Biology

Date

Feb 20, 2024

Type

docx

Pages

7

Uploaded by CoachProtonSquirrel31

Report
Rate It! Submission guide Must be completed Assignment due date (yyyy-mm-dd) 2023-11-18 Last 4 digit of your student ID 3228 Group name Group- G ● Use this page as cover page for your lab report. Be sure to meet formatting requirements, and avoid plagiarism. ………………………………………………………………..Refer to important guide page 1 and 2 Please combine the following 1. Cover page: use this page as cover page. 2. Discussion. 3. Figure 1. Graph (Hand-drawn OR typed) 4. Answers to questions (typed) 5. List of references in APA style Sharing data within a group is okay. However, lab components and questions are an individual effort. No plagiarism. No copying data between different groups. No fabrication of data. The group name is assigned in the beginning of lab. Lab report is marked as 0 when students ● Copy and paste any content from the internet (with OR without reference provided). ● Copy and paste any content from other groups (plagiarism) ● Submit previous lab report ● Fabricate data ● Hand in your assignment more than 24 hr. late after the due date Page 1/7
Discussion (3 marks) will be marked for sure. Students will need scientific papers to support the discussion. • Must be typed. Do not scan your typed discussion. • Observe the general trend. Explain what your general trend (result) means. • Think carefully about where your bacterial catalase came from and support your reasoning with scientific papers. Use the temperature that you used to perform the titration when you interpret your data. This is a process called critical thinking. Be sure to discuss both types of bacteria. general trend of catalase reaction (rate of hydrogen peroxide decomposition) is decreasing meaning the amount of substrate (hydrogen peroxide) is decreasing over period of time as substate is decreasing with respect to time the number of collisions between catalase with substrate will decrease eventually which will result in decreasing rate of reaction. As the hydrogen peroxide is getting decomposed, it tells us that the catalase is working. The catalase we used came from a Staphylococcus spp. Because optimal temperature it requires to work at full efficiency is 30- 37degrees Celsius (Martin & Chaven, 1987). However, at room temperature 20-25 degree Celsius, a staphylococcus spp. (staphylococcus aureus) shows reduced activity of the catalase in converting hydrogen peroxide to water and oxygen and They work at 10-15% efficiency under room temperature (Martin & Chaven, 1987). On the other hand, catalase of the thermophile bacteria is not able to catalyze the reaction under room temperature because their optimal temperature range in high. (60- 70 degrees Celsius) (Kimoto et al., 2012). Page 2/7
Figure1: graph represents the amount of hydrogen peroxide decomposed over period. Five samples containing bacterial catalase ( Staphylococcus spp) and hydrogen peroxide of different time interval were titrated against potassium permanganate to calculate the amount of hydrogen peroxide decomposed. Orange color represent negative control and blue color represent experimental group. Lab Questions (must be typed – do not scan typed answer) Lab components and questions will be randomly selected for marking. Q1 . Define baseline in your own word for Rate it experiments (1 mark) : Baseline is a control group in this experiment because it gives us a non-enzymatic medium with everything else exactly same as the enzymatic medium. So, when we are measuring the rate of Page 3/7
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help