lab7 lab report

.docx

School

University of Texas, Rio Grande Valley *

*We aren’t endorsed by this school

Course

1102

Subject

Biology

Date

Dec 6, 2023

Type

docx

Pages

3

Uploaded by MateValor11890

Lab #7: Mitosis in Onion Root Tip Date: 11/2/2023 OBJECTIVES To understand the process and stages of mitosis To prepare histological specimens of onion root tips To identify stages of mitosis on the slides INTRODUCTION Quick Overview of cell division In lab 7 Mitosis in Onion Root Tip, my team and I are going to use the microscope to observe Mitosis in an onion cell. In addition, we will see the four stages of mitosis prophase, metaphase, anaphase, and telophase and explain each stage. MATERIALS microscope Onion tips Scissors Forceps 1 M HCl Microscopy slide Cover slip Petri dish Acetocarmine stain Pencil with a eraser Scalpel blade Fish mitosis slide Slide warmer PROCEDURE Part 1. Softening the Roots 1. Cut a 1 cm long onion root tip with a scissors and place in a petri dish. 2. Soak the root tip in a small puddle of 1 M HCl for 5 min. WORK WITH HCL CAREFULLY BECAUSE IT IS A STRONG ACID 3. Staining the root tip. 4. Move the root tip to a microscope slide with forceps. 5. Cut 1 mm of the root tip with a scalpel blade. 6. Cover the root tip with 2 drops of acetocarmine stain and let sit for 5 min. 7. Cover with a cover slip. 8. Push gently down with the eraser of a pencil until the stained tip is spread out, DO NOT TWIST OR PUSH SIDEWAYS. 9. Heat gently (80°C) on a slide warmer for 5 minutes. Part 2. Observing under the Microscope
1. Get an already prepared fish mitosis slide. 2. Set microscope at 10x to scan the slide. 3. Set at 40x for a closer observation. OBSERVATIONS (Please include picture in your lab report) Prophase Metaphase Anaphase Telophase POST-LAB QUESTIONS 1. Many of the cells of the root meristem are not undergoing mitosis, rather they are in a stage called _ Interphase _. Based upon the interpretations made above, interphase appears to be much _ longer _ (shorter / longer) than mitosis. What processes occur in interphase cell prior to the onset of mitosis. 2. How can you distinguish between early and late anaphase? In early anaphase the centromeres divide but in late anaphase the chromosomes reach the poles of the cell. 3. What is a distinguishing visible feature of each stage of mitosis? Prophase During prophase, the chromosomes supercoil, and the
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
  • Access to all documents
  • Unlimited textbook solutions
  • 24/7 expert homework help