Microbiology and fermentation (lab exam 1) Flashcards _ Quizlet

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3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-ﬂash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 1/6 Microbiology and fermentation (lab exam 1) Leave the first rating Students also viewed Terms in this set (132) Others also viewed these textbooks Search for a textbook or question Hole's Human Anatomy and Physiology 13th Edition • ISBN: 9780073378275 (15 more) David N. Shier, Jackie L. Butler, Ricki Lewis 1,458 solutions Human Microbiome 61 terms ceabennett01 Preview Bio Test #2 74 terms MayoIzYum Preview BIOL 1720 Exam 3 35 terms amypritchard6712 Preview Microbiolog 9 terms Eric_Tand What is the maximum number of excused absences allowed? one week worth of class How many lab sessions can one miss before they are withdrawn? two consecutive lab sessions or a total of three Is lab mandatory Yes Can a student make up a lab if they miss their normal lab session yes as long as they attend a lab that is conducting the same lab work Is taping of lecture and lab permitted? NO If a culture is spilled should the student attempt to clean it up themselves? NO In the case of a culture spill, alert the instructor immediately!! The procedure for decontaminating a spill and the must be strictly followed!!! If misplaced items are found in your storage drawer, such as another students slide, where should you place them? In the "orphaned slides, tubes and plates" area Should one turn off the water faucets with bare clean hands after washing? No, always use a paper towel to turn off faucets! When cleaning up a spill how should you wipe down the bench? always use a rotating motion of the hand to bring the paper towels toward the middle of the spill area .... Never use the "windshield wiper" method!! The working distance on your microscope ... decreases with increasing magnification Microbiology and fermentation (lab exam 1) Study

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-ﬂash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 2/6 Why should you use only fine focus knob while viewing a specimen with the 100x objective? Because working distance is minimal and if the coarse adjustment is used you are likely to shatter the slide or the objective lens In case of accidental spill you would... remain at your work station until instructor comes to your assistance Why is a wet film of disinfectant left on the lab bench to air dry? If you wipe the lab bench completely dry there is no disinfectant left on the bench top which leaves the bench top open for contamination from the paper towel you're wiping with, aerosols and many other things To prevent aerosols from forming, wires that from loops and needles are flamed... from handle connection to tip (loop) Why are loops flame sterilized from handle to tip (loop) to prevent aerosols from forming and being spread When do you sterilize the inoculating loop? Before obtaining a sample and after transferring the bacteria to another medium or slide At a minimum how many times are you required to wash your hands for this lab? 2 (one at the start and one at the end) The iris diaphram of the microscope controls the amount of light passing through the condenser Which microscope is used to visualize viruses? Electron The total magnification of the specimen is 200x. If the ocular lens used on the microscope was 20x, then which objective lens was used? 10x Which staining technique results in an unstained cell against a dark or colored background? Negative staining The counterstain of the Gram stain is Safranin Iodine of the Gram stain serves as the mordant The basic categories of colony morphology are shape, margin, texture, elevation, pigment production A device used for counting colonies is called a colony counter a word that means in the absence of oxygen anaerobic The process for removing oxygen in the anaerobic jar works how? a packet containing chemiclas that react oxygen gas with hydrogen gas producing water a device used for producing an oxygen free environment is called an anaerobic jar How will you know when the anaerobic jar chamber is void of oxygen? the methylene blue indicator strip will appear clear or white Is the counter stain in an acid fast stain basic or acidic? basic Acid fast stains aid in the diagnosis of which diseases? leprosy, tuberculosis, and Nocardiosis The Gram and acid-fast stains are referred to as differential stains because They color various structures in bacterial cells contrasting colors The Schaeffer-Fulton staining procedure is used to stain cells with Endospores Microbiology and fermentation (lab exam 1)

3/25/24, 11:13 AM Microbiology and fermentation (lab exam 1) Flashcards | Quizlet https://quizlet.com/36381054/microbiology-and-fermentation-lab-exam-1-ﬂash-cards/?funnelUUID=34fe10fe-6e62-4061-9971-3ccf01de499e 3/6 Endospores exist in a cryptobiotic state means what They have no measurable metabolic activity When making a hanging drop preparation, a drop of water and a drop of bacteria culture are added to _______ a cover slip Colony morphology is affected by what? Genetics of organism, Incubation times, Nutrient availability, Temperature How long does the reaction take to produce an anaerobic jar vessel with little to no oxygen present? Up to one hour List factors that affect pigment production by colonies pH, incubation temperature define a colony a colony refers to individual organisms of the same species living closely together, usually for mutual benefit, A colony contains only ONE single type of microbe what are the basic shapes described in colony morphology round, irregular, and punctiform ( tiny, pinpoint) In colony morphology the margin (edge) may be described how entire- smooth with no irregularities undulate- wavy lobate- lobed filamentous or rhizoid- branched like roots Elevations in colony morphology include flat, raised, convex, pulvinate (very convex), umbonate (raised in center) pigment production is combined with optical properties such as opaque, translucent, shiny or dull B. Subtillis was what color and what did the margin appear like after incubation? white in color with filamentous margin What color was M. Lutea mustard yellow what color was S. Marcescens red when we made mixed plates which three bacterium were on Mix A B. Subtillis, S. Marcescens, S. Aureus When we made mixed plates which three were on Mix B M. Lutea, E. coli, S. Epidermidis What are consequences of staining or decolorizing for too long or not long enough? Can give false positives Gram positive cells look _____ because of a ______ ________ cell wall purple, thick peptidogylcan Gram neg. cells are colored_____ due to a ____ _____ cell wall sandwiched inbetween two membranes pink, thin peptidoglycan in Gram staining what is considered the mordant Iodine in gram staining what chemical is the decolorizer acetone and isopropanol Give steps of gram staining cells heat fix cells to slide, crystal violet, iodine, decolorizer, safranin, blot dry with bibulous paper The _____ stain is the most common used differential stain in bacteriology gram Microbiology and fermentation (lab exam 1)

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