EPOC Lab

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School

University of Cincinnati, Main Campus *

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Course

3032C

Subject

Biology

Date

Dec 6, 2023

Type

docx

Pages

2

Uploaded by saadnaveed414

Physiology of Exercise Lab (HLSC 3032C Lab) EPOC Lab Activity Details: The participant for this lab will don a face mask that will be connected to a computer-based program to assess VO 2 . The participant will first complete a warm-up and stretch as needed. The participant will then sit quietly on the bike to measure VO 2 for 5 minutes. The participant will complete five sprints on the cycle ergometer, cycling at 100 Watts (60 rev/min, 1.7 kg) for one minute and then at 200 Watts (120 rev/min, 1.7 kg) for a 30-second sprint, repeated five times. The specific protocol can be seen below: 0:00-2:00 – Steady @ 50 Watts (50 rev/min, 1 kg) (warm up) 2:00-3:00 – Steady @ 100 Watts (60 rev/min, 1.7 kg) 3:00-3:30 – Sprint @ 200 Watts (120 rev/min, 1.7 kg) 3:30-4:30 – Steady @ 100 Watts (60 rev/min, 1.7 kg) 4:30-5:00 - Sprint @ 200 Watts (120 rev/min, 1.7 kg) 5:00-6:00 - Steady @ 100 Watts (60 rev/min, 1.7 kg) 6:00-6:30 - Sprint @ 200 Watts (120 rev/min, 1.7 kg) 6:30-7:30 - Steady @ 100 Watts (60 rev/min, 1.7 kg) 7:30-8:00 - Sprint @ 200 Watts (120 rev/min, 1.7 kg) 8:00-9:00 - Steady @ 100 Watts (60 rev/min, 1.7 kg) 9:00-9:30 - Sprint @ 200 Watts (120 rev/min, 1.7 kg) 9:30-10:30 - Steady @ 100 Watts (60 rev/min, 1.7 kg) 10:30-12:30 - Steady @ 50 Watts (50 rev/min, 1 kg) (cool down) We will measure VO 2 , RER, and HR every 30 seconds for the entirety of the test. After the completion of the sprint intervals, we will continue to measure VO 2, RER, and HR every 30 seconds for 5 minutes while the participant sits quietly on the a cycle ergometer. He will have 5 sprints. **This worksheet will be due one week from your lab date on Canvas** Assignment: 1. What is the purpose of today’s lab? The purpose of today's lab is to measure a subject's VO2, RER, and HR every 30 seconds for 5 minutes while the person is on the bike. This will allow us to understand EPOC as well as be able to calculate calories expended. 2. What does EPOC stand for? What does EPOC tell us? Excess post-exercise oxygen consumption and it tells us how much oxygen is expended in the body after exercising. 3. What equipment was needed for today’s lab? Computer base program, a face mask, the cycle ergometer, and the metabolic cart. 4. What were the general steps to conducting lab today? The steps included 3 different phases, the Resting, Exercise, and EPOC. The person undergoing the exercise will first begin to warm up and will have his resting VO2, HR, and RER recorded. Next, the subject will go to the exercise phase in which he will have to sprint on the bike every minute for 30 seconds. We then will record the same variables and move on to the next phase.VO2 will be taken and the HR and RER for 5 minutes after the test and then calculate the number of calories expended during each phase. 5. Approximately how many Calories were expended during the five-minute resting portion BEFORE the test? 14.5 Calories
6. Approximately how many Calories were expended during the exercise portion of the test? 82.88 Calories 7. Approximately how many Calories were expended during the five-minute resting portion AFTER the test? 18.75 Calories 8. Why were there more Calories expended during the 5 minutes after the test compared to the 5 minutes before the test? In other words, physiologically, why is the body expending excess Calories after the exercise has ended? Because of the disturbance in homeostasis due to the intensity of the exercise. 9. How would EPOC change if we completed a moderate-intensity, steady-state exercise instead of sprints? Why? The EPOC would decrease because the more intense the exercise the more oxygen deficiency and metabolic disturbances you have such as lactic acid formation. 10. What is the difference between EPOC (or oxygen debt) and oxygen deficit? Oxygen deficit is the lag in oxygen uptake at the start of the exercise whereas EPOC/oxygen debt is the excess oxygen consumption after the exercise is over.
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