Lab Assignment 1

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University of Manitoba *

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1010

Subject

Biology

Date

Dec 6, 2023

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pdf

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4

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Lab assignment 1: Microbiology and microorganisms Answer the questions directly in the space provided. Do not attach additional pages. This is an individual assignment. By entering your name below, you certify that you have completed the lab yourself, and the answers given are your own. Copying assignments of other students, or other forms of plagiarism will result in a grade of zero, and will be reported to the Faculty of Science. Name: ____________________________________ Student number: _____________________ When your assignment is complete, submit it to the assignment 1 drop box on UM Learn. This assignment is due at the end of Lab 1. Exercise 1: Getting to know your microscope 1) When should you use the coarse focus knob on the microscope? When should you use the fine focus knob? (2 marks) 2) How should you position the condenser on the Nikon E100 brightfield microscope? What does the condenser do? (1 mark) 3) How should you position the iris diaphragm when viewing a colored specimen, like stained bacteria? Explain why. (1 marks) Exercise 2: How can you tell the difference between prokaryotes and eukaryotes 4) Examine the picture showing the mixed bacteria (3 types) slide in the online Lab 1. What three cell shapes are shown on this slide? (1 mark)
5) Explain why we almost always use the 100x oil immersion objective when viewing bacteria with the microscope in the lab (rather than using lower powered objectives, like the 40x). (1 mark) 6) Examine the picture of the Halobacterium salinarum slide. What shape are the cells of Halobacterium salinarum ? (0.5 marks) 7) How does the cell size of Archaea compare to that of the cells in the mixed bacteria (3 types) slide? (Look carefully at all of the bacterial cells, including the largest on the slide.) Can you tell the difference between Archaea and Bacteria by size? (1 mark) 8) Is Anabaena a prokaryote or a eukaryote? (0.5 marks) 9) Examine the picture of Anabaena . Are any special structures shown? If so, which one(s)? (1 mark) 10) Examine the short video of Euglena . In the cells in the video there is a red spot clearly visible. What is this red spot? (0.5 marks) 11) Examine the picture of Saccharomyces cerevisiae . How was the condenser positioned to take this picture? Explain why. (1 mark) 12) How do the cells of S. cerevisiae compare to the cells of prokaryotes that you observed on previous slides? (2 marks)
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