Chapter 12 Answers-Transport

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Chapter 12 Membrane Transport 1: Which of the following channels would not be expected to generate a change in voltage by movement of its substrate across the membrane where it is found? a. an aquaporin b. a sodium channel c. a calcium channel d. a proton channel ANS: A Aquaporin channels are found in the plasma membrane of some cells, where they facilitate the diffusion of water across the membrane. Because water is an uncharged molecule, its movement would not be expected to alter the voltage across the membrane. DIF: Easy REF: 12.1 OBJ: 12.1.k Describe the ways in which water can move across cell membranes and articulate what governs whether water will enter or exit a cell. MSC: Understanding 2: Below is a list of molecules with different chemical characteristics. Knowing that all molecules will eventually diffuse across a phospholipid bilayer, select the option below that most accurately predicts the relative rates of diffusion of these molecules (fastest to slowest). alanine estrogenpropanolsodium a. alanine > propanol > sodium > estrogen b. sodium > propanol > alanine > estrogen c. estrogen > propanol > sodium > alanine d. estrogen > propanol > alanine > sodium ANS: D Estrogen is a steroid hormone and will diffuse the fastest across the membrane. Propanol is a small, uncharged molecule with a polar group. Alanine is an amino acid, and although it has a small, nonpolar side group, amino acids are charged molecules. Sodium is an ion and will move the slowest across the bilayer. DIF: Easy REF: 12.1 OBJ: 12.1.c Review the properties that govern the rate at which a given solute can cross a protein-free lipid bilayer. MSC: Understanding
3: Cells use membranes to help maintain set ranges of ion concentrations inside and outside the cell. Which of the following ions is the most abundant inside a typical mammalian cell? a. Na + b. K + c. Ca 2+ d. Cl ANS: B DIF: Easy REF: 12.1 OBJ: 12.1.e Indicate how the concentration of ions in the cell differs from that outside the cell. MSC: Remembering 4: Cells use membranes to help maintain set ranges of ion concentrations inside and outside the cell. Which of the following ions is the most abundant outside a typical mammalian cell? a. Na + b. K + c. Ca 2+ d. Cl ANS: A DIF: Easy REF: 12.1 OBJ: 12.1.e Indicate how the concentration of ions in the cell differs from that outside the cell. MSC: Remembering 5: Which of the following statements about resting membrane potential is true? a. The resting membrane potential for most animal cells is 0 mV, because the positive and negative ions are in balance. b. The resting membrane potential for most animal cells is positive, because Na + ions are so plentiful inside cells. c. The resting membrane potential for most animal cells is negative, because the inside of the cell is more negatively charged than the outside of the cell. d. At the resting membrane potential, no ions enter or exit the cell. ANS: C DIF: Easy REF: 12.1 OBJ: 12.1.f Define “resting membrane potential.” MSC: Understanding
6: A hungry yeast cell lands in a vat of grape juice and begins to feast on the sugars there, producing carbon dioxide and ethanol in the process: C 6 H 12 O 6 + 2ADP + 2P i + H + 2CO 2 + 2CH 3 CH 2 OH + 2ATP + 2H 2 O Unfortunately, the grape juice is contaminated with proteases that attack some of the transport proteins in the yeast cell membrane, and the yeast cell dies. Which of the following is the most likely cause of the yeast cell’s demise? a. toxic buildup of carbon dioxide inside the cell b. toxic buildup of ethanol inside the cell c. diffusion of ATP out of the cell d. inability to import sugar into the cell ANS: D DIF: Moderate REF: 12.1 OBJ: 12.1.g Compare how transporters and channels discriminate among solutes, moving only a select subset across the membrane. MSC: Applying 7: Some cells express aquaporin proteins—they are channel proteins that facilitate the flow of water molecules through the plasma membrane. What regulates the rate and direction of water diffusion across the membrane? a. aquaporin conformation b. resting membrane potential c. solute concentrations on either side of the membrane d. availability of ATP ANS: C DIF: Easy REF: 12.1 OBJ: 12.1.k Describe the ways in which water can move across cell membranes and articulate what governs whether water will enter or exit a cell. MSC: Remembering 8: Pumps are transporters that are able to harness energy provided by other components in the cells to drive the movement of solutes across membranes, against their concentration gradient. This type of transport is called a. active transport. b. free diffusion.
c. facilitated diffusion. d. passive transport. ANS: A DIF: Easy REF: 12.1 OBJ: 12.1.h Distinguish between active and passive transport and indicate which type of membrane transport protein carries out each. MSC: Remembering 9: You have generated antibodies that recognize the extracellular domain of the Ca 2+ -pump. Adding these antibodies to animal cells blocks the active transport of Ca 2+ from the cytosol into the extracellular environment. What do you expect to observe with respect to intracellular Ca 2+ ? a. Ca 2+ -pumps in vesicle membranes keep cytosolic calcium levels low. b. Ca 2+ -pumps in the endoplasmic reticulum membrane keep cytosolic calcium levels low. c. Ca 2+ -pumps in the Golgi apparatus keep cytosolic calcium levels low. d. Ca 2+ concentrations in the cytosol increase at a steady rate. ANS: B In addition to the Ca 2+ -pumps in the plasma membrane, Ca 2+ -pumps are also found in the membrane of the endoplasmic reticulum (ER). Those in the ER membrane will continue to remove calcium ions from the cytosol, keeping calcium levels low. DIF: Moderate REF: 12.2 OBJ: 12.2.e Explain why—and how—cells keep the cytosolic concentration of calcium ions low. MSC: Applying 10: Cells make use of H + electrochemical gradients in many ways. Which of the following proton transporters is used to regulate pH in animal cells? a. light-driven pump b. H + ATPase c. H + symporter d. Na + -H + exchanger ANS: D The high extracellular concentration of Na + is employed by a transporter that pumps protons out of animal cells as Na + is brought into the cell. The other transporters are found only in bacterial cells.
DIF: Easy REF: 12.2 OBJ: 12.2.h Express how the sodium–proton exchanger in the plasma membrane allows animal cells to control the pH of their cytosol. MSC: Remembering 11: Ca 2+ -pumps in the plasma membrane and endoplasmic reticulum are important for a. maintaining osmotic balance. b. preventing Ca 2+ from altering the activity of molecules in the cytosol. c. providing enzymes in the endoplasmic reticulum with Ca 2+ ions that are necessary for their catalytic activity. d. maintaining a negative membrane potential. ANS: B The major purpose of the Ca 2+ -pumps is to keep the cytosolic concentration of Ca 2+ low. When Ca 2+ does move into the cytosol, it alters the activity of many proteins; hence, Ca 2+ is a powerful signaling molecule. DIF: Easy REF: 12;2 OBJ: 12.2.e Explain why—and how—cells keep the cytosolic concentration of calcium ions low. MSC: Remembering 12: Which of the following occur WITHOUT coupling transport of the solute to the movement of a second solute? a. import of glucose into gut epithelial cells b. export of Ca 2+ from the cytosol c. export of H + from animal cells for pH regulation d. the export of Na + from cells to maintain resting membrane potential ANS: B Ca 2+ is exported using ATP-powered pumps. There are no other solutes that are being moved by these pumps. DIF: Easy REF: 12.2 OBJ: 12.2.a Outline how the glucose transporter in the plasma membrane of mammalian cells imports glucose after a meal and exports glucose to provide fuel for other tissues in the body. | 12.2.e Explain why—and how—cells keep the cytosolic concentration of calcium ions low. | 12.2.h Express how the sodium–proton exchanger in the plasma membrane allows animal cells to control the pH of their cytosol. MSC: Understanding
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