LabModule 4 Osmosis

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Clark College *

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Biology

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Dec 6, 2023

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docx

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Activity 4 Effects of Hypotonic and Hypertonic Solutions on Plant cells In this exercise, you will observe what happens to plant cells (elodea) when placed in solutions of varying tonicity. Hypothesis: Tonicity of a solution affects the water content and structure of plant cells. Predictions: PLANT CELL in hypertonic solution Ceus will ghaink PLANT CELL in hypotonic solution cels wilh %m,u ¥ (Uptuce Observation: Watch the video https://youtu.be/zVvHn6S 9PQ to make observations and sketch the cells. S AL NEIY Results: PLANT CELL in hypertonic solution (20% sucrose 0:00-0:23) cells Bhunk PLANT CELL in hypotonic solution (water 0:24-0:38) another good video of hypotonic is (https://youtu.be/pFsty-XyLZc)- conon) cevs %(w)
Activity 3 Effects of Hypotonic and Hypertonic Solutions on Red Blood Cells In this exercise, you will observe what happens to red blood cells (RBC) when placed in solutions of varying tonicity. Blood cells are very small cells, so examples use oil immersion lens to observe the effects of these solutions Hypothesis: Tonicity of a solution affects the water content and structure of red blood cells Predictions RBC in isotonic solution— C€1| {}Jv\\ emain ‘,‘I\C}\On’.l‘fij RBC in hypertonic solution = CE|| (11| "/‘n“flK RBC in hypotonic solution - C €| i\l Q oW Observation: Watch this video of https://www.youtube com/watch?v=A8cl6FkcG4c. Pause the video at 1 51, 242 and 3:18 to make observations and sketch the cells Results RBC in isotonic solution (lj in 0.9% NaCl @ 1500x) - CE\S cemain ¥E Some (unchanged ) Qe O ZRO o RBC in hypertonic solution (2:42 in 10% NaCl @1500x) Ceus shrink 5 (BWelLT tucst) %;’;%% RBC in hypotonic solution (3:18 in water @1500x) A AR Y LG\\D‘fuT)hAVQ
Results | Treatment - Ul)%fh’dll()’l}‘, | Potato strip in Tap )OO0 S0P Was laroec Water Potato strip in salt | . DMANEC + D04 Water | Y | Control Pma?o strE'T Table 2: weight of potato strips before and aofter treatment with different tonicity liquids. 1’ ;Etment | In’mra—lamght of Potato ! Welgfi; of Potato Strip | Difference nnVVéJght (use | | Strip (g) J after 1 hour (g) | +gor-g) Tap Water [\ 30 nor own o tod | R | e SCal€=| SaltWater | | .‘ —_— Afi el el Conclusions: Briefly explain your results. Be sure to correctly use the following terms: Hyperosmotic, Hypo-osmostic, Hypotonic, Hypertonic, Osmosis, Diffusion, Concentration gradient and selectively permeable nv\;\rfnt?/rane -“he Nmmplgfrt] Wwo +he +UP (,()[,[ft‘(‘( \\L,\‘OTOTMC Solu hOn\ ecame '()ni)fi()&r ¥ hardéned because 1+s warer Concepy, o N NCréeasey (d.wug.orifiw{« watee hasa higher Concentration grachent; which cauges i+ k)(jo Through +he Sekective pecmeable vemprane nto Jhe porato. The QONOY0 Dlocey WO +he Sai- wo»er(mp@rtomc Soluton) SUnK 9ot SoRtec bbacause ¥ Water Conentration ci“"““f S.Lnbs:sB
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