BIO 214-MRSA Case Study modified (1)

docx

School

Marian University *

*We aren’t endorsed by this school

Course

214

Subject

Biology

Date

Apr 3, 2024

Type

docx

Pages

Uploaded by MinisterTitanium13065

Microbiology Bio 214 Case Study MRSA Case Study Part I – Measuring Resistance Katelyn was excited to start her summer job in her microbiology professor’s research laboratory. She had enjoyed Dr. Johnson’s class, and when she saw the flyer recruiting undergraduate lab assistants for the summer, she had jumped at the opportunity. She was looking forward to making new discoveries in the lab. On her first day, she was supposed to meet with Dr. Johnson to talk about what she would be doing. She knew the lab focused on antibiotic resistance in Staphylococcus aureus especially MRSA (methicillin-resistant S. aureus). She still remembered the scare her family had last year when her little brother, Jimmy, got so sick. He had been playing in the neighborhood playground and cut his lip when he fell off the jungle gym. Of course, he always had cuts and scrapes—he was a five-year-old boy! This time though his lip swelled up and he developed a fever. When her mother took him to the doctor, the pediatrician said the cut was infected and had prescribed cephalothin, an antibiotic related to penicillin, and recommended flushing the cut regularly to help clear up the infection. Two days later, Jimmy was in the hospital with a fever of 103°F, coughing up blood and having trouble breathing. The emergency room doctors told the family that Jimmy had developed pneumonia. They started him on IV antibiotics, including ceftriaxone and nafcillin, both also relatives of penicillin. It was lucky for Jimmy that one of the doctors decided to check for MRSA, because that is what it was! MRSA is resistant to most of the penicillin derivatives. Most cases of MRSA are hospital- acquired from patients who are already susceptible to infection, but the ER doctor explained that community-acquired MRSA was becoming more common. The doctor then switched the treatment to vancomycin, a completely different kind of antibiotic, and Jimmy got better quickly after that. Katelyn had dropped Jimmy off at swimming lessons just before coming to work at the lab. As she waited in the hallway for Dr. Johnson, she hoped that she would be at least a small part of helping other people like Jimmy deal with these scary resistant microbes. She was surprised when the professor burst out of the lab, almost running into her. “Hi Katelyn, I’m really sorry but I have to run to a meeting right now—they sprung it on me last minute. There are bunches of plates in the incubator right now that need their zones of inhibition measured. I’ll be back in a few hours,” Dr. Johnson said as he rushed down the hallway with a stack of folders. Katelyn dug out her old lab notebook to look up what she was supposed to do. She found the lab where she and her fellow students had examined the antimicrobial properties of antibiotics using the Kirby-Bauer disk diffusion technique. Looking at

Microbiology Bio 214 Case Study the plates Dr. Johnson had told her about, she saw they had all been “lawned,” or completely coated with microbes to make a thick hazy layer over the agar surface. She could also see paper disks with letters on them, and some of the disks had clear zones around them where the microbe had been inhibited (Fig. 1). Her notebook explained how to measure the zone of inhibition around the disks (Fig. 2).

Microbiology Bio 214 Case Study Plate 1. Plate 2. Plate 3. S. aureus PE CE ME VA S. aureus PE CE ME VA S. aureus PE CE ME VA PE CE ME MRSA VA PE CE ME MRSA VA PE CE ME MRSA VA

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Microbiology Bio 214 Case Study Exercise 1 Measure the zones of inhibition for each antibiotic on the plates shown in Figure 1 and note the measurements in the spaces in Table 1 below. (Note: Th e Kirby-Bauer method is standardized so that no zone of inhibition is scored as a 0, and all others include the disk as part of the zone.) Key: PE = penicillin, ME = methicillin, CE = cephalothin, and VA = vancomycin Plate S. aureus MRSA 1 PE 25 0 ME 10 0 CE 21 0 VA 15 21 2 PE 24 0 ME 18 0 CE 23 0 VA 18 18 3 PE 28 0 ME 12.5 0 CE 30 0

Microbiology Bio 214 Case Study Plate S. aureus MRSA VA 10 13 An average, or mean (x), is a measure of central tendency in the data, or what value occurs in the middle of the data set. The mean is calculated by adding up all the values for a given set of data, then dividing by the sample size Average x = ∑ i = 0 n Xi ❑ Exercise 2 In Table 2 below calculate and record the averages and standard errors for each antibiotic in S. aureus and MRSA. S. aureus MRSA Average Average PE 24.6667 0 ME 13 0 CE 24 0 VA 14 16.33333 Exercise 3 Antibiotic MSSA MRSA PE Mean 24.6667mm Mean 0mm ME Mean 13mm Mean 0mm CE Mean 24mm Mean 0mm VA Mean 14mm Mean 16.33333mm Now, redraw Tables 1 and 2 into a single, more organized table. Be sure to label the table appropriately.

Microbiology Bio 214 Case Study Exercise 4 Graph the results from Table 2. Be sure to label the figure and the axes correctly. Graph the results here.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Microbiology Bio 214 Case Study Questions 1. What do you think the experimental question is? The question of the experiment is to analyze the vitro and sensitivity of different strains of bacteria with the use of different antibiotics . 2. What hypotheses can you come up with to answer the experimental question? The hypothesis of the experiment is bacterial strains for not experiencing sensitivity of different antibiotics. 3. If your hypothesis is correct, what would the plates look like (i.e., what predictions would you make for each hypothesis)? plates with antibiotics disc would have bacteria development 4. Data is perfect for the answer to answer 1 as the sensitivity of the strains 5. Is the experiment you just collected data for an appropriate test of the experimental question you came up with in your answer to Question 1? the antibiotic for S. Aureus would be most effective with PE for MRSA was VA 6. Which antibiotics where most effective against S. aureus? Which antibiotics where most effective against MRSA? all antibiotics used are effective in different quantities against S. aureus 7. When comparing the antibiotics effective against both, were there differences in effectiveness? Halo diameter has an effect on the colony with different antibiotics 8. What other questions do the data shown in Figure 1 make you think of? I can not think of any other questions I would have based on this *Modified from Antibiotic Resistance: Can We Ever Win?, case study by Maureen Leonard, The National Center for Case Study Teaching in Science, University at Buffalo, State University of New York.

Related Documents

LAB 04 EXERCISE 01.pdf

LAB 03 EXERCISE 08.pdf

Calculating Correlation Coefficients and Creating Lines of Best Fit.docx

virtual lab week 3.docx

BIOL201_EXAM1_2.png

BIOL201_EXAM1_5.png

Human-Microbe Interactions.docx

Quiz Gases in the Cockpit.docx

Sample Questions.doc

PDF NSG6435 Week 3 Case Study Roberts. S.pdf

2016.Exam2.KEY.pdf

Vital Signs Report.docx

Recommended textbooks for you

3-2-1 Code It

Biology

ISBN:9781337660549

Author:GREEN

Publisher:Cengage

Principles Of Pharmacology Med Assist

Biology

ISBN:9781337512442

Author:RICE

Publisher:Cengage

Microbiology for Surgical Technologists (MindTap ...

Biology

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Cengage Learning

Biomedical Instrumentation Systems

Chemistry

ISBN:9781133478294

Author:Chatterjee

Publisher:Cengage

Surgical Tech For Surgical Tech Pos Care

Health & Nutrition

ISBN:9781337648868

Author:Association

Publisher:Cengage

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:9781305143531

Author:CURREN

Publisher:Cengage

SEE MORE TEXTBOOKS

Recommended textbooks for you

3-2-1 Code It
Biology
ISBN:9781337660549
Author:GREEN
Publisher:Cengage
Principles Of Pharmacology Med Assist
Biology
ISBN:9781337512442
Author:RICE
Publisher:Cengage
Microbiology for Surgical Technologists (MindTap ...
Biology
ISBN:9781111306663
Author:Margaret Rodriguez, Paul Price
Publisher:Cengage Learning
Biomedical Instrumentation Systems
Chemistry
ISBN:9781133478294
Author:Chatterjee
Publisher:Cengage
Surgical Tech For Surgical Tech Pos Care
Health & Nutrition
ISBN:9781337648868
Author:Association
Publisher:Cengage
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:9781305143531
Author:CURREN
Publisher:Cengage