Salinity Tolerance Lab 3

Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions               Solution          Volume of BSA     standard solution (μL)   Protein content(μg/mL) Absorbance value         At 595 nm                   1                 0                   0                 0                   2                 10                   1             0.022                   3                 30                   3             0.065                   4                 50                   5             0.106                   5                 70                   7             0.178                   6               100                 10             0.299                   7               120                 12             0.380         Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…

Water Properties Submit 4 7) 8. 10 1 of 10 B IU x X, 5 C 0/10000 Word Limit (d) The arrangement of the water molecules in ice causes the ice to float. Explain how ice floating on the surface of a body of water affects the water in a way that beneficial to the organisms in it. В I U x? X2 5 ¢ Ω 0/10000 Word Limit II !!

Question: Write a discussion and conclusion based on the graph

pH expresses the molar concentration of hydronium ions in an aqueous solution on a logarithmic scale. (Note that it is common to use the hydrogen ion, H*, to represent the hydronium ion, H3O+.) pH = -log[H3O+] [H3O+] = 10-PH As pH decreases, acidity increases. As pH increases, acidity decreases. • Acidic solution: pH 7 Part A - Calculating pH Submit [H₂0+] 1 Acidic pH 01 10¹ 102 103 104 105 106 107 108 109 10-10 10-11 10-12 10-13 10-14 stomach acid 3 wine lemon tomatoes juice vinegar, black soft drinks, coffee beer 6 Neutral urine milk 7 human blood pure water sea- water baking soda 10 11 12 13 14 milk of magnesia house- hold ammonia oven cleaner Basic household bleach Adapted from Biological Science by Freeman ©2008 Pearson Education, Inc. What is the pH of an aqueous solution with the hydronium ion concentration [H3O+] = 2 x 10-14 M? Make sure that your answer has the correct number of significant figures. For help determining the correct number of significant figures, see Hint 3. ►…

How is a mixture of ions separated using qualitative analysis? Group of answer choices Electrolysis of ions by variable voltage. Selective precipitation under acidic/basic conditions and electrolysis of ions by variable voltage. Selective precipitation under acidic/basic conditions. Selective precipitation under acidic/basic conditions and formation of complex ions. Selective precipitation under acidic conditions. Selective precipitation under acidic/basic conditions, formation of complex ions and electrolysis of ions by variable voltage. Formation of complex ions. Selective precipitation under basic conditions.

Report Sheet: Proteins (page 3) What kind of protein is casein? Name: POST-LABORATORY QUESTIONS: What is the isoelectric point of casein? What is acid in our sour milk? How is it produced? What type of chemical grouping is present in all proteins? How many of these chemical groupings must be present in a molecule to give a positive Biuret test? Give the principle involved and the chemical structure responsible for the positive Biuret Test? Can you distinguish proteins and other amino compounds by the Ninhydrin test? Explain. 30 | Biochemistry

Why is pH important for the buffer?   The pH is important to the stability of the gel.     The pH is important to the charge of the solution.     The pH is important to the charge and stability of many types of molecules, especially biomolecules like enzymes, proteins, RNA and DNA.     Submit Answer

Match the common laboratory scenarios to the correct buffer required using the guide below. Molecule pKa Value(s) Acetic acid 4.8 Carbonic acid 6.1, 10.2 Citric acid 3.1, 4.8, 5.4 HEPES 7.5 Phosphoric acid 2.1, 7.1, 12.3 TRIS 8.3                                                                                   Mammalian cells require a pH of about 7.4 for cell and tissue culture experiments.                                                                                   Elution of remaining molecules from a cation exchange column requires washing with pH 10 buffer.                                                                                   Dissolution of lysozyme requires pH 3 buffer to maintain activity.                                                                                   Elution of remaining molecules from an anion exchange column requires washing with pH 2 buffer.…

Match each weak acid with the pH value at which it would buffer. pH 3 ammonium (PK, of 9.25) chloroacetic acid (pK, of 2.87) acetic acid (pK₂ of 4.76) formic acid (PK, of 3.8) pH 5 Answer Bank boric acid (PK, of 9.24) pH 9 hydrazoic acid (PK, of 4.6)

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Q1: You are tasked to prepare three buffers with pH values: 9.10, 10.45, and 4.65. You have the following 0.10 M solutions available: CH3COOH, NaCH3COO, NHẠC1, NH3, NaHCO3, and Na2CO3. Which solution(s) will you choose to design each buffer? pH 4.56 pH 9.10 pH 10.45

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What is happening in this trace? What do the peaks represent? Why is absorbance measured at 230 nm?

3G 19:15 B MEDICAL AND BIOLOGICAL IMPORTANCE 1. Water is essential constituent of all forms of life. 2. Water is present in every cell. It is the medium in which all cellular events occurs. 3. It is required for enzyme action and for the transport of solutes in the body. 4. Water aids the folding of biomolecules like proteins, nucleic acids etc. Assignment One · Discuss the biological significance of water to living organisms. · Not less than 2 pages and not more than 4. (Cover page is not included in the numbering) · Submit via the google classroom. · Due date is 2nd March, 2021. · No late submissions.

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Follow up question

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Submit 3 4. 7) 9. 10 3 of 10 Question 3 Directions: Answers must be in essay form. Outline form is not acceptable. Labeled diagrams may be used to supplement discussion, but in no case will a diagram alone suffice. It is important that you read each question completely before you begin. Water is essential to all living things. (a) Discuss THREE properties of water. В I U х? X, 5 ¢ Ω 0/10000 Word Limit (b) Explain each of the following in terms of the properties of water. You are not limited to the three properties discussed in part (a): • the role of water as a medium for the metabolic processes of cells • the ability of water to moderate temperature within living organisms and in organisms'environment • the movement of water from the roots to the leaves of plants B IU x2 X2 Ω 0/10000 W II II 5

Please look at the picture. Thanks

Tables A & B. Ninhydrin and Biuret Reactions Samples TESTS Ninhydrin Biuret 1. Glycine 2. Casein 3. Albumin 4. Gelatin 5. Peptone 6. distilled water

ARAN2O H,0+ COH2 CO3E Hcot : PH- Air H,O Lab Time: 1:30 Pm 1. Knowing that these 2 molecules are always floating in the air, hypothesize why your distilled water ( which indeed was pure when put in the container and therefore should have been pH 7), instead had a pH between 5 and 6 (i.e. acidic, not neutral). 2. Hypothesize why the addition of sucrose did not change the pH of distilled water (a big hint to the answer is in the lab section on Acids & Bases).

What type of material is mycolic acid?    Question options:   ethylene glycol   nitrogenous    polysaccharide   waxy compound

Why annealing, denaturation temperatures are different?  Please answer at your own words.

Could you please assist?

PS. this is not graded.

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Enumerate the following questions about proteins1. Name at least 4 elements that made up proteins.2. Give 3 functions of protein3. Examples of denatured agents (3) of protein