Week 1 Lab Blood
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Week 1 Blood
Learning Objectives:
Identify the cellular components of blood and state their functions.
Explain the role of the white blood cells in protecting the body from disease.
Analyze complete blood counts.
Learn how to use an automatic hematology analyzer and how to prepare a peripheral
smear.
Name the 4 major blood types in the ABO system.
Explain the principles of blood typing using Eldon cards.
Describe Rh incompatibility and hemolytic disease of the newborn.
Introduction:
The body is a complex multicellular organism that comprises trillions of cells working together as
tissues, organs, and systems. These cells require a continuous supply of oxygen and nutrients
in order to function. They also need an efficient and fast means of delivery of these substances
as well as a means of removing waste byproducts.
Blood is composed of 2 components: blood plasma and the formed elements. In the first lab
simulation, you will generate complete blood counts and prepare blood smears of control and
patient samples to diagnose various blood disorders in three patients. Dive into the inner
workings of the different blood components with immersive 3D animations, where you will get
acquainted with the morphology and function of red blood cells, white blood cells, and platelets.
In the second lab you will learn the basics of antibodies. Even though antibodies are proteins
that protect us from harmful intruders, they can sometimes cause problems. You will learn about
antibody-antigen interaction and the mechanism that lies behind Rh incompatibility. You will
discover what antigens and antibodies have to do with blood type, and why mixing certain
different blood types together can cause problems. In this simulation, you will examine blood
samples from a mother and her unborn child, to determine whether or not they are compatible.
Assignment:
Part 1:
Complete
Labster
“Hematology: Introduction to Blood”
As you complete the lab, have the lab report ready to record data. The theory section of the lab
is a useful resource.
Part 2:
Complete
Labster
“Antibodies: Why are some blood types incompatible?”
As you complete the lab, have the lab report ready to record data. The theory section of the lab
is a useful resource.
Part 3:
Complete the Lab report.
Blood Lab Report
Answer the following questions:
Part 1 Lab questions:
1.
Identify the WBCs:
(1 point each)
a.
Moocyte
b.
Neutrophil
2.
Record the Data: (3 points)
Patient
CBC abnormality
Peripheral smear result
Patient 1
High platelet count.High
WBC
Intracellular parasite visible, increased
neutrophils
Patient 2
Low levels of hematocrit,
Hb, MCV, and MCH
RBC;s abnormally shated
Patient 3
Normal
Normal
3. What is the importance of a complete blood count? (1 point)
The complete blood count (CBC) is a diagnostic test that tells us how many of each type of
blood cell there are and how much of certain proteins and chemicals are in the blood. It is a
useful way to check on a patient's general health and find any problems that might be there.
4. What information does a peripheral smear add to the results of a complete blood count? (1
point)
The peripheral smear allows visualization of the different blood cells.
5. Identify the diagnosis of the 3 patients in the Part 1 Labster lab as determined by their CBC
and Peripheral Smear results. (3 points)
Patient 1: Malaria
Patient 2: Sickle Cell Anemia
Patient 3: Hemophilia
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Related Questions
Explain the significance of AB and Rh blood groups in blood transfusions
2. Discuss the structure and function of red blood cells and hemoglobin2. Classify and characterize white blood cells3.Describe the structure of platelets and explain the process of hemostasis
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Q6a. What is hematocrit measurement? What is the function of hematocrit measurement?
Q6b. What is the name of the health condition when a person has an elevated hematocrit level?
Explain in detail how elevated hematocrit levels affect blood pressure and what health consequence it may cause.
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Explain the nursing precautions to be taken prior, during and after a Blood Transfusion.?
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Asap please explain well
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This is a question for phlebotomy: Explain who to prevent and/or handle complications in blood collection.
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02: ANSWER ALL OF THE FOLLOWING QUESTIONS
1. Differentiate between RBCS & WBCS structurally and functionally?
2. What do you think are the clinical symptoms indicating that a person requires
the surgical implantation of a mechanical pacemaker?
3. Describe the structure of the heart.
4. Outline the path that blood follows through the heart and body, starting at the
superior vena cava.
5. Describe the process by which the heartbeat is regulated.
6. How are the structures of arteries, veins, and capillaries related to their func-
tion?
7. Identify the four main components of blood.
8. Explain how the structure of red blood cells, white blood cells, and platelets
relates to the function of these cells.
9. Identify the stages and structures involved in the clotting process.
10. What factors determine the compatibility of blood types for transfusion?
11. Which blood types, in terms of the A-B-O and Rh antigens, can be donated to
somebody with type AB- blood?
12. Hemophilia is a disorder in…
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CASE STUDY #2
A 2-year-old black girl is being seen by the hematologist after her pediatrician found her to be
severely anemic with splenomegaly and jaundice. Her mother gives a possible history of a “blood
problem" in her family but doesn't know for sure. Her hemoglobin electrophoresis was normal, and
the complete blood count (CBC) revealed a normocytic anemia. The platelet and white blood cell
On the peripheral smear, there are many bizarre erythrocytes, including
counts are normal.
spiculated cells. A diagnosis of pyruvate kinase deficiency is made.
5. Since in this patient pyruvate kinase is abnormal not only is less pyruvate made but intermediates above
pyruvate in the glycolytic pathway build up slowing the pathway. Which of the following products may not be
made in the appropriate amounts in the RBC because of the deficiency of pyruvate
A. Glucose
B. Oxaloacetate
C. acetyl-CoA
D. Lactate
Explanation for answer in no. 5:
?
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CASE STUDY # 2
A 2-year-old black girl is being seen by the hematologist after her pediatrician found her to be
severely anemic with splenomegaly and jaundice. Her mother gives a possible history of a “blood
problem" in her family but doesn't know for sure. Her hemoglobin electrophoresis was normal, and
the complete blood count (CBC) revealed a normocytic anemia. The platelet and white blood cell
counts are normal. On the peripheral smear, there are many bizarre erythrocytes, including
spiculated cells. A diagnosis of pyruvate kinase deficiency is made.
6. In the RBCS of the patient described above, which of the following would be expected
? A. ADP to ATP ratios would be elevated above normal.
B. NADP+ would increase relative to NADPH.
C. Ribulose 5-phosphate levels would decrease.
D. NADH to NAD+ ratios would decrease.
E. Methemoglobin levels would increase.
Explanation for answer in no. 6:
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Which information is important for the nurse to remember about essential thrombocythemia? Along with increased platelets, there may also be an increase in:
A. red blood cells.
B. Reed-Sternberg cells.
C. M protein cells.
D. mast cells.
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Q. What is a source and function of erythropoietin?
Explain: What is meant by polycythemia? What
causes polycythemia? How polycythemia would
affect the value of hematocrit?
Give example of 4 different values of Hematocrit
and explain their meaning.
Edit View Insert
Format
Tools
Table
12pt v
Paragraph v
|BIU
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CASE STUDY 1
2222
History and Physical Examination
This family had a son who had died at age 2 weeks because
of overwhelming bacterial infection. When their newborn
daughter began developing recurrent infections, she was
immediately taken to a pediatrician.
Laboratory Data
Hemoglobin and hematocrit-within normal range
Total WBC count-62.0 x 10°/L
Absolute leukocyte counts-above normal for each
leukocyte type
Leukocyte
differential-neutrophils 76%, lymphocytes
22%, cosinophils 2%
Flow cell cytometry
T lymphocytes normal proportions of CD4+ and
CD8+ cells
B lymphocytes (XA19+) elevated
Natural killer (NK) cells-elevated
CD15+ lymphocytes-absent
Serum Ig fractions within reference ranges
Treatment
The infant was given busulfan cyclophosphamide and
antithymocyte serum for 10 days. She received mature T
lymphocyte-depleted bone marrow transplanted from her
mother. This was followed by a short period of immuno-
suppressive therapy.
She recovered from the procedures and did well clinically.…
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rom
che
of
e
Name:.
Year and Section:
EXERCISE NO. 24
Demonstration of Blood Cells
Date Submitted:
Group No.:.
POST-DISCUSSION QUESTIONS
I. Answer the following prompts:
1. Record the result of the differential count, and compare with the reference
range.
2. A 45-year-old male was seen in the emergency room with complaints of
fever and chills. Blood test reveals a WBC count of 15.5 X 109/L with a
differential count of Neutrophil -78%, Lymphocyte - 20%, Monocyte-
02%. Urine analysis shows the presence of pus cells and positive protein.
Given the laboratory result, what is the probable diagnosis?
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22,25
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CASE STUDY 2
History and Physical Examination
This 6-year-old white male patient was taken to a pediatri-
cian because of recurring abscesses since the age of 1
month. The current abscesses were lanced and he was
placed on antibiotic therapy...
The patient had two brothers who had died in infancy
of infections. His parents and two sisters are healthy.
Laboratory Data
Hemoglobin and hematocrit-slightly decreased
Total leukocyte count-elevated
Differential leukocyte count-increased percentage of
segmented neutrophils
Immunoglobulin profile-polyclonal elevation of all Ig
classes
Neutrophil oxidative burst assay (DHR) activity absent
Nitroblue tetrazolium (NBT) test (automated)-reduction
of unstimulated and stimulated neutrophils
Culture of abscess revealed S. aureus
Questions
1. What does the patient's family history suggest?
a. A genetic disorder in male offspring
b. A genetic disorder in female offspring
c. Lack of leukocyte production
d. Anemia producing an immune dysfunction
What…
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lood Typing Lab Extension
DIRECTIONS: All blood type information has been collected in the pictures
ncluded for each lab station below. Remember that to determine blood type
ou are looking for areas showing agglutination (clumping). Below is an example.
B
tation #1 -Samantha
A
B
Now read the blood typing tray at each station below to determine the individual's blood type then
answer follow up questions. Please put all answers in the provided text box next to each question.
B
Rh
ation #2 - Benji
Rh
Rh
The tray to the left indicates blood type B + (B positive)
no clumping for the A protein indicates no A protein
clumping for the B protein indicates the presence of the B protein
clumping for the Rh factor indicates a positive blood type
2. What is her possible blood type genotype?
00 blood Genotype
0+
3. What blood types could she receive if a blood transfusion is needed?
1. What is Samantha's blood type phenotype?
bold has bos
4. What is Benji's blood type phenotype?
Twent toolb
OX
M
bam…
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Subpart 1-3
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what is the triage category (red, yellow, green, black) for a 26 year old patient that walks over to me, has obviously mangled arms and crying because of how painful it is, and has a respiration of 22/min, and has 124 radial pulse?
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Recall from the video the steps for determining the hematocrit. Place the steps in the correct order from left to right.
View Available Hint(s)
Hint 1. What is the last step in determining the hematocrit?
• Hint 2. What does a centrifuge do?
Reset
Help
Centrifuge the blood
for 4 to 5 minutes.
Place the tube in a
Fill the tube three-quarters
full with blood.
Seal the end with capillary
tube sealer or clay.
Place heparinized capillary
microhematocrit
tube into the blood.
centrifuge.
Determine the percent of RBCS.
Submit
Request Answer
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I need a basic understanding. Please easily clarify
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Eosinophilia
Magnification:Z6at
2.
Which chamber of the heart - left or right ventricle- has thicker walls? How does this difference
reflect the functions of these two chambers?
3.
After observing the models, identify one structural difference that you could use to differentiate
between the anterior and posterior surfaces of the heart.
4. Identify one structural difference that you could use to differentiate the right and left sides of
the heart.
44 Blood Pathologies and Heart Anatomy Laboratory 2
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1. Identify which among the evacuated tubes can be used for a complete blood count test. Explain
2. Identify which among the evacuated tubes can be used for Prothrombin Time. Explain
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Q No 5: What is difference between ABO Blood Group and Rh Blood Group System?
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ASAP S9.
How blood for different blood test are put into the right anticoagulants transported and stored practically.
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ANSWER BRIEFLY:
4. Describe the morphology of a mature platelet, life span, kinetics, normal/reference values.
5. Differentiate the 4 zones in a mature platelet by explaining the function of each.
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Hemodynamic Monitoring Case Study:
A 67-y.o woman is admitted to the ICU with a dx. of hypotension of unknown origin. She is presently unresponsive but is breathing spontaneously on her own. Lungs are clear, urinary output is 15ml in 8 hours, and her skin is cool. A PA catheter is inserted to obtain more information and guide therapy. The following information is revealed:
BP 86/54mmHg PAWP 4mmHgP 118/min CVP 2mm/HgRR 30 breaths/min SVR 1393 dynesC.O. 3.5 L/min PVR 195 dynesC.I. 1.9 L/min SvO2 50%PA 24/10mmHg
Which Values tell us about this patient's filling pressures/fluid status?
Are these values adequate?
Which Values tell us about this patient's contractility?
Are these values adequate?
Which values tell us about this patient’s vascular resistance?
What is causing this patient's hypotension?
Which initial therapy is appropriate?
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Blood group asap
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Must post first.
Bob, a 65-year-old man with atrial fibrillation, has been using warfarin for the past 12 months after he presented to the local emergency department with signs of a TIA. A head CT scan and trans-esophageal echocardiogram done at the time were normal. He has been well since.
Bob has came it the clinical today as the INR taken this morning was 4.6. Up until now, his INR results (which have been measured every 2 weeks) have been stable and in the range of 2.0√3.0. He has not started any new prescribed medications recently.
Bob also has hypertension and osteoarthritis (for which he had a left total hip replacement 6 months ago). Current medications: Atenolol 50 mg once daily, Lisinopril 10 mg once daily and warfarin 6 mg at night.
On examination: BP 140/80 mmHg; pulse rate 65, irregular. The remainder of the physical examination is normal with no evidence of bruising, epistaxis, or gastrointestinal bleeding.
List potential drug interaction(s) with warfarin, which…
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ACTIVITY NO. 6
CAPILLARY FRAGILITY TEST (Rumple Leede Test)
1. What stage/s in hemostasis is evaluated by Tourniquet Test?
2. Explain briefly the role of the following factors in maintaining vascular integrity.
2.1 Platelets
2.2 Vitamin C
3. Give 3 examples for each of the following bleeding disorders associated with vascular abnormalities
3.1 Connective Tissue Defects
3.1.1 Hereditary
3.1.2 Acquired
3.2 Altered Vessel Wall Structure
3.2.1 Hereditary
3.2.2 Acquired
4. Give a brief description of the following term related to hemostasis.
4.1 Purpura
4.2 Ecchymosis
4.3 Hematoma
5. Illustrate and label the different steps of Rumple Leede Test.
(picture for reference if answers cannot be found please refer to useful websites)
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For each of the following Patient Profiles, determine the most appropriate triage category (red, yellow, green, or black), and why.
patient profiles:
1. soaked with blood, no obvious killer bleed
- tells you he feel dizzy
- respirations : 28/min
- radial pulse : non-palpable
2. lower extremities under wooden debris
- tells you she can't move or feel her legs
- respirations : 18/min
- radial pulse : present
3. patient is face down on the floor
- unresponsive
- respirations : open airway, not breathing
- carotid pulse : weak
4. patient gurgles but can't maintain an open airway
- unresponsive
- respirations : gasps
- radial pulse : absent
5. patient has an open head wound, bleeding controlled
- unconscious
- respirations : 16/min
- CRT : 2
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I. INSTRUCTION: Analysis. Presented on this part are set of images with certain medical conditions. By looking closely and applying what you’ve learned from Module 03, try to explain/discuss/interpret the image with respect to the structure and composition of blood cells. Support your arguments using certain blood cells as biological indicators.
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- Which information is important for the nurse to remember about essential thrombocythemia? Along with increased platelets, there may also be an increase in: A. red blood cells. B. Reed-Sternberg cells. C. M protein cells. D. mast cells.arrow_forwardQ. What is a source and function of erythropoietin? Explain: What is meant by polycythemia? What causes polycythemia? How polycythemia would affect the value of hematocrit? Give example of 4 different values of Hematocrit and explain their meaning. Edit View Insert Format Tools Table 12pt v Paragraph v |BIUarrow_forwardCASE STUDY 1 2222 History and Physical Examination This family had a son who had died at age 2 weeks because of overwhelming bacterial infection. When their newborn daughter began developing recurrent infections, she was immediately taken to a pediatrician. Laboratory Data Hemoglobin and hematocrit-within normal range Total WBC count-62.0 x 10°/L Absolute leukocyte counts-above normal for each leukocyte type Leukocyte differential-neutrophils 76%, lymphocytes 22%, cosinophils 2% Flow cell cytometry T lymphocytes normal proportions of CD4+ and CD8+ cells B lymphocytes (XA19+) elevated Natural killer (NK) cells-elevated CD15+ lymphocytes-absent Serum Ig fractions within reference ranges Treatment The infant was given busulfan cyclophosphamide and antithymocyte serum for 10 days. She received mature T lymphocyte-depleted bone marrow transplanted from her mother. This was followed by a short period of immuno- suppressive therapy. She recovered from the procedures and did well clinically.…arrow_forward
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