Effects of temperature, oxygen, and chemical disinfectants on microbial growth_revised(1)

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Rowan-Cabarrus Community College *

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Biology

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Apr 3, 2024

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docx

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Effects of temperature, oxygen, and chemical disinfectants on microbes Part I: Effects of temperature 1. Go over information in the following link ( effects of temperature link ) and define the following terms: Psychrophiles – cold-loving microorganisms that can grow at 0C and below Psychrotrophs – organisms that prefer cooler temperatures and can grow between 4C – 25C Mesophiles – middle-loving organisms that can grow between 20C – 45C Thermophiles – heat-loving organisms that grow at optimal temperatures between 50C – 80C Hyperthermophiles – extreme heat-loving organisms that grow at optimal temperatures of 80C-110C 0ptimal temperature. – Range of temperature at which growth rates are highest. 2. Microbiology, like most sciences, works in celcius. As Americans, many of us are not used to thinking about temperatures given in celcius. Using a Fahrenheit to Celsius calculator ( LINK ), convert the following temperatures (in celcius) to Fahrenheit. Label the boiling point of water, the freezing point of water, the average body temperature, and room temperature (choose the one closest to room temperature). 0C = 32F – Freezing point of water 23C = 73.4F – Room temperature 30C = 86F 37C = 98.6F – Body temperature 100C = 212F - Boiling point of water 3. You perform an experiment with three different bacteria (A, B, C) to determine how they grow at different temperatures. The results are displayed in the table below. ‘-‘ means no growth; ‘+’ means slight amount of growth; ‘++’ means moderate amount of growth; and ‘+++’ means heavy growth. For each bacteria, determine whether they are psychrophiles, psychrotrophs, mesophiles, or thermophiles. Explain your reasoning. Bacteri a 4C refrigerator 10 C 25C 37C 55C A - - + +++ -
Bacteri a 4C refrigerator 10 C 25C 37C 55C B + + +++ - - C - - - - +++ Results: A: Mesophile B: psychrophile C: thermophile 5. You are studying the effect of temperature on four bacterial species: Escherichia coli ( E. coli ), Bacillus stearothermophilus ( B. stearo ), Pseudomonas fluorescens ( P. fluorescens or P. fluoro ), and Bacillus subtilis ( B. subtilis ). Your results are shown in the image below. (Images taken from https://thevirtualedge.weebly.com/experiment-14.html). Analyze the results and fill in the table using the table in #4 as an example. Determine the type of microbe (psychrophile, psychrotroph, mesophile, or thermophile)
Bacterial species 4C 22 C 37C 55 C Type of microbe E. coli - ++ +++ + Mesophile P. fluorescens + +++ + - Mesophile B. subtilis - ++ +++ + Mesophile B. stearothermophilus - - - +++ Thermophile 6. In your own words, explain why refrigeration and cooking are so important to food safety. Refrigeration and cooking are important to food safety because they help prevent the growth of harmful bacteria. Refrigeration slows down the growth and cooking to a certain temperature kills any bacteria that might be present. 7. Based on what you’ve learned and using the terminology in question #1, most human pathogens would be _ mesophiles _______. 8. Use Google to determine long can cooked food safely stay at room temperature? Using everything you’ve learned, why do you think this is? Cooked food should not stay out longer than two hours because bacteria (mesophiles) will start to grow rapidly in their optimal temperature (room temp). 9. Do some research in Google and determine the following temperatures. Using this Fahrenheit to Celsius calculator ( LINK ), give your answers in both Fahrenheit and Celsius. Optimal refrigeration temperature: 37F – 2.77C Internal temperature to target for cooking ground beef: 160F – 71.11C Internal temperature to target for cooking raw chicken: 165F – 73.88C Internal temperature to target for cooking raw pork: 145F – 62.77C Part 2: Aerotolerance Watch the following video ( LINK ) and go over the module 4 powerpoint (slides 37-41) to answer the questions below. The video is very important here. You will not be able to answer all these questions with just the powerpoint and book. 1. Define the following: Aerotolerance – Ability to tolerate oxygen, but not required obligate aerobe – requires oxygen at normal levels, 20-21% obligate anaerobe – cannot tolerate oxygen facultative anaerobe – can grow with or without oxygen but prefers it to use for aerobic respiration aerotolerant anaerobe – Do not require oxygen but can tolerate it. Cannot utilize oxygen microaerophile. – require oxygen but in lower concentrations, 2-10% 2. What is the name of the media that allows us to determine the aerotolerance of a microbe? Fluid Thioglycollate Media (FTM)
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