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Biology

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Apr 3, 2024

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Information Flow Lecture 1: The flow of biological information starts with the genetic code. PRE-CLASS WORK: READ: Ch. 3.6 Nucleic acids store, transmit, and help express hereditary information. 1. Complete the sketch of the DNA nucleotide below by labeling the following: Deoxyribose sugar Nitrogenous base Phosphate group Number each of the carbons on the sugar (1′, 2′, 3′, 4′, 5′) The free -OH group on the sugar The location on the sugar that makes deoxyribose different from ribose. 2. Nitrogenous bases: The purine bases are ______________________ and _______________ The pyrimidine bases are ________________ and ________________. In a molecule of DNA, a nucleotide with an adenine (A) base will always pair with a nucleotide with a ____________________ base. A nucleotide with a guanine (G) base will always pair with a nucleotide with a _____________ base. READ: Ch. 13.1 DNA is the genetic material. 3. Frederick Griffith’s classic experiment: What was Griffith’s central research goal? What was he studying at the time? Which of the four experimental results was unexpected? Explain. What hypothesis would explain the unexpected result? What did Griffith call this phenomenon? 1
4. DNA structure: 1. Using the last nucleotide in the chain below, label the three components of a DNA nucleotide: nitrogenous base, deoxyribose sugar, and phosphate group. 2. Circle a phosphodiester bond. (Your textbook calls this a sugar-phosphate covalent bond) 3. Label the 5’ and 3’ ends of the chain. 4. What chemical group is attached to the 5’ carbon of the deoxyribose sugar? ______________________ 5. What chemical group is attached to the 3’ carbon of the deoxyribose sugar? ______________________ 6. What makes up the backbone of a DNA molecule? ________________________ 7. The two sides of a DNA molecule are held together by ________________ bonds. 2. DNA has an antiparallel structure, with the ends of the two strands identified as 5’ and 3’. Make a brief simplified sketch below that demonstrates the antiparallel structure of DNA, using 5’ and 3. (Figure 13.8 has a nice review of the different ways that a DNA molecule may be drawn from detailed to simplified.) END OF PRE-CLASS 2
C ricket evolution: Identify mechanism of information flow at each level. Flatwing male normal normal Gene Mutant gene involved in hormone signaling RNA/Protein Cellular Action Phenotype Behavior Environment Identify mechanism of information flow at each level. 3
Was Griffith’s “transforming principle” protein or DNA? Frederick Griffith (1928) “ transforming principle” Alfred Hershey and Martha Chase (1952) DNA from a virus enters the cell, not protein. Oswald Avery Maclyn McCarty (b. South Bend, IN 1911) Colin MacLeod DNA is responsible for bacterial transformation. (1944) The structure of DNA is essential for understanding its function in information flow. James Watson and Francis Crick (1953) Maurice Wilkins Raymond Gosling Rosalind Franklin Photo 51 4
Fidelity: the driving force of why information flow works. The “telephone game” - no fidelity checks ANALYSIS: Identify at least 3 ways in which the structure of DNA provides stability and fidelity of the genetic code. How does the complementarity of DNA help ensure fidelity? 5
"It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material." - Watson and Crick, Nature, 1953 T A G C phosphate group deoxyribose sugar nitrogenous base 5’ end 3’ end phosphodiester bond nucleotide REPLICATE the DNA: DRAW the complementary strand including the correct number of hydrogen bonds, label the 5’ and 3’ ends 6
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