OreganoCH

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School

Santa Fe College *

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2000

Subject

Biology

Date

Feb 20, 2024

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docx

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Uploaded by DukeStarBaboon16

Notes Remember "Oregano Oil" means "Oregano Essential Oil" and the final report should say "Oregano Essential Oil". Materials:  Oregano Essential Oil  10 Mueller Hinton Plates  20 wafers hole punched from Qualitative Filter Paper and impregnated with Oregano Essential Oil  Escherichia coli stock  Staphylococcus aureus stock  10 cotton swabs  Bunsen Burner with gas  Tweezers  2 beakers  Alcohol  1-piece Qualitative Filter Paper  Hole punch Method: 1. Label 10 Mueller Hinton Plates and draw dividing line halving each plate 2. Punch 20 wafers from Qualitative Filter Paper 3. Drop 20 wafers into 1 beaker and drop Oregano Oil onto wafers until soaked 4. Light Bunsen Burner 5. Collect Stock of E. coli and S. aureus 6. Lawn 5 plates with Escherichia coli (using new cotton swab each plate) 7. Lawn 5 plates with Staphylococcus aureus (using new cotton swab each plate) 8. Considering the dividing line, place one Oregano Oil impregnated wafer into each half of each inoculated plate. (flame tweezers between each wafer placement) 9. Incubate all 10 plates at 37 C for 24 hours. 10. Observe after incubation period. Abstract Introduction A. Background information 1. Introduction to Kirby-Bauer Technique a. Briefly explain Kirby-Bauer technique b. Mention disk diffusion method to determine sensitivity or resistance of bacteria to specific anti microbials 2. Rationale for Choosing Oregano Oil a. Why use oregano oil as antimicrobial agent

b. Discuss known antibacterial, antifungal, antiviral effects c. Highlight previous studies that support oregano oil as antimicrobial agent 3. Background on E. coli and S. aureus a. Provide general information (characteristics, typical habitats, pathogenicity) b. Why choose these to study? 4. Antibiotic Resistance and Alternative Treatments a. Discuss global issue of antibiotic resistance and why alternative antimicrobial b. Emphasize importance of exploring alternative antimicrobial 5. Previous Research on Oregano Oil a. Summarize relevant studies b. Highlight any findings that support oregano oil as antimicrobial 6. Objectives of Your Study a. Clearly state objectives of the study (assessing the effectiveness of oregano oil against .... using .... technique) B. Thesis statement 1. Antimicrobial Efficacy 2. Target Organisms 3. Methodology 4. Broader Context Methodology A. Research design 1. Participants  Name  Name  Name 2. Materials  Oregano Oil  10 Mueller Hinton Plates  20 wafers hole punched from Qualitative Filter Paper and impregnated with Oregano Essential Oil  Escherichia coli stock  Staphylococcus aureus stock  10 cotton swabs  Bunsen Burner with gas  Tweezers  2 beakers  Alcohol  1-piece Qualitative Filter Paper

 Hole punch 3. Procedure 1. Label 10 Mueller Hinton Plates and draw dividing line halving each plate 2. Punch 20 wafers from Qualitative Filter Paper 3. Drop 20 wafers into 1 beaker and drop Oregano Oil onto wafers until soaked 4. Light Bunsen Burner 5. Collect Stock of E. coli and S. aureus 6. Lawn 5 plates with Escherichia coli (using new cotton swab each plate) 7. Lawn 5 plates with Staphylococcus aureus (using new cotton swab each plate) 8. Considering the dividing line, place one Oregano Oil impregnated wafer into each half of each inoculated plate. (flame tweezers between each wafer placement) 9. Incubate all 10 plates at 37 C for 24 hours. 10. Observe after incubation period. B. Data analysis plan  Photograph and compare  Measure and average  Compare our data with antibiotic research data on same bacteria Observation and Data A. Presentation of findings E. coli Plate Disk 1 IZ Disk 2 IZ 1 11mm 13mm 2 13mm 15mm 3 14mm 13mm 4 5mm 5mm 5 14mm 16mm S. Aureus Plate Disk 1 IZ Disk 2 IZ 1 20mm 21mm 2 20mm 25mm 3 23mm 23mm 4 20mm 22mm 5 27mm 28mm

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