LAB 6
.pdf
keyboard_arrow_up
School
Chemeketa Community College *
*We aren’t endorsed by this school
Course
232
Subject
Biology
Date
Dec 6, 2023
Type
Pages
13
Uploaded by LieutenantSquirrel3921
Activity
1:
Microscopes
1-1:
Dissecting
microscopes
Dissecting
microscopes,
also
known
as
stereo
microscopes,
are
used
during
dissections
to
see
a
detailed
view
of
the
organs
revealed
during
a
dissection.
These
microscopes
are
most
useful
for
observing
the
surface
of
specimens
at
a
relatively
low
magnification.
1.
o
Place
the
metric
ruler
on
the
stage
of
the
dissecting
microscope
and
focus
using
the
focusing
knob
on
the
side
of
the
body.
Use
the
magnification
dial
on
the
top
or
the
side
of
the
microscope
to
adjust
to
the
lowest
power
(widest
field
of
view).
What
power
is
the
microscope
at
now?
Record
your
measurement
of
the
field
of
view
diameter
in
mm
in
the
table
below.
Now
adjust
the
magnification
to
the
highest
power
and
measure
the
field
of
view
diameter
in
mm.
'
Convert
the
measurements
to
pum.
1mm=1000um
View
the
letter
“e”
slide
with
the
label
in
the
upright
position.
View
it
in
the
dissecting
microscope
on
the
lowest
power
and
the
highest
power.
Add
drawings
to
the
provided
spaces
below.
Use
the
dissecting
scope
to
view
the
objects
available
at
your
table,
on
your
person
(like
fingers,
license,
dollar
bill),
or
collect
items
from
around
campus
(leaves,
bugs,
flowers)
to
view
under
the
dissecting
microscope.
Questions
1.
Complete
the
table
below.
Magnification
Diameter
(field
of
view)
|
Diameter
(field
of
view)
"
mm
Hm
Di
ting
Mi
LowestPower
|
A0
5
e
00
UM
e
e
L
L
J100n
2.
Draw
the
letter
“e”
in
the
circles
provided
under
section
1-3,
accurately
representing
how
much
of
the
field
of
view
the
“e”
takes
up
at
the
various
magnifications.
1-2:
Compound
Microscope
Overview
Microscopes
are
fragile
and
expensive,
and
we
want
to
maintain
them
in
good
working
order.
Your
instructor
will
walk
you
through
a
tutorial
on
how
to
handle
the
microscope,
understand
its
parts,
and
explain
their
functions.
1
Microscope
Introduction
a.
Caring
for
the
microscope
and
proper
storage
b.
Parts
and
functions
c.
Cleaning
slides
and
lenses,
situating
the
slide
d.
Locating
objects
at
different
magnifications
Label
OQ\)
Make
sure
you
can
identify
the
following
parts
and
explain
what
they
do:
e
Arm
Base
Nosepiecej
Stage
/
Stage
clip
(slide
holder)
¥
Stage
controls
Course
adjustmentj
%
Fine
adjustment
QQ‘(\(\S})‘\CD
Ocular
lenses
(x2)¥
Objective
lenses
(x3)
¥
Condenser
Lamp
/
Brightness
adjusterJ
o5
Questions
1.
What
kinds
of
things
do
you
need
to
be
aware
of
to
make
sure
you
don't
damage
your
microscope?
Weing
14
v
ay
i
¢
pose
2.
When
lookifg
at
a
slide
with*maximum
magnification,
the
objective
lenses
should
always
be
used
in
what
order?
(name
the
lens
and
give
the
magnification)
Led
i
.0
\iQ\\DW
A0
x
Blue
Hdx
3.
What
tools
does
the
microscope
have
to
regulate
how
much
light
passes
through
the
specimen?
bh%h
n
0Y
O\\m
e
oo
d
56}:
4
Questions
.
Complete
the
drawings
of
the
letter
“e”
below.
*"”’\)
e
N
|
K
S
sk
NS
Dlssa;tmg
microscope
Naked
eye
S
L
e
40x
total
100x
total
|
400x
total
magnification
magnification
magnification
2.
What
happens
to
the
orientation
(not
the
magnification)
of
the
letter
“e”
when
it
is
viewed
in
the
compound
microscope
as
opposed
to
with
the
naked
eye?
11
oecornes
)
ppe
cl
1-4
Magnification,
field
of
view,
and
depth
of
field
You
have
already
examined
several
differences
in
magnification
by
looking
at
the
letter
when
you
increase
the
magnification
of
an
object,
you
can
see
less
of
that
object.
Or,
in
a
more
scientific
term,
your
field
of
view
decreases
as
magnification
increases.
Also
notice
that
when
you
use
a
lower
magnification,
you
can
see
most
of
the
depth
of
the
object
in
focus
at
the
same
time.
When
magnification
is
increased,
only
smaller
depths
of
the
object
can
be
focused
on
at
the
same
time.
This
means
that
the
depth
of
field
is
decreased
when
magnification
increases.
We'll
explore
these
phenomena
further
with
this
activity.
‘e”.
You
have
seen
that
1.
Observe
the
magnification
marked
on
the
eyepiece
and
each
of
the
three
objective
lenses.
Record
these
values
in
the
table
below.
Calculate
the total
magnification
by
multiplying
the
objective
magnification
times
the
ocular
magnification.
2.
To
measure
the
field
of
view:
use
the
4x
(scanning)
objective.
Place
a
clear
metric
ruler
under
the
stage
clip
so
you can see
the
markings
on
the
metric
side
of
the
ruler
and
measure
the
diameter
of
the
field
of
view
in
mm.
Record
the
value
in
the
table
below.
Now
measure
the
field
of
view
with
the
10x
objective
and
record
that
value
as
well.
You
will
not
be
able
to
directly
measure
the
field
of
view
for
the
40x
objective.
_
To
calculate
the
field
of
view
for
the
40x
objective,
we
can use
this
ratio
between
magnification
and
field
of
view
diameter:
)
Lower
power
maghification
Higher
power
diameter
Higher
power
magnification
|
gwer
power
diameter
A
For
example,
if
we
measured
the
10x
field
of
view
diameter
to
be
3mm,
we
could
solve
for
the
40x
field
of
view
diameter
as
shown
below:
10x
High
power
diameter
40x
Simplify
this
.~
fraction
3%rim
N
3mm
x
0.25
=
high
power
diameter
0.75mm
=
high
power
diameter
Muiiiply
both
sides
by
3mm
4.
When
usihg
compound
microscopes,
it's
more
typical
to
use
micrometers
(um)
to
measure
objects.
Convert
the
field
of
view
diameter
to
pm.
5.
To
observe
how
depth
of
field
changes,
we
will
use
the
crossed-threads
slide.
Use
the
4x
objective
to
focus
on
the
point
where
the
three
threads
cross.
Can
you
see
all
three
threads
clearly?
Refocus
on
this
point
using
the
10x
and
the
40x
objectives.
Are
all
three
threads
still
equally
clear?
Answer
the
questions
below
about
the
depth
of
field.
Questions
1.
Complete
the
table
below:
Name
of
lens
Magnification
Diameter
(field
|
Diameter
(field
of
view)
mm
of
view)
pm
Objective
Ocular
Total
Scanning
A
LO
¢
“0
0
00|
HODOKM
Lowpover__|
10
0,
[109
_
[7.00mm
[2000
™
High
power
V\O
{O)g
4*100
O“@W\m
60@
\/\YY\
:
A—
2.
Without
using
a
ruler,
how
could
you use
the
above
information
to
figure
out
how
big
in
pm
the
“e”
is
from
the
previous
activity?
Use
your
drawmgs
to
ewte
the
snze
of
the
letter
“e”
jn
Compmre,
e
€
YYACA
v
e
|
k
3.
Is
it
‘as
clear
to
focus
on
all
three
threads
at
the
400x
total
magnification
as
it
is
at
the
40x
total
magnification?
If
not,
why?
/Ar\’
\o
0+
\{\
A
’/f
O
X
€
O
X,
o
Comn
u.z
oNn
1
\(\é/
+ho:
»eigig
oA
o
HODx
1ts
W‘\Ove
ddfr@&“
Pocosed
4.
What
is
the
order
of
the
colored
threads
on
the
slide?
Top
v
€c
Middle
|
,Ulow
Bottom
0|
v
€
5.
What
can
you
conclude
about
the
size
of
the
field
of
view
in
relation
to
magnification?
e
field
view
g
solley
e
Wigheye
g
MOAANA
caa
0N
ety
6.
What'can
you
conc
ude
about
the
depth
of
field
in
relation
to
magnification?
}
0S
mO\
™
Hecahon
cjoes
VP
|
\(éQ,
N
ACYEeas
e
_
Activity
Observmg
prepared
and
live
specimens
It
is
helpful
to
observe
fresh
specimens
in
order
to
view
things
that
only
happen
in
living
cells.
Some
cells
move
through
their
environment.
Others
show
a
cycling
of
their
cytoplasm.
Still
others
show
metabolic
changes,
particularly
if
effective
dyes
or
indicators
are
used.
“Prepared”
slides
that
have
been
stored
in
a
cabinet
cannot
show
such
behaviors.
Prepared
slides
are
useful
when
studying
the
structures
of
organisms
or
tissues.
Keep
in
mind
that
when
looking
at
prepared
slides,
these
are
often
stained
in
order
to
make
the
cells
or
specimen
visible.
The
color
something
appears
in
a
prepared
slide
is
not
always
its
true
color
if
it
were
living.
Most
cells
are
small
and
must
be
magnified
100x
or
more
to
be
clearly
seen.
Additionally,
it
may
be
useful
to
use
dyes
or
stains
when
looking
at
live
specimens,
otherwise
the
cells
appear
as
gray
blobs.
Specific
dyes
are
absorbed
by
certain
parts
of
the
cell.
For
instance,
the
cell
nucleus
often
stains
a
dark
color.
This
isn’t
because
the
nucleus
is
darker
than
the
rest
of
the
cell,
it
just
absorbs
more
of
the
stain,
making
it
more
visible.
It
is
also
helpful
to
regulate
the
light
when
looking
at
both
live
and
prepared
specimens.
Use
the
light
adjustment
knob
and
the
condenser
knob
to
change
the
amount
of
light
that
comes
through
and
the
contrast
of
the
image.
This
will
make
parts
of
the
cell,
like
the
cell
wall,
stand
out.
Cells
Cells
are
the
simplest
individual
units
of
life,
and
humans
are
made
of
several
trillion
of
them
working
together
-
to
create
a
functional
organism.
You
will
need
to
understand
the
structure
of
cells
and
functions
of
organelles
forever,
so
learn
them
now.
There
are
two
major
categories
of
cells:
prokaryotic
cells
like
bacteria
are
relatively
small
with
a
simpler
internal
structure,
and
gukaryotic
cells
which
comprise
protozoa,
animal,
plant
and
fungal
cells.
Eukaryotic
cells
are
much
larger
and
contain
nuclei
and
membrane-bound
organelles.
Cells
come
in
a
surprising
variety
of
shapes
and
sizes
and
carry
out
very
diverse
functions,
yet
are
all
made
of
the
same
core
parts:
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Questions
SBI 3C1
VIRTUAL LAB: THE MICROSCOPE
INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the
instructions on how to properly use a microscope. When you are complete, answer the questions below.
PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen?
TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification)
Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE
ОBJECTIVE
LENS
POWER
OCULAR
MAGNIFICATION
OBJECTIVE LENS
MAGNIFICATION
TOTAL MAGNIFICATION
MAG (X) = Ocular X Objective
LOW
LP MAG =
MEDIUM
MP MAG=
HIGH
HP MAG-
Complete the following chart by calculating the missing lens or total magnification [2]
TOTAL MAGNIFICATION
OBJECTIVE LENS MAGNIR
AR (EYEPIECE)
MAGNIFICATION
5X
80X
10X
40X
10X
100X
500X
50X
PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES
Access the Virtual Microscope at…
arrow_forward
Match the parts of the microscope with its job.
arrow_forward
A. Purpose:
Figure 1
B. Materials:
Microscope
Magazine
Slides and cover slips
Paper towels
Pipette
Scissors
C. Procedure:
1. Careful carry a microscope to your lab area. Make sure to hold it with one
hand under the base and one hand on the arm as shown in Figure 1.
2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look
at your ocular lens. What is the magnification of the ocular lens (eye piece)?
Figure 2
3. Fill in the chart to show the total magnification for each objective lens.
Magnification
of Ocular Lens
Magnification of
Objective Lens
Objective Lens
Total
Magnification
Low Power
Medium Power
High Power
arrow_forward
9:06
LTE
4) Answer true or false to each of the following statements.
On high power, you should use the coarse
adjustment knob.
The diaphragm determines how much light shines on
the specimen.
The low power objective has a greater magnification
than the scanning objective.
The fine focus knob visibly moves the stage up and
down.
Images viewed in the microscope will appear upside
down.
If a slide is thick, only parts of the specimen may
come into focus.
The type of microscope you are using is a scanning
microscope.
For viewing, microscope slides should be placed on
the objective.
In order to switch from low to high power, you must
rotate the revolving nosepiece.
The total magnification of a microscope is
determined by adding the ocular lens power to the
objective lens power.
arrow_forward
1. Identify features of Dark-field Microscopy (1-2 paragraph)
2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)
arrow_forward
RESULTS for Exercise 4: Microscopic Measurements
Name
Date
Calibration of Microscope:
PART A
1 Division on the Ocular Micrometer
corresponds to:
Objective
Scanning
Low Power
μη
High Dry
um
Oil Immersion
um
Show calculations for each objective:
Exercise 4 Microscopic Measurements 35
arrow_forward
Direction: Read and analyze the following laboratory experiment and answer the
following question.
PART 3: PLASMOLYSIS
Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps,
compound microscope, iodine, small knife, water, salt (5% and 10% solution)
Methodology:
1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife.
2. Use an eye dropper to place a drop of water in the center of a microscope slide.
Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion
wedge and place it in the center of the microscope slide.
3.
4.
5.
Add a drop of water and a drop of iodine over the onion tissue on the slide.
Carefully lower a cover glass slip at an angle on the stained tissue to allow air
bubbles to escape.
6.
Examine the prepared slide under the compound microscope at 100X
magnification.
7. Record what the cells look like.
8.
Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per
100…
arrow_forward
1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity
2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate:
i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions.
3. What are the methods of staining?
arrow_forward
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step.
1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage.
a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage.
4. Use the Low Power Objective.
a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
arrow_forward
answer the following:
instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:
arrow_forward
The specimen you are examining is very thin and transparent. What can you do to make it easier to see
arrow_forward
Please complete resolution and magnification
arrow_forward
Which of the following microscopy
techniques can be used in live cell imaging
in a live animal.
1. Structured illumination microscopy
2. Stimulated emission depletion
microscopy
3. Photoactivated localization microscopy
4. Two-photon excitation microscopy
5. Total internal reflection microscopy
6. Light-sheet microscopy
O4 and 6
O2, 3 and 6
O1 and 4
3 and 5
O2 and 4
arrow_forward
3.
Please number these steps, in the order they should be completed when using a microscope.
Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf.
Move to other objective lenses to increase magnification.
Rotate lowest-magnification objective lens into position (probably 4x or 10x).
Clean all lenses with lens paper.
Place specimen on stage.
Adjust chair to a comfortable height.
Use coarse focus knob to adjust stage until an image is visible.
Be careful to use only the fine focus knob with the longest objective lenses.
arrow_forward
Direction: Read and analyze the following laboratory experiment and answer the
following question.
PART 1: SURFACE AREA AND CELL SIZE
Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein
cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels.
Methodology:
1. Safety: Wear goggles and nitrile gloves while completing this lab.
2. Obtain three different size blocks of pink or blue agar. Using a ruler,
measure the length, width, and height of the three blocks given below. Cut
the agar according to the given dimension.
Small = 1 cm x 1 cm x 1 cm
Medium = 2 cm x 2 cm x 2 cm
•
• Large = 1 cm x 1 cm x 6 cm
3. Record your data.
4.
Pour HCl or vinegar into two small cups. Place the one larger "cell" into one
cup and the two smaller cells in the other cup. Start timing 30 minutes.
5. After 30 minutes, remove the cells and blot them dry with a paper towel.
6. Using your ruler, measure the distance the HCl has diffused into the blocks
as shown on the…
arrow_forward
II.VIRTUAL MICOSCOPE
4X
4X
SPECIMEN
10X
10X
40X
40X
100X
100x
COARS FOCUA
TME FOOR
LCHT AVAN
100X
MAGNIFICATIO | 4X
N USED IN THE
VIRTUAL
MICROSCOPE
OCULAR
MAGNIFICATIO
(10X)
TOTAL
MAGNIFICATIO
arrow_forward
Please answer the following questions for Part C
arrow_forward
Fill out the chart
arrow_forward
QUESTIONS TO ANSWER:
I. Basic Microscopy
1. State 3 ways to handle the microscope properly.
arrow_forward
Write the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.
arrow_forward
Answer these questions by watching the YouTube videos and reviewing the Powerpoints from Lab #4.
1. What is Refraction of light?
2. What is the difference between the Ocular lens and the Objective lens?
3. What is the purpose of the Revolving Nosepiece?
4. What is the difference between the Course Adjustment Knob and the Fine Adjustment Knob?
5. How do you calculate Total Magnification?
6. What is Resolution in terms of Microscopy?
7. What is the purpose of Oil Immersion?
8. What is the Diffraction Barrier and why does it exist?
9. What is the purpose of using stains and fluorescent dyes in microscopy?
10. What is the advantage of using an Electron Microscope?
11. What Objective lens should you always start with?
12. What is the purpose of the Iris Diaphragm on the Condenser?
13. How do you know your Objective lens has been adjusted properly?
14. Why should you not use Kimwipes…
arrow_forward
3
6
9
12
V
Stage control knob
1.
Mechanical support - is used for
carrying the microscope
Fine adjustment knob
2.
Platform upon which specimen is
placed
Objective
3.
Brings object into approximate focus
Diaphragm/Iris
4.
Eyepiece/ocular
5.
Collects light from source and focuses
it upon specimen
Controls amount of illumination used
to view the object; impacts contrast
Stage
nation
Base
6.
Lenses you look into
7.
Provides more precise focusing on the
object
8.
Revolving device that holds the
Condenser
objective lenses
9.
Provides additional magnification of
Arm
varying powers
10.
Mechanical support rests on flat
Nosepiece
Coarse adjustment knob
Stage clip
surface of table
11. Hold slides in place
12.
122
Provides mechanical means to move
the stage
arrow_forward
Question:-
What is the best superresolution microscopy method to resolve in z direction.
arrow_forward
Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
arrow_forward
PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_
The field of view (FOV) is the circular area you can see when you look through the microscope. The
diameter of the field of view is different depending on which objective lens you are using. For example,
you are using the medium-power objective lens, then the area you can see is actually smaller than if you
were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate
actual size of objects / cells seen through the microscope.
When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1).
Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left
edge of the field of view (see figure 2).
NOTE:
Slage cip
I-1000
Objects in the FOV are usually
measured in micrometers (um).
To convert, a FOV in mm, times
it by 1000…
arrow_forward
Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?
arrow_forward
PROCEDURE:
1. Set two pencils down parallel from each other. Make them about 2-3 inches apart as the length of your slides to keep
things easy.
2. Stick a long piece of tape over the
two pencils and to the table on either
side of the pencils to hold the tape
tightly between the two pencils like
a bridge.
3. Don’t touch the sticky side of the
tape or you will ruin the microscope.
Drop a small drop of water onto the
top of the tape using the pipette or
medicine dropper.
4. Make 3-4 lines of tape and add a
different-sized drop to each one.
This will help determine what size
of water droplet produces the biggest
magnification.
5. Prepare a rectangular shape of plastic cover. Put a small slice of onion.
Slide the rectangular shape of the plastic cover with the small slice of onion under the pieces of tape and observe the size
of the onion on different droplets. Write your observations on the table below.
No. of drops
Observation
arrow_forward
Answer the following questions and uploa
assignment.
1. What is the difference between a zoom knob and focus knob?
2. What is another name for the ocular lens?
3. Define working distance.
4. When would you use top illumination vs. bottom illumination?
5. What two parts of the microscope should you touch when carrying it?
MacBook Air
吕0
F3
F4
F5
F6
F7
arrow_forward
30
What is the magnification of the microscope shown below? Grid your answer.
10x
O 000
25x
arrow_forward
1. Match: Read about each microscope part. Match the description to the part on the diagram.
Stage: Platform where a slide is placed.
Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly
magnifies a sample by 10x.
Coarse focus knob: Large knob that moves the stage up and down to focus the sample.
Fine focus knob: Small knob that moves the stage over a short distance to refine the focus.
Objective lens: A second lens that further magnifies the sample. Microscopes usually have several
objective lenses with different magnifications.
Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.
arrow_forward
Using the microscope Answer the following questions as you work through this exercise:
1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)?
2. How is the letter “e” on the slide oriented when you see it under low or high power magnification?
3. What effect, if any, does the compound light microscope have on the orientation of the image?
arrow_forward
I have a hard time with this picture and I don't know which I put the right label on this picture. Can you help me?
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Related Questions
- SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…arrow_forwardMatch the parts of the microscope with its job.arrow_forwardA. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Powerarrow_forward
- 9:06 LTE 4) Answer true or false to each of the following statements. On high power, you should use the coarse adjustment knob. The diaphragm determines how much light shines on the specimen. The low power objective has a greater magnification than the scanning objective. The fine focus knob visibly moves the stage up and down. Images viewed in the microscope will appear upside down. If a slide is thick, only parts of the specimen may come into focus. The type of microscope you are using is a scanning microscope. For viewing, microscope slides should be placed on the objective. In order to switch from low to high power, you must rotate the revolving nosepiece. The total magnification of a microscope is determined by adding the ocular lens power to the objective lens power.arrow_forward1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)arrow_forwardRESULTS for Exercise 4: Microscopic Measurements Name Date Calibration of Microscope: PART A 1 Division on the Ocular Micrometer corresponds to: Objective Scanning Low Power μη High Dry um Oil Immersion um Show calculations for each objective: Exercise 4 Microscopic Measurements 35arrow_forward
- Direction: Read and analyze the following laboratory experiment and answer the following question. PART 3: PLASMOLYSIS Materials: safety goggles, red onion, dropper, slides & cover slips, tweezers/ forceps, compound microscope, iodine, small knife, water, salt (5% and 10% solution) Methodology: 1. With goggles on, carefully cut the onion into wedge shaped pieces using a knife. 2. Use an eye dropper to place a drop of water in the center of a microscope slide. Use the tweezers to peel a thin layer of skin tissue from the thick part of the onion wedge and place it in the center of the microscope slide. 3. 4. 5. Add a drop of water and a drop of iodine over the onion tissue on the slide. Carefully lower a cover glass slip at an angle on the stained tissue to allow air bubbles to escape. 6. Examine the prepared slide under the compound microscope at 100X magnification. 7. Record what the cells look like. 8. Prepare a 5% salt solution by adding 5 grams of salt (measure with balance) per 100…arrow_forward1. How will the following affect resolution during microscopy?i. Closing or opening the diaphragmii. Raising or lowering the condenserii. Increasing or reducing the light intensity 2. During the calibration of an ocular micrometer, two (2) stage micrometer divisions were observed to line up with ten (10). If the graduation interval on the stage micrometer is 0.01mm, calculate: i. The calibrated value of the ocular micrometer in um.ii. The size of Pseudomonas aeruginosa which has occupied 5 ocular divisions. 3. What are the methods of staining?arrow_forwardList down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…arrow_forward
- answer the following: instruction.match the name of the major part (listed below) with its location on the microscope, and give a very brief description of what each is used for:arrow_forwardThe specimen you are examining is very thin and transparent. What can you do to make it easier to seearrow_forwardPlease complete resolution and magnificationarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education