DNA Gel electrophoresis & PCR Worksheet-Lydia Specht

In details, compare the life cycles of influenza virus and measles virus (4-5 points of contrasting). Thank you!

Discuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods: Restriction Fragment Length Polymorphism

please tell me how  bacteriophage and animal viruses multiply. please add both lytic and lysogenic cycles for bacteriophage, all steps, and the outcomes of both.  For animal viruses, please include all steps and briefly explain the differences between their nucleic acids. i am having a hard trouble understanding this information if you could please explain in way where a child would understand it that would be amazing thank you

2. Viruses a. Are viruses living organisms? Why? i. Give the properties of a virus that would differentiate it with bacteria. ii. Give the types of infection that a virus can cause. iii. Provide the definition of the following: 1. provirus 2. viroids 3. virions References: Add your reference where you got your citations for your answers in this laboratory activity.

just answer question ---  dont need explanation

This is question is from my micro biology class. 1. OUTLINE the steps that lead to the production of proteins from the genome of a double stranded RNA virus. List any PROCESSES involved and any ENZYMES.

JUST PROVIDE A BRIEF ANSWER. PLEASE HELPE OUT, I'M BEGGING YOU PLEASE. THANK YOU SO MUCH IN ADVANCE.

OF rasc PDF Teas fill Which of the following is NOT a step in the replication of a retrovirus? A diagram illustrating the process is provided below. PDF UNOFFIC TRANSCR 59°F Mostly cloudy Virus Viral RNA Reverse transcriptase DDDDDD ↓Viral DNA Provirus mRNA mm Proteins mn Viral RNA Protease Plasma membrane Host DNA New virus O The single-stranded DNA forms a double-stranded DNA called a provirus, which is incorporated into the host cell DNA. O After a retrovirus attaches to the host cell, it injects its viral DNA and uses the host cell's machinery and materials to replicate. O After a retrovirus injects its viral RNA into a host cell, it forms a DNA strand by reverse transcription. O When the host cell replicates, a provirus produces the viral RNA needed to produce more virus particles. P O Search hun recc PDF maste omiss

Related top medical coding, what type of code(s) is/are specific to the disease? ICD 9 ICD 10 Procedure codes CPT codes

Viruses: a. Describe the structure and composition of viruses. What are three reasons that they are different from cellular organisms? b. Describe what a lysogenic bacteriophage is and how it is different from a lytic bacteriophage. c. Describe what is similar and different about the lifecycle of a non-enveloped DNA animal virus compared to an enveloped animal retrovirus.

Give Detailed Solution...(no need Handwritten)

After giving brief information about the viruses, discuss briefly what is meant by their virulent effects on their hosts and in which two ways this effect occurs. Comment and illustrate the use of viruses within the scope of Recombinant DNA Technology.

Answer the following  1.Are viruses living or non-living organisms? 2. What is the difference between HIV and AIDS? 3. HIV is classified as a retrovirus. What are retroviruses? 4. In your own words, provide a short summary of the HIV replication cycle. 5. Why is reverse transcriptase an important enzyme in the replication cycle of HIV? 6. In the chapter about enzymes, we have discussed how drugs are basically inhibitors. There are currently a multitude of Antiretroviral therapy (ART or ARV) options available to people living with HIV (PLHIV) so that the viral replication is suppressed and does not progress to AIDS. What do you think these drugs target? Discuss briefly. 7. SARS-CoV-2, the virus that causes CoVid-19, belongs to the Coronaviridae family. Viruses under this family have a genome made of single-stranded positive-sense RNA. Is this similar to the viral genome of HIV? If so, do you think that – in theory – they would have similar replication cycles? 8. In your own opinion, do…

Describe the process of replication of retroviruses. (You can use HIV as an example). Details are required, including the names of key enzymes involved. More than 10 sentences are required to get full credit.

please tell me how  bacteriophage and animal viruses multiply. please add both lytic and lysogenic cycles for bacteriophage, all steps, and the outcomes of both.  For animal viruses, please include all steps and briefly explain the differences between their nucleic acids. i am having a hard trouble understanding this information if you could please explain in way where a child would understand it that would be amazing

explain what the red box (figure 1) is showing. the figure 1 description is confusing please help me understand

Give detailed Solution with explanation needed (don't copy the answer other site

Identify the numbered steps of this viral life cycle/replication cycle depicted here.

8) Label each method of mixing genetic material as belonging to dsDNA viruses, retroviruses, SARS CoV-2, IAV, or not a method of mixing genetic material. homologous recombination template switch recombination template switch recombination Segmented genomes Having RDRP with proofreading activity

Discuss the principles behind the use of the following techniques in the diagnosis of viral infections, providing examples of viruses diagnosed using these methods 5..1 ELISA 5.2 Restriction Fragment Length Polymorphism 5.3 Polymerase chain reaction

Please provide a general definition of a virus. Viruses can cause other issues, known as cytopathic effects. Provide 3 effects and discuss why that effect can be very damaging to the host.

Discuss the advantages and disadvantages of the use of the following viruses as vectors Retroviruses Adenoviruses Adeno-associated viruses

Why are animal viruses grown in eggs and not in culture media (nutrient broth only)? (not assigned; will review as a class)

A chronic IV drug user is admitted to the hospital after a suspected overdose. During recovery the patient’s bilirubin was measured at 17.5 g/dL. What infectious disease testing would you perform? What diagnostic testing methodology might be utilized? Speculate what infectious disease the patient has. What treatment options are available? Infants and neonates are at high risk for contracting numerous different viruses. Name three common viruses that infect this population. Describe testing methodology used to identify the viruses, and treatment options for each

NOTE: explain in a paragraph form How does PCR help in the identification of the virus from patients?

The procedure involved doing Plaque assays of E coli C by performing serial dilutions then using TSA plates where the host for Ecoli C and each dilution was put onto a plate to observe the plaques... please help with this question, thank you.

The complete coronavirus genome sequence from the first known patient infected with SARS-CoV-2 was published on January 10th 2020, just a short time after the patient had been hospitalized in China with respiratory symptoms. They did this so quickly using nanopore sequencing. Scientists compared the genome sequence from the new coronavirus (SARS-CoV-2) with the genome sequences from several other coronaviruses and they did identify a sequence unique to the SARS-CoV-2 strain. This unique sequence was in the N gene of the viral genome. What protein is made from the N gene of the virus?

You are researching how the virus that you are studying spreads through a host. Which of the following assays would be the most advanced to evaluate viral spread in real-time? euthanization of SCID mice followed by dissection and flow cytometry euthanization of mice infected with engineered viruses that express reporter proteins and staining for protein expression euthanization followed by dissection and assaying for the virus in tissues MRI imaging of mice expressing viral constructs that express bioluminescence

Question:- a. Describe the characteristics used to classify viruses. b. List five specific properties of viruses that distinguish them from bacteria. c. Discuss differences between proviruses, viroids, and prions, and the diseases they cause.

Hi, can you kindly make 3 concepts that is related to the research topic which is "Compromising own health care in hospitals due to covid-19 virus.   Here's the format below:   Concept 1: (Title that is related to the research topic as mentioned above") Definition: (meaning) Description: (Functions, importance, features, effects, disadvantages and advantages)   Concept 2 and Concept 3 are same to concept 1's format but each have different title.   Kindly put the links of sources too

Please help me answer this reviewer. Write the word TRUE if the statement is correct and if not, underline the word or statement that makes the sentence incorrect and write the correct answer on the space provided. 1. CRISPR refers to the repeated sequences located in the viral DNA.  answer: 2. In CRISPR, the guide RNA is designed to find and bind to a specific sequence in the DNA.  answer: 3. PCR makes it possible to obtain multiple copies of DNA fragments from an extract.  answer: 4. DNA is positively charged.  answer: Thank you very much for your help

I need the answer as soon as possible

Question: Gene therapy: a) what disease is it used for? b) what genetic defect causes the disease? c) what and how viral vector is used AND/OR CRISPR-Cas is used? d) what is in the horizen re. development of new virus-based gene delivery vesicle?

Please help me with this question. More than one answer may be correct. THe graph relating to the information is included below. The above graph is looking at the relative entry of SARS-CoV2 isolated spike proteins into human cells in culture at two times points: before introduction of the virus to the cell culture (0 minute) and 5 minutes after introduction of the virus to the cell culture. CHC stands for "clathrin heavy chain" and siRNA stands for small interfering RNA, which is often used in experiments as an easy method to stop expression of a certain gene. So in this case, the CHC siRNA would stop cellular production of CHC. The control siRNA introduces a small RNA fragment that does not match any gene as a negative control. From the above figure, you can conclude what about the process that allows the SARS CoV-2 virus entry into the cell? Options: The spike protein is not involved in cell entry, contrary to popular belief This is evidence for clathrin-mediated endocytosis as the…

Please help me with this question. More than one answer may be correct. The graph relating to the information is included below. The above graph is looking at the relative entry of SARS-CoV2 isolated spike proteins into human cells in culture at two times points: before introduction of the virus to the cell culture (0 minute) and 5 minutes after introduction of the virus to the cell culture. CHC stands for "clathrin heavy chain" and siRNA stands for small interfering RNA, which is often used in experiments as an easy method to stop expression of a certain gene. So in this case, the CHC siRNA would stop cellular production of CHC. The control siRNA introduces a small RNA fragment that does not match any gene as a negative control. Imagine that the experiment was then allowed to run for 500 minutes in addition to five minutes? How would the relative uptake of the spike protein into the control siRNA cells compare after 500 min to the 5 minute treatment? Options: 500 min time point would…