BIOD171 Lab 7 Notebook

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES

bio 2 from Lab Manual 4 TH edition Leboffe and Pierce

procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.

Discuss about the reagents used for agarose gel electrophoresis (not Polyacrylamide gel electrophoresis)

a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.

General Electrophoresis Questions: 1. Why do we usually use agarose for DNA and polyacrylamide for proteins?2. Compare and contrast the sample buffers for DNA and protein electrophoresis.3. Compare and contrast the running buffers for DNA and protein electrophoresis.

topic: gel electrophoresis What is the purpose of the running buffer?

procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant

briefly explain the principle behind this technique; gel-filtration chromatography

how do i complete part c and d?

Please complete this correctly thank you

ANSWER THE FOLLOWING QUESTIONS REGARDING GEL ELECTROPHORESIS 1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running 4.outline the process use in the preparation of agarose gel of 1.5 concentration 5.outline the processes/steps used in gel electrophoresis 6.name twoexamples of the dye used in gel electrophoresis 7.how do we prepare x1(concentration) TAE BUFFER form 50x TAE buffer

Please answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated.   Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurred

2nd picture only

Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.

Topic: Yeast cells (dalmau plates) What are the principles of PDA, AcA, and MA plates in media preparation? please explain all

How is fluorescence spectroscopy beneficial for pharmacological analysis ? Please shortly answer at your own words. Answer should be to the point(specific 4-5 lines).

Sample Station 7 A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?

i. Illustrate the dilution series used and label the final dilution of each dilution.

covert the procedure to passive voice and past tense

Agarose Gel Electrophoresis Questions: 1. What determines how much agarose you should use in your gel? 2. Why do you see only DNA on the gel, and not protein? 3. How do you know which end of the gel to place the comb? 4. Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?

Thank you so much

Smear Preparation and Staining Lab Smear Preparation 1. What are two goals of a good smear? 2. Why does a bacterial smear need to completely air dry prior to heat fixing? 3. What is the purpose of heat fix step? Simple Stain 1. What is the difference between simple stain and complex stain? 2. Describe the color of an S. aureus after performing simple stain with Safranin Red. Why? 3. What do you conclude after performing simple stain and observing "Purple cells longer than they are wide Gram Stain 1. How does the bacterial cell structure contribute to the mechanism of Gram staining? 2. What is the mordant in Gram stain and why you need it? 3. Why must young cultures be used when doing a Gram stain? Negative Stain 1. а. Name the bacterium used in negative stain b. Describe morphology С. Name the disease caused by this bacterium 2. Describe what you observe under microscope in negative staining.

microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?

GROWTH CUVRVE Can someone help me with this? (This is a question from GFP experiment I did, which I have to include in my rapport and hand in)

for E-coli- Describe results for Gram reaction, cell shape and arrangement (look up information) What color changes were observed for each test? Any zones of inhibition (for antibiotics only)?

Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates.  Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each.    Description:           Isolate A – Describe:    Colony morphology:    Medium & incubation temperature: Isolate B – Describe:    Colony morphology:    Medium & incubation temperature:

My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium sulphate?) 1)Casein Isolation from MilkExperimental Procedure* Put 100 ml of milk in a 500 ml beaker and heat it to 40 0C. * Also heat 100 ml acetate buffer and slowly add it to the beaker with milk while stirring. * The final pH of the mixture should be 4.8. * Cool the suspension to room temperature and wait 5 more minutes before filtering. * Wash the precipitate several times with small volumes of water and then suspend it in approximately 30 ml of ethanol.Filter the suspension in the buchner funnel and wash the precipitate a second time with a mixture of equal proportions of ethanol and ether. * Finally, wash the precipitate on filter paper with 50 ml of ether and soak and dry. * Make the ether evaporate by spreading the powder. * Weigh the casein and calculate the percentage yield of the protein. * Compare with theoretical yield of 3.5 g per 100 ml milk. 2)Purification of Egg…

Complete

Factors which can influence antibiotic susceptibility testing (size of zone inhibition) are_________________.

Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…

For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. What are the concentrations of antibiotic applied in the test?

using sample prep aka the protocol use it to fill in the tables

Please answer the questions in the grey colored box provided in the image.

Test Description Media Indicator Incubation Results components or system design Nutrient agar Triphenol deeps with a tretrazolium concentration (TTC) can be gradient of temperature (°C) Motility Aerobes will be found growing at the surface of the tube and 37 Test Medium (MTM) sometimes along the stab line when oxygen is introduced during inoculation. added better охудen visualize the growth Obligate anaerobes will be found near the bottom of the tube. Facultative anaerobes will grow throughout the tube, but because more ATP is produced aerobically growth is heaviest at the surface. Microaerophile wil1 be found growing near the narrow region where oxygen concentration is limited. patterns. Brewer's Anaerobic Agar (BAA) Anaerobic Jar 4 Candle Jar 2. 3.