BIO 150 Lab 6 Report
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Bio 150 – Laboratory #6 Report
Human Anatomy & Physiology I
DCCC
Lab Report # 6 - Microscopy, Cells, and Mitosis
Name: Kyrsten Paoli
I. The Microscope
1.
Which objective provides the largest field of view?
4X
2.
What is the function of the stage?
Holds/supports the specimen.
Brings specimen to focus.
3.
What is the magnification of the ocular lens?
10X
4.
Which objective lens should be put in place prior to placing
the microscope back in its storage compartment?
4X
5.
Why is focusing done with the fine focus knob only when using the 10X, 40X, or
100X objectives?
You can run the slide into the objective lens damaging or cracking the slide
6.
Give the total magnification obtained when using the
low power (10X) objective?
100X
II. Cell Cycle & Mitosis
B. Prepared slide of mitosis in Whitefish blastula
Interphase
1.
Find a cell in interphase and draw
a cell in interphase viewed using the 40x
objective.
2.
Label the following structures:
Cell membrane
Nucleus
Chromatin
1
8
Last modified: 01/2023 WJ
Page
Bio 150 – Laboratory #6 Report
Human Anatomy & Physiology I
DCCC
Stage 1: Prophase
1.
Find a cell in interphase and draw
a cell in prophase viewed using the 40x
objective.
2.
Label the following structures:
Cell membrane
Sister chromatids
Cytoplasm
Stage 2: Metaphase
1.
Find a cell in interphase and draw
a cell in metaphase viewed using the 40x
objective.
2.
Label the following structures:
Sister chromatids
Metaphase plate
Cytoplasm
Cell membrane
Stage 3:
Anaphase
1.
Find a cell in interphase and draw
a cell in anaphase viewed using the 40x
objective.
2.
Label the following structures:
Chromosomes
Spindle fibers
Cytoplasm
Cell membrane
2
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Last modified: 01/2023 WJ
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Bio 150 – Laboratory #6 Report
Human Anatomy & Physiology I
DCCC
Stage 4:
Telophase
1. Find a cell in interphase and draw
a cell in telophase viewed using the 40x
objective
.
2. Label the following structures:
Chromosomes / chromatin
Cleavage furrow (cytokinesis)
Cytoplasm
Cell membrane
III. Observation of Buccal Cell using a Wet Mount
1.
Draw one buccal cell observed using the 40x objective.
2.
Label the following structures:
Nucleus
Nuclear envelope
Cytoplasm
Cell membrane
Instructor observation
Instructor Microscope Check
Next page..
3
8
Last modified: 01/2023 WJ
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Related Questions
Please see attached . Thank you
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Anatomy and Physiology I
Chapter Three The Microscope
Exercise 3.5: Determining Depth of Field
6. Referring back to exercise 3.5, record the answers to the questions about colored threads in the spaces provided. 7
Color of bottom thread:
Color of top thread:
Color of middle thread:
* Explalm why proper microscope technique requires always viewing a slide with the scanning objective first before moving to higher-power objectives. Use the concept
of depth of field in the explanation. 7
Exercise 3.6: Parts of a Dissecting Microscope
8. Identify two differences between a dissecting microscope and a compound microscope. 8
a.
b.
9. List three types of specimens a dissecting microscope might be used to view:
a.
b.
Can You Apply What You've Learned?
10. What microscope structures are used to control the amount of light illuminating the specimen?
11. What happened to the light intensity when switching from low to high power?
12. What adjustment will typically have to be made to the light after…
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n
a
4
6.
(c) Photomicrograph (400x).
FIGURE 4.8 (continued)
Post-lab ASSESSMENT 4Bo
7.
6. Structure:
7. Tissue:
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Test I. Identification
Instruction: Refer to the micrographs below in answering the questions.
EST
B
1
3.
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Histopathology techniques
What precaution will you observe in handling of the following specimens?
Hollow organs
Air-filled lungs
Solid organs
Bones and teeth
How do the following factors affect fixation of tissue?
Temperature
Presence of blood
Large tissues
Presence of fats
Presence of mucus
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ble
ru
DUNC
тепон, пратен
2. Complete the labeling activity in FIGURE 4.9 by identifying the tissues and structures indicated.
tc
it
1.
(a)
FIGURE 4.9 Micrographs of tissue samples.
118
LAB EXERCISE 4 Histology
1. Tissue:
- 2.
2. Fiber:
3.
3. Fiber:
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Please answer fast and all
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Instruction: FILL IN THE BLANKS
1. For initial focusing of the specimen the ___________ (objective) is aligned with the body tube and the central opening in the __________.
2. To view the specimen on the stage, secure the slide with the __________. Make sure the slide is in line with the ____________ and the stage opening.
3. Adjust the ______________ to regulate the amount of light required. Adjust the _____________ properly so that optimum light is reflected in the microscope.
4. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray.
5. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed.
6. In shifting to higher magnification, simply…
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i need help asap:)
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please help me make this procedure a flow chart
III. Procedure: A. MicroscopyOperating Procedure:Place the microscope close to the edge of the table. Select a suitable stool so that when looking into the eyepiece, your back is straight and your neck is bent at the nape.1. Lower the body tube by turning the course focus knob until the 10x or 16mm objective reaches the downward stop.2. Look through the eyepiece and adjust the mirror to the position which provides the brightest and the most evenly illuminated field of vision which is the circular area seen in the eyepiece. Raise the condenser until its top lens at the same level as the stage. Place the slide on the stage and fasten it using the stage clips.3. Position the specimen area of the slide over the center of the stage aperture.4. Looking through the eyepiece, raise the coarse focus knob until the image appears. Focus as sharply as possible. Low power objective has a much greater depth of focus and is generally used for the…
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11/2-Try It: Microscopes, Cell Theory, and CellI Pro
An example of a unicellular organism is a
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39. Ticarcillin 700 mg is ordered. Ticarcillin is supplied as a powder in a vial. Directions
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Hue Topic: Discussion Tissues
iscussion_topics/1749440
Multiple Tissues Make Organs
You are a part of a research group who wants to build and organ in the lab for organ transplant patients. If this worked the patients don't have to wait for
donors. The organ you are building requires the following functional abilities:
1. It has to be able to resist mechanical stresses such as friction and abrasion. This tissue will get sloughed off.
2. The ability to contract involuntarily (you don't have to think about it for it to contract) when stimulated by the nervous system.
3. There has to be a tissue that can stimulate the contraction of the other tissue involved.
4. The ability to resist stress, tension and force from many different directions.
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- Please see attached . Thank youarrow_forwardAnatomy and Physiology I Chapter Three The Microscope Exercise 3.5: Determining Depth of Field 6. Referring back to exercise 3.5, record the answers to the questions about colored threads in the spaces provided. 7 Color of bottom thread: Color of top thread: Color of middle thread: * Explalm why proper microscope technique requires always viewing a slide with the scanning objective first before moving to higher-power objectives. Use the concept of depth of field in the explanation. 7 Exercise 3.6: Parts of a Dissecting Microscope 8. Identify two differences between a dissecting microscope and a compound microscope. 8 a. b. 9. List three types of specimens a dissecting microscope might be used to view: a. b. Can You Apply What You've Learned? 10. What microscope structures are used to control the amount of light illuminating the specimen? 11. What happened to the light intensity when switching from low to high power? 12. What adjustment will typically have to be made to the light after…arrow_forwardPlease help make this procedure a flow chartarrow_forward
- What will pose an ethical issue in these procedures are performed - plastic surgery?arrow_forwardTest I. Identification Instruction: Refer to the micrographs below in answering the questions. EST B 1 3.arrow_forwardHistopathology techniques What precaution will you observe in handling of the following specimens? Hollow organs Air-filled lungs Solid organs Bones and teeth How do the following factors affect fixation of tissue? Temperature Presence of blood Large tissues Presence of fats Presence of mucusarrow_forward
- ble ru DUNC тепон, пратен 2. Complete the labeling activity in FIGURE 4.9 by identifying the tissues and structures indicated. tc it 1. (a) FIGURE 4.9 Micrographs of tissue samples. 118 LAB EXERCISE 4 Histology 1. Tissue: - 2. 2. Fiber: 3. 3. Fiber:arrow_forwardPlease answer fast and allarrow_forwardInstruction: FILL IN THE BLANKS 1. For initial focusing of the specimen the ___________ (objective) is aligned with the body tube and the central opening in the __________. 2. To view the specimen on the stage, secure the slide with the __________. Make sure the slide is in line with the ____________ and the stage opening. 3. Adjust the ______________ to regulate the amount of light required. Adjust the _____________ properly so that optimum light is reflected in the microscope. 4. Manipulate the ________________ knob to bring the objective about 1 to 2 mm away from the slide. Never look into the ____________ when bringing the objective close to the slide to avoid damaging or crushing the slide. A clear field of vision should be ___________ not gray. 5. In viewing objects under the microscope, it is observed that the microscope ______________ the direction of movement and _______________ the position of the image observed. 6. In shifting to higher magnification, simply…arrow_forward
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