Lung Volumes and Capacities

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Faith, Nelson, and Helena Physiology Lab 141 Dr. Borsch April 11, 2017
Abstract Normal breathing involves the circulation of 1/10th of the total lung capacity. In order to evaluate other capacities, maximum amount of effort of inhalation and exhalation was carried out to measure the differences when breathing while relaxed versus breathing with a lot of effort. The breathing cycle starts by the diaphragm contracting and flattening downward, as the chest expands outward. As this happens, the volume increasing brings air in through the nose and mouth. Once the inhaled air in the lungs has reached a high volume and pressure, exhalation occurs and the chest wall, diaphragm, and lung tissue recoil. In this experiment, lung volumes were measured during normal breathing and with maximum effort of inhalation and exhalation. Once these processes were evaluated in both relaxed and maximum efforts, data points and graphs were collected and comparisons were made between participant lung capacities, differences in female and male capacity values, and between volumes of air passed with maximum and relaxed effort. Procedure Materials Used - LabQuest - LabQuest App - Vernier Spirometer - Disposable Mouthpiece - Disposable Bacterial Filter - Nose Clip (Procedure Taken from the Lung Volumes and Capacities Experiment) 1. We began by connecting the Spirometer to LabQuest and choose New from the File menu. 2. On the Meter screen, “Rate” was tapped, and we changed the data-collection rate to 100 samples/second and the data-collection length to 60 seconds. Then we selected “OK”. 3. Next, we attached the larger diameter side of a bacterial filter to the “Inlet” side of the Spirometer. Then attached a gray disposable mouthpiece to the other end of the bacterial filter.. 4. The Spirometer was held with one or both hands as our arm(s) were braced against a solid surface, and choose Zero from the Sensors menu. 5. From here, we collected the inhalation and exhalation data by first putting on the nose plug, then by taking normal breaths, we began data collection with an inhalation and continued to breathe in and out. After 4 cycles of normal inspirations and expirations we filled our lungs as deeply as possible for maximum inhalation, and then exhaled as fully as possible for maximum expiration. This was followed with at least one additional recovery breath. 6. From here, we stopped data collection. 7. We then viewed the graph based on volume vs . time, tapped the y-axis label and selected “Volume”. We had noticed some drift in the baseline on the graph, and choose “Baseline Adjustment” from the “Analyze” menu to bring the baseline volumes closer to zero, and selected “OK”. 8. From here, we determined the D y for the Tidal Volume portion of the graph. We did this by selecting a representative peak and valley in the Tidal Volume portion on the graph. We tapped the peak and
noted the volume value. Then, tapped the bottom of the valley that followed it and noted the volume value. Then, calculated the D y value and recorded it to the nearest 0.1 L as the total Tidal Volume. 9. Next, we determined the D y for the Tidal Volume portion of the graph. We tapped the peak that represented the maximum inspiration and noted the volume value. Using the level of the peaks graphed during normal breathing from Step 8, we calculated the D y value and recorded it to the nearest 0.1 L as the total Inspiratory Reserve Volume. 10. Next, we determined the D y for the Tidal Volume portion of your graph by tapping the valley that represented our maximum expiration value and noted it. From here, using the level of the valleys graphed during normal breathing again from Step 8, we calculated the D y value and record it again to the nearest 0.1 L as the total Expiratory Reserve Volume. 11. From here we could calculate the Vital Capacity and estimated it to the nearest 0.1 L (in Table 1) using the equation provided below. VC = TV + IRV + ERV 12. After this, we could calculate the Total Lung Capacity and enter the total to the nearest 0.1 L (in Table 1) with the provided equation below. (Using the value of 1.5 L for the RV.) TLC = VC + RV Results Table 1 Volume measurement (L) Helena’s (L) Nelson’s (L) Faith’s (L) Tidal Volume (TV) 1.91 1.59 1.69 Inspiratory Reserve (IRV) 0.49 0.55 0.72 Expiratory Reserve (ERV) 0.65 2 1.13 Vital Capacity (VC) 2.84 3.76 3.65 Residual Volume (RV) ≈1.5 ≈1.5 ≈1.5 Total Lung Capacity (TLC) 4.34 5.26 5.15 Data Calculations in Table 1 formated from data points in graphs below:
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Graphs of Data Points taken from Labquest:
Helena’s Try: Nelson’s Try: Faith’s Try: Data Analysis 1. What was your Tidal Volume (TV)? What would you expect your TV to be if you inhaled a foreign
object which completely obstructed your right mainstem bronchus? - Helena’s Tidal Volume was 1.91L. Nelson’s Tidal Volume was 1.59L. Faith’s Tidal Volume was 1.69L We would expect the Tidal Volume to decrease if there was a foreign object inhaled and obstructing the right mainstem bronchus as the circulation to the right lung would be cut off. 2. Describe the difference between lung volumes for males and females. What might account for this? - The differences between lung volumes for males and females is size of thoracic cavity. Size and shape of the person would account for this, females have 10-12% smaller thoracic cavities than males ( Bellemare ). 3. Calculate your Minute Volume at rest. (TV ´ breaths/minute) = Minute Volume at rest. If you are taking shallow breaths (TV = 0.20 L) to avoid severe pain from rib fractures, what respiratory rate will be required to achieve the same minute volume? - (1.91L)(14) = 27.74 - (0.20) (x) = 27.74. X = 138. You would need to take 138 breaths per minute to achieve the same minute volume. - 1.59L)(15)=23.85 - (0.2)(X)=23.85 X=119.25 (breaths per minute to achieve the same minute volume) 4. Exposure to occupational hazards such as coal dust, silica dust, and asbestos may lead to fibrosis , or scarring of lung tissue. With this condition, the lungs become stiff and have more “recoil.” What would happen to TLC and VC under these conditions? - Scarring of the lung tissue would cause a decrease in lung function due to the scar tissue causing a thickening of the lung walls. This can also cause a decrease in the amount of oxygen supply in the blood stream and would leave patients with a decreased Total Lung capacity and Vital capacity (Wikipedia). 5. In severe emphysema there is destruction of lung tissue and reduced recoil. What would you expect to happen to TLC and VC? - In emphysema there is a loss of function and damage to the alveolar walls in the lungs and a reduced recoil. With emphysema, the lung empties slower which means that the lungs would chronically overinflate. This would lead to an overall high total lung capacity and leads to an inefficiency in lung function. Because the lungs are emptying slower and less efficiently, this would lead to a lower vital capacity (Johns Hopkins). 6. What would you expect to happen to your Expiratory Reserve Volume when you are treading water in a lake? - When treading water in a lake, there is an increase of pressure on the body from the surrounding water. This additional water pressure on the body would cause an increase in the Expiratory Reserve Volume. Discussion The results shown in the graphs relating to “lung-volume capacity” and “flow rate” portray a very
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similar pattern. However, the results show that Nelson carries the greatest Total Lung and Vital Capacities while Helena carries the least Total Lung and Vital Capacities. This may add to the statement mentioned in question #2 (above), accounting the differences between lung volumes for males and females. Such as the size of the person, the shape of the person, and the statistical analysis of females having 10-12% smaller thoracic cavities than males. This can also explain why Nelson carries the greatest expiratory volume, because with the extra lung capacity, he can exhale more air. However Nelson also carries the lowest Tidal Volume; how this happens may be from the extra air space in the lungs, a weaker diaphragm, or less oxygen required. Helena is shown to have the greatest Tidal Volume, which may be because of the least Total Lung and Vital Capacities, which may also require a higher rate of breathing in and out. Additionally, Faith is shown to have a higher rate of air inhaled (Inspiratory Volume), possibly because of a stronger diaphragm or perhaps the lung capacities were underestimated. Conclusion As we saw in this experiment, the lungs have a large volume capacity and inhalation/exhalation values can vary greatly depending on the amount of effort exerted. Individuals have various lung capacities, and while differences were seen, not a huge amount of difference was seen in the capacities of male lungs and female lungs. This could be explained by the fact that even though female lungs are smaller, they accommodate for their size due to mechanical advantages from a greater contribution of the rib cage muscles. Overall, we see that the more effort put into inhalation/exhalation, the more lung capacity that is used and that the lungs are able to hold basically double the amount of air compared to the normal amount of air that is taken in during normal, relaxed respirations References Bellemare F . (2003). NCBI. Sex differences in thoracic dimensions and configuration. Retrieved from: https://www.ncbi.nlm.nih.gov/pubmed/12773331
Johns Hopkins. (1995). Interactive respiratory physiology. Retrieved from: http://oac.med.jhmi.edu/res_phys/Encyclopedia/Emphysema/ Emphysema.HTML Wikipedia (2017). Pulmonary fibrosis Retrieved from: https://en.wikipedia.org/wiki/Pulmonary_fibrosis