Effects of temperature, oxygen, and chemical disinfectants on microbial growth_revised(1)
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Effects of temperature, oxygen, and chemical disinfectants on microbes
Part I: Effects of temperature
1.
Go over information in the following link (
effects of temperature link
) and define the following terms: Psychrophiles – cold-loving microorganisms that can grow at 0C and below
Psychrotrophs – organisms that prefer cooler temperatures and can grow between 4C – 25C
Mesophiles – middle-loving organisms that can grow between 20C – 45C
Thermophiles – heat-loving organisms that grow at optimal temperatures between 50C – 80C
Hyperthermophiles – extreme heat-loving organisms that grow at optimal temperatures of 80C-110C
0ptimal temperature. – Range of temperature at which growth rates are highest.
2. Microbiology, like most sciences, works in celcius. As Americans, many of us are not used to thinking about temperatures given in celcius. Using a Fahrenheit to Celsius calculator (
LINK
), convert the following temperatures (in celcius) to Fahrenheit. Label the boiling point of water, the freezing point of water, the average body temperature, and room temperature (choose the one closest to room temperature).
0C = 32F – Freezing point of water
23C = 73.4F – Room temperature
30C = 86F
37C = 98.6F – Body temperature
100C = 212F
- Boiling point of water
3. You perform an experiment with three different bacteria (A, B, C) to determine how they grow at different temperatures. The results are displayed in the table below. ‘-‘ means no growth; ‘+’ means slight amount of growth; ‘++’ means moderate amount of growth; and ‘+++’ means heavy growth. For each bacteria, determine whether they are psychrophiles, psychrotrophs, mesophiles, or thermophiles. Explain your reasoning.
Bacteri
a
4C
refrigerator
10
C
25C
37C
55C
A
-
-
+
+++
-
Bacteri
a
4C
refrigerator
10
C
25C
37C
55C
B
+
+
+++
-
-
C
-
-
-
-
+++
Results:
A: Mesophile
B: psychrophile
C: thermophile
5. You are studying the effect of temperature on four bacterial species: Escherichia coli
(
E. coli
), Bacillus stearothermophilus
(
B. stearo
), Pseudomonas fluorescens
(
P. fluorescens
or P. fluoro
), and Bacillus subtilis
(
B. subtilis
). Your results are shown in the image below. (Images taken from https://thevirtualedge.weebly.com/experiment-14.html).
Analyze the results and fill in the table using the table in #4 as an example. Determine the type of microbe (psychrophile, psychrotroph, mesophile, or thermophile)
Bacterial species
4C
22
C
37C
55
C
Type of microbe E. coli
-
++
+++
+
Mesophile
P. fluorescens
+
+++
+
-
Mesophile
B. subtilis
-
++
+++
+
Mesophile
B. stearothermophilus
-
-
-
+++
Thermophile
6. In your own words, explain why refrigeration and cooking are so important to food safety. Refrigeration and cooking are important to food safety because they help prevent the growth of harmful bacteria. Refrigeration slows down the growth and cooking to a certain temperature kills any
bacteria that might be present. 7. Based on what you’ve learned and using the terminology in question #1, most human pathogens would be _
mesophiles
_______.
8. Use Google to determine long can cooked food safely stay at room temperature? Using everything you’ve learned, why do you think this is? Cooked food should not stay out longer than two hours because bacteria (mesophiles) will start to grow rapidly in their optimal temperature (room temp).
9. Do some research in Google and determine the following temperatures. Using this Fahrenheit to Celsius calculator (
LINK
), give your answers in both Fahrenheit and Celsius.
Optimal refrigeration temperature: 37F – 2.77C
Internal temperature to target for cooking ground beef: 160F – 71.11C
Internal temperature to target for cooking raw chicken: 165F – 73.88C
Internal temperature to target for cooking raw pork: 145F – 62.77C
Part 2: Aerotolerance
Watch the following video (
LINK
) and go over the module 4 powerpoint (slides 37-41) to answer the questions below. The video is very important here. You will not be able to answer all these questions with just the powerpoint and book.
1.
Define the following:
Aerotolerance – Ability to tolerate oxygen, but not required
obligate aerobe – requires oxygen at normal levels, 20-21%
obligate anaerobe – cannot tolerate oxygen
facultative anaerobe – can grow with or without oxygen but prefers it to use for aerobic respiration
aerotolerant anaerobe – Do not require oxygen but can tolerate it. Cannot utilize oxygen
microaerophile. – require oxygen but in lower concentrations, 2-10%
2. What is the name of the media that allows us to determine the aerotolerance of a microbe? Fluid Thioglycollate Media (FTM)
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