Microbiology Lab problems

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School

Carleton University *

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2303A

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Biology

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Dec 6, 2023

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pdf

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13

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11/28/23, 11:01 PM Microbiology Lab Final Exam | Quizlet https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true 1/13 Name: Score: 35 True/False questions Definition Term MSA shows what? If the organism is a lactose fermenter True False 1 of 70 Definition Term Define caliform bacteria rod-shaped, Gram-negative, non-spore forming, and motile or non-motile bacteria which can ferment lactose with the production of acid and gas True False 2 of 70 Definition Term What are the possible results of XLD test? yellow indicates acid production red with some black growth indicates sulfur reduction True False 3 of 70 Definition Term What could be the results of an O/F glucose test? 2 tubes, one with oil (anaerobic) and one not: yellow in anaerobic means fermenting glucose but if yellow only in aerobic, then oxidizing glucose True False 4 of 70
11/28/23, 11:02 PM Microbiology Lab Final Exam | Quizlet https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true 2/13 Definition Term Bile esculin shows what? can the organism use citrate as its sole source of carbon True False 5 of 70 Definition Term Describe gamma growth on blood agar ferric citrate True False 6 of 70 Definition Term Describe beta growth on blood agar nothing, still red True False 7 of 70 Definition Term O/F glucose tests show what? does the organism oxidize and/or ferment glucose True False 8 of 70 Definition Term MSA stands for triple sugar iron True False 9 of 70
11/28/23, 11:02 PM Microbiology Lab Final Exam | Quizlet https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true 3/13 Definition Term Name the selective medias used in class Blood Agar Bile Esculin Mannitol Salt Agar (MSA) MacConkey Agar (MaC) Eosin Methylene Blue (EMB) Hektoen Enteric Agar (HEA) Xylose Lysine Desoxiolate (XLD) True False 10 of 70 Definition Term Define catalase enzyme an enzyme produced chiefly by certain bacteria, having the property of cleaving DNA molecules at or near a specific sequence of bases (restriction sites) True False 11 of 70 Definition Term What is the indicator in MSA whether the fermentation takes place or not? phenol red True False 12 of 70 Definition Term HEA is used for? isolates salmonella & shigella bacteria True False 13 of 70
11/28/23, 11:02 PM Microbiology Lab Final Exam | Quizlet https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true 4/13 Definition Term What two types of organisms is bile esculin good at differentiating? enterococci and streptococci True False 14 of 70 Definition Term What machine is used to carry out a PCR reaction? phenol red True False 15 of 70 Definition Term XLD stands for xylose lysine desoxiolate True False 16 of 70 Definition Term Catalase tests for what? does the organism have catalase True False 17 of 70 Definition Term Starch tests for what? can the organism break down starch True False 18 of 70
11/28/23, 11:02 PM Microbiology Lab Final Exam | Quizlet https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true 5/13 Definition Term What are the possible outcomes of a MaC test? good growth indicates a gram negative bacterium that is not inhibited by crystal violet or bile. pink to red growth without bile indicates that the organism produces acid from lactose fermentation and is a probable coliform. colorless growth is a non lactose fermenting organism and noncoliform. True False 19 of 70 Definition Term What could be the results of a bile esculin test? black is + True False 20 of 70 Definition Term What are the possible results of a HEA test? pink growth indicates lactose fermenter and sulfur reducer True False 21 of 70 Definition Term Name the differential medias used in class pink growth indicates lactose fermenter and sulfur reducer True False 22 of 70
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