Microbiology Lab problems
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Carleton University *
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2303A
Subject
Biology
Date
Dec 6, 2023
Type
Pages
13
Uploaded by SuperHumanClover11990
11/28/23, 11:01 PM
Microbiology Lab Final Exam | Quizlet
https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true
1/13
Name:
Score:
35 True/False questions
Definition
Term
MSA shows what?
If the organism is a lactose fermenter
True
False
1 of 70
Definition
Term
Define caliform bacteria
rod-shaped, Gram-negative, non-spore
forming, and motile or non-motile bacteria
which can ferment lactose with the
production of acid and gas
True
False
2 of 70
Definition
Term
What are the possible results of XLD test?
yellow indicates acid production
red with some black growth indicates sulfur
reduction
True
False
3 of 70
Definition
Term
What could be the results of an O/F glucose
test?
2 tubes, one with oil (anaerobic) and one
not:
yellow in anaerobic means fermenting
glucose but if yellow only in aerobic, then
oxidizing glucose
True
False
4 of 70
11/28/23, 11:02 PM
Microbiology Lab Final Exam | Quizlet
https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true
2/13
Definition
Term
Bile esculin shows what?
can the organism use citrate as its sole
source of carbon
True
False
5 of 70
Definition
Term
Describe gamma growth on blood agar
ferric citrate
True
False
6 of 70
Definition
Term
Describe beta growth on blood agar
nothing, still red
True
False
7 of 70
Definition
Term
O/F glucose tests show what?
does the organism oxidize and/or ferment
glucose
True
False
8 of 70
Definition
Term
MSA stands for
triple sugar iron
True
False
9 of 70
11/28/23, 11:02 PM
Microbiology Lab Final Exam | Quizlet
https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true
3/13
Definition
Term
Name the selective medias used in class
Blood Agar
Bile Esculin
Mannitol Salt Agar (MSA)
MacConkey Agar (MaC)
Eosin Methylene Blue (EMB)
Hektoen Enteric Agar (HEA)
Xylose Lysine Desoxiolate (XLD)
True
False
10 of 70
Definition
Term
Define catalase enzyme
an enzyme produced chiefly by certain
bacteria, having the property of cleaving
DNA molecules at or near a specific
sequence of bases (restriction sites)
True
False
11 of 70
Definition
Term
What is the indicator in MSA whether the
fermentation takes place or not?
phenol red
True
False
12 of 70
Definition
Term
HEA is used for?
isolates salmonella & shigella bacteria
True
False
13 of 70
11/28/23, 11:02 PM
Microbiology Lab Final Exam | Quizlet
https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true
4/13
Definition
Term
What two types of organisms is bile esculin
good at differentiating?
enterococci and streptococci
True
False
14 of 70
Definition
Term
What machine is used to carry out a PCR
reaction?
phenol red
True
False
15 of 70
Definition
Term
XLD stands for
xylose lysine desoxiolate
True
False
16 of 70
Definition
Term
Catalase tests for what?
does the organism have catalase
True
False
17 of 70
Definition
Term
Starch tests for what?
can the organism break down starch
True
False
18 of 70
11/28/23, 11:02 PM
Microbiology Lab Final Exam | Quizlet
https://quizlet.com/86886730/test?answerTermSides=2&promptTermSides=6&questionCount=70&questionTypes=10&showImages=true
5/13
Definition
Term
What are the possible outcomes of a MaC test?
good growth indicates a gram negative
bacterium that is not inhibited by crystal
violet or bile.
pink to red growth without bile indicates
that the organism produces acid from
lactose fermentation and is a probable
coliform.
colorless growth is a non lactose fermenting
organism and noncoliform.
True
False
19 of 70
Definition
Term
What could be the results of a bile esculin test?
black is +
True
False
20 of 70
Definition
Term
What are the possible results of a HEA test?
pink growth indicates lactose fermenter and
sulfur reducer
True
False
21 of 70
Definition
Term
Name the differential medias used in class
pink growth indicates lactose fermenter and
sulfur reducer
True
False
22 of 70
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