MCB2010CLab5Report
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Tallahassee Community College *
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2010
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Biology
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Feb 20, 2024
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Name(s)
: Nicole Linebaugh
Section: 2360 Lec/Lab
LAB 5 REPORT
:
Preparing a Standard Plate Count of Viable Bacteria
1. Record the data from your plate observation to the table below:
Dilution Factor
Number of colonies/plate
10
6
TFTC
10
7
TFTC
10
8
TFTC
10
9
TFTC
2. Calculate the selected plate for counting (CFU/ml). 0/10^-6= 0*10^6=0 CFU/ml.
3. Which plate did you select to calculate the CFU/ml? Why this plate was selected? I had selected the 10^6 plate, but any of the other plates would have been the same since all my plates had too few to count colonies. Generally, I would select a plate if I had a number of colonies/plates between 30 and 300 and use the dilution factor that is closer to 300 for more accuracy with +/- 10% error. 4. If you were to do a total count on this sample (as opposed to a viable count), would the total count most likely to be higher or lower than the viable count? Why? The total count most likely would be higher than the viable count because the total count would consider all the cells on the plate not just the living cells as it would in the viable count.
5. Why is it often necessary to dilute a sample before spreading it on a plate for a plate count? It is often necessary to dilute a sample before spreading it on a plate for a plate count because you need to count the colonies individually; when the plate is well concentrated, it is difficult to count and have congruent growth.
6. What is the main reason to prepare a serial dilution when performing a standard/viable plate
count? The main reason to prepare a serial dilution when performing a standard/viable plate count is that the amount of concentration of the cells in the plate is to produce discrete colonies that can be countable for individual microbial growth in the 30-300 Colony range and identify the bacterium. 7. Why is it important to spread the culture liquid around on the surface of the agar when inoculating plates for a viable count? If the culture liquid is not spread, what will you see from the plate after incubation? This is important because spreading the culture liquid around on the
surface of the agar will give the sample a larger area for cells to grow and lead to a better and more accurate count. If the culture liquid is not spread, you will see clumps.
8. Attach two labeled digital images of your plates which have most and least numbers of colonies.
S. marcescens 10^-7 (Figure 1)
S. marcescens 10^-9 (Figure 2)
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Related Questions
Helping tags: Biology, bacterial count, dilution, serial dilution
WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls
show complete solutions also for the computation part. Thank you.
1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to
determine the population of the culture, by transferring 25 ml of the suspension to 225
ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9
ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The
following table shows the results of the plating method.
Sampling
COUNTS
2nd dilution
3rd dilution
4th dilution
0,0
30: 35
250;245
1st dilution
1st
2nd
3rd
0; 0
240; 235
TNTC
55; 60
5; 6
TNTC
TNCT
TNTC
TNTC
a) Illustrate the dilution series used and label the final dilution of each dilution.
b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours.
Show all computations.
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O 2.5X10^11 pfu/mL
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O 2.5X10^9 pfu/mL
O 2.5X10^8 pfu/mL
O 2.5X10^10 pfu/mL
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Cite your reference
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The throat culture obtained exhibited gram positive cocci in chains. It also showed beta-
hemolysis on sheep blood agar (SBA) and was catalase negative.
1. What was the most likely organism?
A. Streptococcus pyogenes
B. Streptococcus viridans
C. Staphylococcus aureus
D. Streptococcus pneumonia
2. What is the BEST diagnosis at the time of death?
3. What are the other possible sequela of this infection?
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using a cpt coding book
Automated blood count (CBC) with differential: 85025 (automated) or 85027 (manual)
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5. Hearing test using pure tone audiometry (air only): 92552
6. Suction lipectomy (lower right leg): 15876 (abdomen, hips, thighs, etc.)
7. Diagnostic lumbar spinal puncture: 62270 (lumbar puncture, diagnostic)
8. Direct ligation of esophageal varices: 43228 (endoscopic sclotherapy) or 43324 (open)
9. Simple pulmonary stress test: 94620 (exercise test, cardiac, maximal)
10. Intradermal Tuberculosis (TB) test: 86580 (tuberculin skin test)
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A. A
Aa A
三,三,行,E
AaBbCcDdE Aa
AaBbCcDdF
AaBbCcDdF
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A
Normal
No Spacing
Heading 1
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=y updates, fixes, and improvements, choose Check for Updates.
1. You have found a putative virus which is able to infect a bacterium causing increased
mortality and resistance to all antibacterial agents. You have systematically purified the
sample but have an unknown concentration of viruses. You perform the following serial
dilutions.
0.5ml
1ml
5ml
0.1ml
2.5ml
1ml
15ml
1
3
6
7
Phage Buffer
100.5ml
9ml
14.5ml
99.9ml 25ml
5ml 985ml
Tube Dilution in each Test Tube (Show Tube Dilution in each Test Tube (Show your
your work)
#
#
work)
4
1
6.
O Focus
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W
30
DII
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000
000
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Related Questions
- Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.arrow_forwardWil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mLarrow_forwardIn not more than 7 sentences, discuss briefly the techniques used in microscopic examination of stool. Cite your referencearrow_forward
- Please help urgentlyarrow_forwardPlease answer 4,5 and 6 Thanksarrow_forwardhttps://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleasearrow_forward
- The order is for Streptomycin 1gm IM. You have a 5gm vial of Streptomycin . The label states to add 9ml of sterile water to yield 400mq / m * l How many ml will you give?arrow_forwardCase #2 Clinical History: This 29-year-old male's illness began 10 weeks prior to death, with an episode of "flu". Two weeks later his urine became "smoky". He was found to have hematuria, albuminuria and elevated BUN (180 mg/dl). He died from a pulmonary embolus. The throat culture obtained exhibited gram positive cocci in chains. It also showed beta- hemolysis on sheep blood agar (SBA) and was catalase negative. 1. What was the most likely organism? A. Streptococcus pyogenes B. Streptococcus viridans C. Staphylococcus aureus D. Streptococcus pneumonia 2. What is the BEST diagnosis at the time of death? 3. What are the other possible sequela of this infection?arrow_forwardusing a cpt coding book Automated blood count (CBC) with differential: 85025 (automated) or 85027 (manual) 4. Urine dipstick test for ketones: 81003 (urinalysis, auto, without micro) 5. Hearing test using pure tone audiometry (air only): 92552 6. Suction lipectomy (lower right leg): 15876 (abdomen, hips, thighs, etc.) 7. Diagnostic lumbar spinal puncture: 62270 (lumbar puncture, diagnostic) 8. Direct ligation of esophageal varices: 43228 (endoscopic sclotherapy) or 43324 (open) 9. Simple pulmonary stress test: 94620 (exercise test, cardiac, maximal) 10. Intradermal Tuberculosis (TB) test: 86580 (tuberculin skin test)arrow_forward
- Answer and explain the 2 questions below. No copy-paste in google, please. What components of whole blood (can you think of), can be separated from one another by centrifugation? After collecting some water from a flooded area after heavy rain, what sediments (name at least three) do you think would you be able to collect after sedimentation and decantation?arrow_forwardA patient was prescribed 90 oz of D5W to run at 30 gtt/min. How long will it take to infuse the IV fluid? Calibration : 15 gtt/mLarrow_forwardAnswer 6-7 sentences each. pleaseee badly need it ASAP State the importance of familiarizing with the different equipment and apparatuses used in microbiology laboratory. Enumerate 5 precautionary measures to be observed to ensure safety when using various instruments used in the laboratory.arrow_forward
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SEE MORE QUESTIONS
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