Mid-term_1_2021
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Johns Hopkins University *
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Dec 6, 2023
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Tissue Engineering 580.442 / 580.642
MID-TERM EXAM – 1 (Fall 2021)
This is a CLOSED BOOK exam. No notes, lecture slides, or other class materials should be
available to you electronically or otherwise. You should be logged into the regular class
zoom link with your video on while doing the exam. Question 1 – 9 points
Question 4 – 13 points
Question 2 – 12 points
Question 5 – 11 points
Question 3 – 15 points Total = 60 points
1.
A flow chamber such as the one shown below may be used to quantify the strength of
adhesion of cells to the underlying substrate. Cells are attached to the bottom surfaces
and a fluid flows with a laminar profile through the chamber.
a.
Describe how this system might be used to measure the strength of cell adhesion
to the substrate. (
Do NOT
include any equations for this explanation – just
describe the concepts with the key assumptions that might allow an experimenter
to use flow to measure adhesion strength ). (
4 points
)
Since the flow applies pressure onto adherent cells, but not adherent cells, the strength of
adhesion can be determined by the detachment tension for adherent cells from the surface of
substrate when the fluid flows through the chamber. So if the fluid flow rate is known, we can7
calculate the strength of cell adhesion to the substrate.
b.
Draw and label the main components of a focal adhesion to illustrate the
extracellular matrix substrate, the binding proteins, at least 3 adaptor proteins, and
the cytoskeletal proteins, connections to the nucleus. (
5 points
).
2.
Fibroblasts have been embedded in a 3D hydrogel substrate with binding sites as shown
below. The hydrogel is tethered to posts at the top and bottom of the images but the long
edges are not tethered. Over time, the hydrogel morphology changes as shown in the
figure. a.
Based on our discussions on how cells interact with their environments, sketch
and describe what you expect the morphology and orientation of a random cell
located in the center of the hydrogel
to look like at each time point (i.e. Your
answer should include 4 sketches of cells at Days 0, 7, 14, & 21 highlighting any
changes in morphology that occur over time). Explain the reasoning behind your
response. (
8 points
).
b.
Copy your sketch for the cell alignment at Day 21 only. In this sketch, represent
the alignment of the stress fibers
in the cell. On 2 or 3 of these stress fibers, add
arrows indicating what
direction the stress (tension) in the fiber is acting.
(
2 points
)
c.
Sketch for Days 0 and 21 how would your might change if there were no binding
sites in the gel to which the cells could attach (
2 points
)?
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einternational.academy/mod/lti/view.php?id=9599
O
CE / Course Access
The human body has
True
False
Back Next
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I
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stion Completion Status:
QUESTION 4
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O A. Traffic flow
O B. Air humidity
O C. compressed air
D. in-line gas and suction outlets
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