Lab Report Draft - I Intro ^0 Methods Assignment (1)- Tahj

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Georgia State University *

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Biology

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Oct 30, 2023

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Biology 2107 Lab Report Draft Intro & Methods Assignment Name: _____________Tahj Hugley________________________ 2107L Lab Report Draft Spring 2022 Learning Objectives:  Apply the scientific method  Effectively communicate your scientific findings Task: Draft the introduction and methods of your lab report covering your findings from your group’s background and experimental design on the Microbiology experiments. Complete this assignment individually. Your submissions will be put through the Turnitin software on iCollege to check for plagiarism. Criteria: Use the rubric below to help guide your writing. The introduction should supply any relevant background information needed for your paper and state your hypothesis. You should have at least 3 primary sources for your introduction in addition to your lab manual and any class material you may use. Use APA formatting for your Works Cited section. The methods and materials should not be a list and only describe important methods and only mention well-known methods (i.e., solution making). The methods section should be written in the past tense. To earn full credit, this assignment is due by the start of the following lab, submitted to the proper assignment folder on iCollege. This assignment is worth 22 points. Background: Use your Instructor’s posted PowerPoint, the Lab Report Rubric, the Lab Manual, and any primary literature you’ve selected to complete the worksheet. Use this space below to put the draft of your introduction. In this experiment, the required process was to collect data in different types of medias to quantify the amount of growth observed under the specific conditions of the experiment. The goal was to determine the types of bacteria present in colonies that were successfully cultured through gram staining procedures. The first part of the lab focused on the comparing and contrasting of the differential and selective media for cultivation of pathogens. There were 3 different steps to collect the data which were the washing hands experiment, the obtaining microbial samples from the environment, and lastly testing for microbial action. The Microbiology lab consisted of the data collection and the first step was to do the hand washing experiment. It was predicted that the number of bacteria will decrease as more effort is put into washing hands. This is said because soap usually has some type of bacteria killer in it; also, when using a towel, it was believed that some bacteria will transfer for the hand towel to the hands due to the lack of sterile conditions. Next, it was required to test for the presence and diversity of species found on object in the lab. The hypothesis for this part of the experiment was that surface 1 which was an iPhone, would have much more bacteria on it than surface 2 which was the lab tables. This was thought because it was assumed that the tables were cleaned with EtOH after every class, whilst the iPhone is never disinfected. The last part of the first half of the experiment was to test the effectiveness of antibiotics on bacterial cultures. It was predicted that the penicillin wouldn’t kill a lot of bacteria, the Tetra would kill the most bacteria, the Lysol H2O2 would kill more than its All-Purpose counterpart which won’t really kill any, bleach would kill a lot of bacteria due to its familiar nature with the world and all-purpose disinfecting, and lastly the Listerine wouldn’t kill any sense it is geared more to the bacteria in the mouth.

Draft Intro & Methods Rubric Use this space below to put the draft of your methods. The experiment began at 12:00pm and ended at 2:30pm, the experiment started with the hand washing experiment. In this experiment it was instructed to wash the hands 4 different ways to determine how much bacteria were killed in the process. First was to swab the hands before washing and place it on a corner of the LB sample plate. Next was to wash the hands without soap and swab it on another corner of the same plate. Next was to wash the hands with soap and use a towel to dry them off and swab the hands and put it on another corner of the same plate. Lastly, was to scrub the hands thoroughly with soap and dry them off with a towel and swap them and put it on the final corner of the same plate. Next, was to repeat the process with another set of hands but instead of using a LB plate you are to use a TSA plate to conduct the rest of the experiment. The next step was to swab 2 different surfaces found in the lab, for this experiment an iPhone was used for surface 1 and a lab table was used of surface 2. The plate had the addition of EMB as well as LB and TSA, the plates was sectioned into halves and was labeled surface 1 and 2. The phone was swabbed and placed into the surface 1 area of the plate and the same was done for surface 2. Lastly, it was required to put 6 different types of disinfectants on the TSA plates and section them having 3 tabs of disinfectants each with both having a spot for a blank space. After all the preparation were don’t the plates incubated for a week. The next part of the experiment required to observe what happed to all the samples. After grabbing the plates, the first set consisting of the handwashing was observed and recorded by the number of colonies seen as well and the form of which they were after the process. After recording the results into the lab notebook, the next step was to place the 2 best results of the experiment onto a observing dish under the microscope using a scooping tool made sterile with the help of the Bunsen burner. After scooping the best results from the handwashing part of the experiment, the same process was completed for the 2 surface experiments consisting of the EMB plate and the phone and table. The last part of this experiment was to observe the circumference and shape of the area around the disinfectants to see if they were effective against that type of media. All the observation were recorded and placed into the lab notebook in a table specifically for it. Use this space below to put your Literature cited. Master, J. (2022). Microbiology II Gram Stain. Retrieved March 9, 2022, from https://gastate.view.usg.edu/d2l/le/content/2484115/viewContent/48632457/View Master, J. (2022). Scientific Method. Retrieved March 9, 2022, from https://gastate.view.usg.edu/d2l/le/content/2484115/viewContent/48387708/View Master, J. (2022). Microbiology I Effectiveness of Disinfectants. Retrieved March 09, 2022, from https://gastate.view.usg.edu/d2l/le/content/2484115/viewContent/48595107/View

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