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Information Flow Lecture 1: The flow of biological information starts with the genetic code. PRE-CLASS WORK: READ: Ch. 3.6 Nucleic acids store, transmit, and help express hereditary information. 1.
Complete the sketch of the DNA nucleotide below by labeling
the following:
●
Deoxyribose sugar
●
Nitrogenous base
●
Phosphate group
●
Number each of the carbons on the sugar (1′, 2′, 3′, 4′, 5′)
●
The free -OH group on the sugar
●
The location on the sugar that makes deoxyribose different from
ribose.
2.
Nitrogenous bases:
●
The purine bases are ______________________ and _______________
●
The pyrimidine bases are ________________ and ________________.
●
In a molecule of DNA, a nucleotide with an adenine (A) base will always pair with a nucleotide with a
____________________ base.
●
A nucleotide with a guanine (G) base will always pair with a nucleotide with a _____________ base.
READ: Ch. 13.1 DNA is the genetic material. 3.
Frederick Griffith’s classic experiment:
●
What was Griffith’s central research goal? What was he studying at the time?
●
Which of the four experimental results was unexpected? Explain.
●
What hypothesis would explain the unexpected result? What did Griffith call this phenomenon?
1
4. DNA structure: ● 1. Using the last nucleotide in the chain below, label the three components of a DNA nucleotide: nitrogenous base, deoxyribose sugar, and phosphate group. ● 2. Circle a phosphodiester bond. (Your textbook calls this a sugar-phosphate covalent bond) ● 3. Label the 5’ and 3’ ends of the chain. ● 4. What chemical group is attached to the 5’ carbon of the deoxyribose sugar? ______________________ ● 5. What chemical group is attached to the 3’ carbon of the deoxyribose sugar? ______________________ ● 6. What makes up the backbone of a DNA molecule? ________________________ ● 7. The two sides of a DNA molecule are held together by ________________ bonds. 2. DNA has an antiparallel structure, with the ends of the two strands identified as 5’ and 3’. Make a brief simplified sketch below that demonstrates the antiparallel structure of DNA, using 5’ and 3. (Figure 13.8 has a nice review of the different ways that a DNA molecule may be drawn from detailed to simplified.) END OF PRE-CLASS 2
C
ricket evolution: Identify mechanism
of information flow at each level. Flatwing male
normal
normal
Gene
Mutant gene involved in hormone signaling
RNA/Protein
Cellular Action
Phenotype
Behavior
Environment
Identify mechanism
of information flow at each level. 3
Was Griffith’s “transforming principle” protein or DNA? Frederick Griffith (1928) “ transforming principle”
Alfred Hershey and Martha Chase (1952)
DNA from a virus enters the cell, not protein. Oswald Avery
Maclyn McCarty (b. South Bend, IN 1911)
Colin MacLeod
DNA is responsible for bacterial transformation. (1944)
The structure of DNA is essential for understanding its function in information flow. James Watson and Francis Crick (1953)
Maurice Wilkins
Raymond Gosling
Rosalind Franklin
Photo 51
4
Fidelity: the driving force of why information flow works. The “telephone game” - no fidelity checks ANALYSIS: Identify at least 3 ways in which the structure of DNA provides stability and fidelity of the genetic code. How does the complementarity of DNA help ensure fidelity? 5
"It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material."
-
Watson and Crick, Nature, 1953
T
A
G
C
phosphate group deoxyribose sugar nitrogenous base
5’ end
3’ end
phosphodiester bond
nucleotide
REPLICATE the DNA:
DRAW the complementary strand including the correct number of hydrogen bonds, label the 5’ and 3’ ends
6
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Related Questions
Identify the type of base and sugar group present in each nucleotide or nucleoside.
HO.
H3C.
CH₂
OH
-²03POCH2
OH
'N
N
ΝΗ
NH₂
Molecule 1
O pyrimidine, deoxyribose
O pyrimidine, ribose
purine, ribose
O purine, deoxyribose
Molecule 2
pyrimidine, ribose
O pyrimidine, deoxyribose
purine, ribose
O purine, deoxyribose
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Please help with this practice problem!
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name and identify
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b. What is the difference between the 3' and the 5' ends of a nucleotide chain?
C. Do the chains run the same way?
d. How are the chains connected?
e. Which bases bond to each other?
f. What kinds of bonds hold the chain together?
3. What are the main differences between RNA and DNA?
4. Distinguish between the structure of pyrimidines and purines. Explain why adenine
bonds only to thymine.
5. Name the five nitrogenous bases in the table below, and put an X in the correct column
for each base. Then indicate if the base if found in DNA (D), RNA (R), or both (B)
hp
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H2N
1.) Look carefully at this nucleotide:
N:
N-
Но-Р-О
OH
a.) Number the carbons in the sugar group. (Remember the "prime" symbols.)
b.) Is this a purine or a pyrimidine? How do you know?
c.) Would this nucleotide be used for DNA or RNA? How do you know? (Be specific.)
d.) Is this nucleotide ready to be used for DNA replication or RNA transcription? Why/why not?
e.) If this nucleotide were incorporated into a growing DNA or RNA strand, where would the
next added nucleotide be attached to this one?
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a. Write the structural formula of GAC, a portion of DNA. Write
the complementary strand adjacent to it so that the
complementary bases are side by side. Connect the
appropriate base pairs.
b. Sticking to the convention of writing the nucleotide sequence
in the 5'-3' direction, what is the nucleotide sequence of the
DNA strand complementary to ATGCACCATGCT?
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E. PROTEIN PRIMARY STRUCTURE ELUCIDATION.
1. Determine the primary structure of the protein described below. Write the final sequence
using the corresponding three-letter code for each amino acid.
Example:
M-F-Y-R should be written as Met-Phe-Tyr-Arg
Treatment with cyanogen bromide and sequencing yields the following peptide fragments:
o D-M
o R-A-Y-G-N
o L-F-M
Chymotrypsin digestion and sequencing yields the following peptide fragments:
o G-N
D-M-L-F
o M-R-A-Y
o o
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ture.com/courses/7611/assignments/170598?module_itemid%3D695428
TR E Edulastic: Formativ. A Desmos | Testing
O YouTube I Quiz | ReadTheory
Quill.org - In
О в, С, А, D
D, B, A, C
C, B, D, A
How many nucleotides are represented in the diagram? *
2 points
O 6
12
18
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Question Guide:
1. Name and write the structures of the purine and pyrimidine bases present in DNA.
Purine
Рyrimidine
Structures:
Purine
Pyrimidine
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Draw atleast 3 structure of nucleotide containing 2 pyrimidine bases and I purine base.
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Tick the correct statements:
Remember: Tautomers are structural isomers that differ from each other based on the position of the proton(s) and their double bonds.
( ) The nitrogenous bases of nucleic acids, which contain heterocyclic and analogous nuclei, can adopt different tautomeric forms involving multiple H+ that are exchangeable depending on the medium. In DNA, spontaneous formation of smaller tautomers appears to contribute to mutagenic errors during DNA replication, while in RNA, they seem to be related to increased structural and functional diversity of enzymes and RNA aptamers (research this and confirm if it is false or real)
( ) in relation to the figure, anomer 1 has beta stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond
( ) in relation to the figure, anomer 1 has alpha stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond
( ) In general, in naturally occurring nucleosides,…
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Name the nucleotide.
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Pls help
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Go to Figure 5.23 a and, for the top three nucleotides, number all the carbons in the sugars, circle the nitrogenous bases, and star the phosphates.
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9L.12
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N.
NH
2. One of the key pieces of information that Watson
and Crick used in determining the secondary
structure of DNA came from experiments done by E.
Chargaff, in which he studied the nucleotide
composition of DNA from many different species.
O=P-OCH,
N.
`NH,
HN
он
O= P- OCH,
NH,
Chargaff noted that the molar quantity of A_was
always approximately equal to the molar quantity of
T. and the molar quantity of C was always
approximately equal to the molar quantity of G. How
were Chargaff's results explained by the
structural model of DNA proposed by Watson
and Crick?
N
OH
N.
O= P-OCH,
OH
OH
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Do (9)(a)
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(a) Draw diagrams to show how the four synthetic oligonucleotides below could base-pair to form a
stable model Holliday junction.
W 5' GATCGCATTGTAGCCGTAGGTCCACTGTAA 3’
X 5' GTCCCATACGTAGCCGTAGGACATGTACCG 3'
Y 5' CGGTACATGTCCTACGGCTACAATGCGATC 3'
Z 5' TTACAGTGGACCTACGGCTACGTATGGGAC 3'
I and 21
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These multiple-choice questions are all related to the Basic Biological Principles (DNA) | Introduction to Biomolecules. Choose the best answer on the given choices.
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Give explanation of correct option and explanation of incorrect options.
Hand written solutions are strictly prohibited.
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Compare and contrast the structure of DNA and RNA. Be sure to
describe each of the three components of a nucleotide for both DNA and RNA along with the types of bonds formed between the components. In addition, explain: how the nucleotides link together to form each molecule, why the prime ends are labeled 5’ and 3’, what antiparallel is, what phospodiester linkages are and what complementary base pairing is.
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Help me please...
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Which of the following is true of the molecule below?
nucleotide 5'
3′
H₂C
H₂C
TNHNA
O. HN
CNH…NG
NH O
NH O
HNU
NH O
CNHNG
OHN
nucleoside
CH₂
CH₂
a purine pairs with a pyrimidine base
the bases are held together by covalent bonds
a nucleoside is made of the same components as a nucleotide
The bases make up the backbone on the sides of the molecule
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Describe the functions of the following proteins during DNA breaks and repair:
(i)
Ku70
(ii)
Uracil DNA glycosylase
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(Optional) Describe the structure of DNA, discussing the DNA helix and the base pairs of
DNA, the overall structure of a chromosome, the structure of a gene, an mRNA, a
ribosome, and a protein. What are the relative sizes of each of these?
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A genetics student was asked to draw the chemical structureof an adenine- and thymine-containing dinucleotide derivedfrom DNA. His answer is shown below. The student made morethan six major errors. One of them is circled, numbered 1, andexplained. Find five others. Circle them, number them 2 to 6, andbriefly explain each by following the attached given.
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choices:purine or pyrimidine
ribose or deoxyribose
1-9?
hydrogen or phosphodiester
1-9?
an -OH or no -OH
1-9?
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Identify the correct name or abbreviation for the given
nucleoside or nucleotide.
guanosine
ADP
dADP
dGDP
GDP
Identify the correct name or abbreviation for the given
nucleoside or nucleotide.
GDP
dADP
ADP
O deoxyadenosine
dGDP
OMP
O
||
-O-P-O-P-O.
fot
O™
tt
-O-P-O-
O™
ܘ ܐ ܘ ܐ
OIPIO
N
OH OH
OH
N
H₂N
N
ΝΗ
N
NH₂
arrow_forward
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Related Questions
- Identify the type of base and sugar group present in each nucleotide or nucleoside. HO. H3C. CH₂ OH -²03POCH2 OH 'N N ΝΗ NH₂ Molecule 1 O pyrimidine, deoxyribose O pyrimidine, ribose purine, ribose O purine, deoxyribose Molecule 2 pyrimidine, ribose O pyrimidine, deoxyribose purine, ribose O purine, deoxyribosearrow_forwardPlease help with this practice problem!arrow_forwardname and identifyarrow_forward
- b. What is the difference between the 3' and the 5' ends of a nucleotide chain? C. Do the chains run the same way? d. How are the chains connected? e. Which bases bond to each other? f. What kinds of bonds hold the chain together? 3. What are the main differences between RNA and DNA? 4. Distinguish between the structure of pyrimidines and purines. Explain why adenine bonds only to thymine. 5. Name the five nitrogenous bases in the table below, and put an X in the correct column for each base. Then indicate if the base if found in DNA (D), RNA (R), or both (B) hparrow_forwardH2N 1.) Look carefully at this nucleotide: N: N- Но-Р-О OH a.) Number the carbons in the sugar group. (Remember the "prime" symbols.) b.) Is this a purine or a pyrimidine? How do you know? c.) Would this nucleotide be used for DNA or RNA? How do you know? (Be specific.) d.) Is this nucleotide ready to be used for DNA replication or RNA transcription? Why/why not? e.) If this nucleotide were incorporated into a growing DNA or RNA strand, where would the next added nucleotide be attached to this one?arrow_forwarda. Write the structural formula of GAC, a portion of DNA. Write the complementary strand adjacent to it so that the complementary bases are side by side. Connect the appropriate base pairs. b. Sticking to the convention of writing the nucleotide sequence in the 5'-3' direction, what is the nucleotide sequence of the DNA strand complementary to ATGCACCATGCT?arrow_forward
- E. PROTEIN PRIMARY STRUCTURE ELUCIDATION. 1. Determine the primary structure of the protein described below. Write the final sequence using the corresponding three-letter code for each amino acid. Example: M-F-Y-R should be written as Met-Phe-Tyr-Arg Treatment with cyanogen bromide and sequencing yields the following peptide fragments: o D-M o R-A-Y-G-N o L-F-M Chymotrypsin digestion and sequencing yields the following peptide fragments: o G-N D-M-L-F o M-R-A-Y o oarrow_forwardture.com/courses/7611/assignments/170598?module_itemid%3D695428 TR E Edulastic: Formativ. A Desmos | Testing O YouTube I Quiz | ReadTheory Quill.org - In О в, С, А, D D, B, A, C C, B, D, A How many nucleotides are represented in the diagram? * 2 points O 6 12 18arrow_forwardQuestion Guide: 1. Name and write the structures of the purine and pyrimidine bases present in DNA. Purine Рyrimidine Structures: Purine Pyrimidinearrow_forward
- Draw atleast 3 structure of nucleotide containing 2 pyrimidine bases and I purine base.arrow_forwardTick the correct statements: Remember: Tautomers are structural isomers that differ from each other based on the position of the proton(s) and their double bonds. ( ) The nitrogenous bases of nucleic acids, which contain heterocyclic and analogous nuclei, can adopt different tautomeric forms involving multiple H+ that are exchangeable depending on the medium. In DNA, spontaneous formation of smaller tautomers appears to contribute to mutagenic errors during DNA replication, while in RNA, they seem to be related to increased structural and functional diversity of enzymes and RNA aptamers (research this and confirm if it is false or real) ( ) in relation to the figure, anomer 1 has beta stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) in relation to the figure, anomer 1 has alpha stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) In general, in naturally occurring nucleosides,…arrow_forwardName the nucleotide.arrow_forward
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Human Heredity: Principles and Issues (MindTap Co...
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ISBN:9781305251052
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