BIOL 1107L Starch Analysis Results (2)

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1107L

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Biology

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Apr 3, 2024

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BIOL 1107L Starch Analysis Results Your GLA has presented and explained about how a standard curve can be used in a quantitative analysis. (Questions are worth 2 pts each) Standard Curve 1. Briefly describe your experimental design. Include what your known concentrations will be and how you will create them. Five solutions were created with the starch stock concentration given, and Lugol’s reagent. (0.1 g/L) We did this so the spectrophotometer can calculate the absorbance. A negative control was made with a concentration of 0.0 g/L. We then made four more solutions with concentrations of .2 g/L, .4 g/L, .6 g/L, and .8 g/L. We then measured the absorbance of the five solutions at wavelength 580nm and we created a standard curve of the data. Then, with the three supplements, we created a solution with 4mL of the supplement and 1 mL of Lugol’s Reagent. We measured the absorbance of these substances using wavelength 580nm again, and we put these data points onto the standard curve to find the concentration of the solutions. 2. Using the spectrophotometer, find the absorbance values for your concentrations and fill out your table below. Starch Concentration Absorbance 0.2 .275 0.4 .560 0.6 .871 0.8 1.0 1.133 1.365 3. Using Excel, create a standard curve graph and copy the image and the slope equation below.

BIOL 1107L Starch Analysis Results Starch Analysis 4. Fill in the table below with the absorbance values obtained for your samples. Add additional lines as needed. Sample Absorbance Supplement A 1.026 Supplement B .651 Supplement C .382 5. Fill in the table below with the starch concentrations calculated for your samples, include appropriate units. Add additional lines as needed. Sample Starch Concentration Supplement A 0.7 Supplement B 0.5 Supplement C 0.3

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