BIO 101 Photsyn-Lab-Handout
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Exploring Photosynthesis and Plant
Pigments Darnell Lawrence
01/03/2024
Data Activity 1: Plant Pigments Overview Data Table 1: Chromatography of Plant Pigments Band # Distance from origin (mm) Band Color/ Identification 1 16 mm
Dark Green
2 17 mm
Green
3 23 mm
Light Green
4 25 mm
Lime green
Solvent
front 51 mm
Clear
1.
Many things can affect the evaporation of solvents during a chromatography experiment. To allow researchers to compare results across experiments under different conditions, scientists use an R
f
value, that is the ratio of the distance the pigment traveled to the distance the solvent traveled. This number remains constant across trials. Calculate the R
f
value for each pigment. Band one 16/51= 0.3 mm
Band two 17/51=0.3mm
Band three 23/51=0.4mm
Band four 25/51=.5mm
Activity 2: Leaf Disk Assay Overview 2.
Write a hypothesis that the Leaf Disk Assay is designed to test.
If leaf disks are exposed to light, they will be able to photosynthesize, while the leaf disks that are not exposed to light (kept in the dark) will not be able to photosynthesize.
Data Table 2: Leaf Disk Assay, Light Treatment Time
(minute
s) A
(0.24%
) B (0.24%
) Average
of A and B A
(0.12%
) B
(0.12%
) Average
of A and B 0 0
0
0
0
0
0
5 0
0
0
0
0
0
10 3
3
3
0
0
0
15 4
5
4.5
2
1
1.5
20 8
8
8
3
1
2
25 8
10
9
4
3
3.5
30 10
10
10
4
4
4
35 10
10
10
4
4
4
40 10
10
10
4
6
5
45 10 10
10
7
7
7
50 10
10
10
8
8
8
55 10
10
10
10
10
7
60 10
10
10
10
10
7.5
Data Table 3: Leaf Disk Assay, Dark Treatment Time
(minute
s) A
(0.24%
) B (0.24%
) Average
of A and B A
(0.12%
) B
(0.12%
) Average
of A and B 0 0
0
0
0
0
0
5 0
0
0
0
0
0
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DATA
Table 2
Table 3
Absorbance
Absorbance
Absorbance
Time
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unboiled
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Chloroplast
(min)
photosynthesis
light
dark
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143
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Unboiled
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5
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Dark
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10
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Boiled
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15
.]53
161
. 140
20
.148
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