BIO 120L Module 8 Simulating Methods to Estimate Population Size Lab
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Name: Nicole Rosandich
Date: December 15, 2023
BIO 120L Module Eight Lab Report: Simulating Methods
Data
Distanc
e
Transe
ct 1
Trial 1
Transe
ct 1 Trial 2
Transe
ct 2 Trial 1
Transe
ct 2
Trial 2
Transe
ct 3 Trial 1
Transe
ct 3 Trial 2
Transe
ct 4 Trial 1
Transe
ct 4 Trial 2
Transe
ct 5 Trial 1
Transe
ct 5 Trial 2
0 mm
Blue
Blue
Blue
Blue
Green
Green
Blue
Blue
Blue
Green
5 mm
Blue
Red
Blue
Blue
Green
Red
Blue
Blue
Green
Red
10 mm
Blue
Green
Blue
Red
Red
Green
Blue
Blue
Red
Blue
15 mm
Red
Blue
Red
Green
Blue
Blue
Red
Blue
Blue
Blue
20 mm
Blue
Blue
Green
Blue
Blue
Red
Blue
Red
Blue
Blue
25 mm
Blue
Green
Blue
Blue
Green
Blue
Blue
Blue
Green
Red
30 mm
Blue
Blue
Blue
Green
Green
Red
Red
Blue
Green
Blue
35 mm
Blue
Blue
Green
Blue
Red
Blue
Green
Blue
Red
Blue
40 mm
Blue
Red
Blue
Blue
Green
Blue
Red
Blue
Green
Blue
45 mm
Blue
Blue
Blue
Red
Blue
Blue
Blue
Blue
Blue
Blue
50 mm
Blue
Blue
Red
Blue
Blue
Green
Blue
Blue
Blue
Blue
55 mm
Green
Red
Blue
Blue
Green
Green
Green
Blue
Green
Blue
60 mm
Blue
Blue
Blue
Red
Green
Blue
Green
Green
Green
Blue
65 mm
Red
Blue
Red
Blue
Blue
Red
Red
Red
Blue
Blue
70 mm
Blue
Blue
Blue
Blue
Red
Green
Green
Blue
Blue
Red
75 mm
Blue
Blue
Blue
Blue
Green
Blue
Blue
Green
Blue
Blue
80 mm
Red
Blue
Blue
Blue
Blue
Blue
Blue
Red
Red
Blue
85 mm
Blue
Green
Blue
Blue
Blue
Red
Green
Blue
Blue
Blue
90 mm
Blue
Blue
Blue
Green
Green
Blue
Green
Red
Blue
Red
95 mm
Red
Red
Green
Blue
Red
Blue
Red
Green
Blue
Blue
100 mm
Blue
Red
Blue
Blue
Blue
Blue
Green
Red
Red
Green
1
Data Table 2
Color
Trial 1
Total
Trial 1
Percent
Coverage
Trial 2
Total
Trial 2
Percent
Coverage
Blue
59
56%
67
64%
Red
20
19%
22
21%
Green
26
25%
16
15%
Total Number of Squares Counted
105
100%
105
100%
1.
In terms of percent coverage, rank the colors on the Population Sheet from highest to lowest.
In both sets, blue was my highest percentage, however, the green and red switched between my first and second set. They were very close in percentage but in the first set, it was blue, green then red, and the second set was blue, red, green. 2.
How would the data change if 10 transects were run during this activity?
I would presume to see this happen repeatedly. Blue in the lead, then the red and green switching closely as second and third. 3.
In addition to percent cover, what other information could be determined about each population using the transect data that was collected?
You are able to see trends and densities with transect data. 4.
List three situations or types of organisms for which this type of population study is appropriate.
As reported in an article from the national park service, the transect is important to use for field observations to record data over time in the same area, also the can use drop pins to tally and id species of vegetation
to access vegetation coverage. The continue that, “
Without them, it would
be very difficult to represent the complexity of a natural environment.” (NPS)
I would then conclude that we would use this for vegetation, change
in habitat or small organisms with in the area. © 2016 Carolina Biological Supply Company
2
5.
Give an example of a situation where this type of population study would not be ideal.
You would not want to use this with something like adult birds, because they would be moving around way to fast to get any type of accurate count or measurements. Activity 2
Data Table 3
Quadrat #
# of Red/6.25 cm
2
# of Green/6.25 cm
2
1
61
37
2
53
39
3
62
31
4
58
37
5
63
32
Average Density/ 6.25
cm
2
59
35
Density/1 cm
2
9.504
5.632
6.
Given the density calculated for 1 cm
2
, what would the estimated population be for an area that is 20 cm by 40 cm in size?
7.
How would the data change if 10 quadrats were sampled for this activity?
More accurately determine the area.
8.
In addition to population density, what other information could be determined about each population using the quadrat data that were collected?
If the population is physically countable and you need a precise estimate. I would this this is like the census. It would give us a quadrant, like city or county or state borders. Then we can gain an accurate picture of the demographic of that region. Weather it’s household size, population density to a region, or age, or gender, or household income. © 2016 Carolina Biological Supply Company
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Activity 8
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Practice Problem 1:
The coding sequences of Gene F and Gene G are shown by the double-stranded DNA below:
Gene F
5' ATGGGAGCACCAGG ACAAGATGGATATCATTAG 3
AGTTA C CCTCGTGGT CCTGTTCTACCTATAGTA 5'
3'
Gene G
Questions:
1. Write down the messenger RNA sequence when Gene F is transcribed.
2. Write down the polypeptide chain when Gene F is completely expressed.
3. Write down the messenger RNA sequence when Gene G is transcribed.
4. Write down the polypeptide chain when Gene G is completely expressed.
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8/30
Next
Pick the correct SAR modification:
IV. Chain elongation
by extra one carbon
will increase the
activity
L Replacement of
COOH by tetrazole
ring decreases the
activity.
OH
II. Isosteric
replacement of N
with CH, will
decrease the
actrvity
IIL Removal of
Cl will decrease
the activity
O Type here to search
3r4
617
S
DEF
GYH1
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This is homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it!
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Task #3: Design primers
1 agagtctcct cagacgccga gatgctggtc atggcgcccc gaaccgtcct cctgctgctc
61 tcggcggccc tggccctgac cgagacctgg gccggtgagt gcgggtcggg agggaaatgg
121 cctctgccgg gaggagcgag gggaccqcag gcgggggcgc atgacctcag gagccgcgcc
181 gggaggaggg tcgggcgggt ctcagcccct cctcaccccc aggctcccac tccatgtggt
241 atttctacac ctccgtgtcc cggcccqgcc gcggggagcc ccgcttcatc tcagtgggct
301 acgtggacga cacccagttc gtgaggttcg acagcgacgc cgcgagtccg agagaggagc
361 cgcgggcgcc gtggatagag caggaggggc cggagtattg ggaccggaac acacagatct
421 acaaggccca ggcacagact gaccgagaga gcctgcggaa cctgcgcttc tactacaacc
481 agagcgaggc cgttgcqtga ccccqgcccg gggcgcaggt cacgactccc catcccccac
541 gtacggcccg ggtcgccccg agtctccggg tccgagatcc gcctccctga ggccgcggga
a) First you'll need to design primers to PCR-amplify amino acids 45-56.
i)
Record the sequences of the forward and reverse primers, in the 5' to 3' direction. Each
primer must be 8 nucleotides long (note that normally primers are much longer than this).
Show intermediate steps…
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Explain Potential technical issues and limitations of PCR technology are mentioned
Correct information about tissue that can be used to test for a genetic disease and justification of tissue selection
Detailed information about the position (exact base pair number) of the new mutation relative to the sequence of the PAH gene. Numbering is based on the start of transcription of the PAH gene.
PLEASE ANSWER ALLLL PLEASEE
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1.00
O b. In the direction of 3' to 5
P Flag question
O c. From DNA strand that starts with 3'
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O e. From RNA strand that starts with 5'
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Lesson 2
Focus Questions
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2. Examine the 150 base promoter sequence below.
Kaylee Kauff
5'TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG
GTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC
ACCCACGAGG AGC ATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA3'
Write in the sequence of the complementary strand and mark the 3' and 5' ends of the
complementary strand.
43
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Practice Problem 2:
The coding sequences of Gene F' and Gene G' are shown by the double-stranded DNA shown below:
Gene F
5' ATGGGAGCACCAGGATAAGATGGATATCATTAG3'
3' AGT TACCCTCGTGGTC CTATTCTA CCTATAGTA 5'
Gene G'
Questions:
1. Write down the messenger RNA sequence when Gene F' is transcribed.
Write down the polypeptide chain when Gene F' is completely expressed.
3. Write down the messenger RNA sequence when Gene G' is transcribed.
4. Write down the polypeptide chain when Gene G' is completely expressed.
5. If Gene F' were a mutant allele of Gene F, what type of mutation would be demonstrated here?
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6. If Gene G' were a mutant allele of gene G, what type of mutation would be demonstrated here?
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TEXT ANSWER
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2. Describe two similarities between the cells of prokaryotes and eukaryotes.
3. Describe one difference between the cells of prokaryotes and eukaryotes. You may
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Write your answers using 3 to 4 complete sentences.
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