Lab Assignment_Bacterial ID with PCR(1)

topic: gel electrophoresis . What are other staining methods that can be used for PAGE?

topic: bacteria - please do not just copy from google   2. what cell parts are visible on the bacteria?

Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48   Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis?   2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?   4. What is the use of ethidium bromide and why must you wear gloves when you handle it?   5. What makes the DNA fragment move towards the positive plate?   6. What is the purpose of glycerol in the sample buffer?    7. What is the use of a DNA ladder?    8. What will happen when you increase the voltage of the set-up?   9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). Just answer objectives and  principles.

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved, methodology and redults and discussions. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). Just answer results and discussions and objectives.

Please answer fast You are performing a acid fast stain on acid fast -negative bacteria and you skip the decolorizer step. What is the appearance of the bacteria at the end of the stain? purple pink blue colorless

Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel

Discussion for lab report of isolation, purification and quantification of bacterial DNA.

Give typed full explanation

The gel below shows the results for five samples. Using the gel, determine the genotype of samples.

help?

Is Table Window Help 1 Exam_2_problem_set_new- Saved to my Mac Design Layout References Mailings Review View AaBbCcDdF AaBbCcDdF AaBbCcDd AaB Normal No Spacing Heading 1 Не s, fixes, and improvements, choose Check for Updates. 2. Calculate the effective dilution. You were given a sample which contained 5.5mL of bacterial culture. You added 10uL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?

Can your draw it out and explain it to me please

Create a dichotomous key please

Discuss about Fluorescencemicroscopy

+ courses/_518299_1/cl/outline?customClassicLocation=%2Fwebapps%2FBb-McGraw Hill-BB5744b9beb8ccb%2Fapp%2Flink%2Finbou.... File Edit Format Tools Help Qa 11 Chapter 2 ● Exam 2 Review Sheet ● Normal ● ● ● Unsaved Chantor 7 Edits will not be automatically saved. Calibri T Save now 14 ▾ BIU A A- EEEE VE What are the important characteristics of atoms and elements? What are the different types of chemical bonds? What are the various types of chemical reactions and solutions? What determines acidity and alkalinity, and how do they relate to the pH scale? What makes carbon the fundamental element of life? What are the four types of biological macromolecules, and what are their general structures and functions?

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes objectives, methodology, and results and discussions. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content).   ANSWER OBJECTIVES, METHODOLOGY, RESULTS AND DISCUSSIONS. cite references if possible thanks

Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). another: What is the advantage of the Gram stain over the simple stain? What is the theory about the mechanism of the Gram-stain reaction?

label image from options.

3d bioprinting systems, biomaterials, tissue engineering.Find a creative project topic on the above-mentioned area.

Instructions: Species name 2: Sample illustration: Taxonomic account (domain to species): At least two phenotypic methods used in its identification (Describe concisely). At least two genotypic methods used in its identification (Describe concisely). References: MICROBE TAXONOMIC ACCOUNT Choose a microorganism from among the microbial groups. Fill in the information required for your chosen microbe.

Hello, good day. I am having a problem answering this question and I need your help. Hoping for a response and thank you so much. Question: What are the general rules in grams staining classification of bacteria?

les/test/tq.php?testid=2804&strandid%3D&element%3D&difficulty=assessment&assignment_id%3D45196931&load_test%-D1&teacherPr B Brainly.com-For st.. O Instagram N Netflix a Escape Room (Und. USATestprep, LLC - E Google Docs Stu wrerhd.cells at rer rote due to the siiple diffsion of Save C) 01ann suan an sianua LUPInaPLiaipaih ep snan aun aAPa IM JaPAA active transport. Glucose molecules will move from the cells into the solution in the beaker D) through facilitated diffusion. When examining the major macromolecules in the cell, a student isolates two molecule types that are directly responsible for cellular energy. A comparison of which molecule types will be most likely to include the molecules primarily responsible for cellular energy? A) proteins and lipids B) carbohydrates and lipids proteins and carbohydrates D) carbohydrates and nucleic acids Proteins and polysaccharides are polymers. These polymers are formed by dehydration synthesis. Which statement correctly identifies a…

Can you help me, please?

microbial sensitivity lab: Why should you avoid looking directly into the ultraviolet (UV) light?

Hello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given) *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.

Please answer question as best as possible. The identified organism is E. coli . Thank you

INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: Discuss the various theories on the Gram stain.

Make a dichotomous key for beauty product or cleaning agent.at least 10 items. plse this is the second time i am sending this assignment. someoen send me the answer but all the images are blurry. plse make sure the image is clear. thank

Disrobe  laboratory method Western Blot  used to evaluate the immune response, describe how it works in detail, Also, please provide at least one pathogen your method of analysis is used to detect.